초록 ▼

The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits

The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.

대표청구항 ▼

1. An apparatus for determining the presence of at least one target analyte in a liquid sample comprising:a lateral surface for receiving a liquid sample and transporting the liquid sample to a capture area on the lateral surface, wherein a portion of the lateral surface contains a mobilizable label

1. An apparatus for determining the presence of at least one target analyte in a liquid sample comprising:a lateral surface for receiving a liquid sample and transporting the liquid sample to a capture area on the lateral surface, wherein a portion of the lateral surface contains a mobilizable labeled probe comprised of an up-converting phosphor microparticle label attached to a probe which binds specifically to the target analyte to form a labeled probe-analyte complex; and wherein the capture area has immobilized thereon a capture probe capable of binding specifically to the labeled probe-analyte complex.