초록 ▼

The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission

The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

대표청구항 ▼

1. A method for determining the effects of at least one drug, toxin or chemical on respiring mammalian or insect cells comprising: (i) preparing a liquid media broth of said mammalian or insect cells; (ii) contacting said broth with a sensor composition which comprises a luminescent compound that e

1. A method for determining the effects of at least one drug, toxin or chemical on respiring mammalian or insect cells comprising: (i) preparing a liquid media broth of said mammalian or insect cells; (ii) contacting said broth with a sensor composition which comprises a luminescent compound that exhibits a change in luminescent property, when irradiated with light containing wavelengths which cause said compound to luminesce, upon exposure to oxygen, wherein the presence of the sensor composition is non-destructive to the mammalian or insect cells; (iii) admixing with said broth a quantity of said drug, toxin or chemical; (iv) irradiating said sensor composition with light containing wavelengths which cause said luminescent compound to luminesce; (v) measuring or visually observing the change in luminescent property from said luminescent compound while irradiating said sensor compound with said light; and (vi) comparing said measurement to that of a control wherein said control is selected from the group consisting of: a reagent control not in contact with respiring mammalian or insect cells or the drug, toxin or chemical; a reagent control in contact with respiring mammalian or insect cells but not in contact with the drug, toxin or chemical and a calculated threshold, wherein a change in luminescent property relative to the control is indicative of cytotoxicity of the drug, toxin or chemical to the mammalian or insect cells; and (vii) in the even that no such change is measured or observed, repeat steps (iv), (v) and (vi), as needed, to determine the effects of the drug, toxin or chemical on the respiring mammalian or insect cells.