초록 ▼

The present invention provides polyacrylamide hydrogels (especially polyacrylamide hydrogel arrays) made from prepolymers and polyacrylamide reactive polymers, as well as methods and compositions for preparing the polyacrylamide hydrogels or hydrogel arrays. The method advantageously provides greate

The present invention provides polyacrylamide hydrogels (especially polyacrylamide hydrogel arrays) made from prepolymers and polyacrylamide reactive polymers, as well as methods and compositions for preparing the polyacrylamide hydrogels or hydrogel arrays. The method advantageously provides greater control of polyacrylamide pore size and crosslink density, allowing, for instance, preparation of a polyacrylamide hydrogel appropriate for use with DNA. Furthermore, the methods and compositions can be employed in commercial processes.

대표청구항 ▼

1. A method of preparing a polyacrylamide hydrogel comprising:(a) providing a liquid solution comprising a polyacrylamide reactive prepolymer, wherein the polyacrylamide reactive prepolymer comprises a copolymer of acrylamide and one or more compounds selected from the group consisting of N-(6-acryl

1. A method of preparing a polyacrylamide hydrogel comprising:(a) providing a liquid solution comprising a polyacrylamide reactive prepolymer, wherein the polyacrylamide reactive prepolymer comprises a copolymer of acrylamide and one or more compounds selected from the group consisting of N-(6-acryloylhexyl)-2,3-dimethylmaleimide, vinyl cinnamate, and acrylic acid, wherein the acrylic acid is functionalized with a compound selected from the group consisting of glycidyl methacrylate, hydroxy ethyl acrylate, and hydroxy propyl acrylate; (b) placing a liquid solution of said polyacrylamide reactive prepolymer on a solid support; and (c) photochemically crosslinking said polyacrylamide reactive prepolymer to obtain said polyacrylamide hydrogel.