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Reagent useful for synthesizing sulfurized oligonucleotide analogs 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C07C-321/00
  • C07C-323/00
  • C07C-381/00
  • C07H-021/00
출원번호 US-0451913 (1999-12-01)
발명자 / 주소
  • Zacharia S. Cheruvallath
  • Vasulinga T. Ravikumar
  • Douglas L. Cole
  • Daniel C. Capaldi
출원인 / 주소
  • ISIS Pharmaceuticals, Inc.
대리인 / 주소
    Oblon, Spivak, McClelland, Maier & Neustadt, P.C.
인용정보 피인용 횟수 : 0  인용 특허 : 46

초록

The present invention is directed to a method of synthesizing sulfurized oligonucleotide analogs by reacting an oligonucleotide analog containing a phosphorous(III) linkage with a dithiocarbonic acid diester polysulfide having the formula [EQU1]to produce a sulfurized oligonucleotide analog. The die

대표청구항

1. A composition for synthesizing sulfurized oligonucleotide analogs, comprising a thiodicarbonic acid diester polysulfide represented by the formula: [EQU14]in admixture with a solid support suitable for synthesizing oligonulceotide analogs, whereineach R is independently selected from the group co

이 특허에 인용된 특허 (46)

  1. Ravikumar Vasulinga (Carlsbad CA) Andrade Mark (Carlsbad CA) Mulvey Dennis (Conroe TX) Cole Douglas L. (San Diego CA), Carbocation scavenging during oligonucleotide synthesis.
  2. Stee Wojciech J. (Lodz PLX) Grajkowski Andrzej (Lodz PLX) Uznanski Bogdan (Lodz PLX), Chalcophospholanes useful in the synthesis of oligonucleoside phosphorothioates, phosphorodithioates and related selenat.
  3. Andrus William A. (San Francisco CA) Efcavitch J. William (Belmont CA) McBride Lincoln J. (Redwood City CA), Chemical capping by phosphitylation during oligonucleotide synthesis.
  4. Groody E. Patrick (Naperville IL), Compositions and methods for the synthesis of oligonucleotides having 5′-phosphorylated termini.
  5. Cook Phillip D. (Carlsbad CA) Guinosso Charles J. (Carlsbad CA) Bruice Thomas (Carlsbad CA), Compounds useful in the synthesis of nucleic acids capable of cleaning RNA.
  6. Ecker David (Leucadia CA), Inhibiting RNA expression by forming a pseudo-half-knot RNA at the target\s RNA secondary structure using antisense olig.
  7. Ecker David J. (Leucadia CA) Wyatt Jacqueline R. (Carlsbad CA) Imbach Jean L. (Montpellier FRX), Inhibitors of human immunodeficiency virus.
  8. Bhatt Ram S. (San Diego CA), Method and reagent for sulfurization of organophosphorous compounds.
  9. Bhatt Ram S. (San Diego CA), Method and reagent for sulfurization of organophosphorous compounds.
  10. Froehler Brian C. (Belmont CA) Matteucci Mark D. (Redwood City CA), Method for in vitro oligonucleotide synthesis using H-phosphonates.
  11. Nelson Paul S. (Union City CA), Method for labeling the 3′terminus of a synthetic oligonucleotide using a unique multifunctional controlled pore glass.
  12. Beaucage Serge L. (Silver Spring MD) Regan Judith B. (Annandale VA) Iyer Radhakrishnan P. (Rockville MD), Method for the sulfurization of phosphorous groups in compounds.
  13. Stec Wojciech J. (Lodz PLX) Uznanski Bogdan (Lodz CA PLX) Bergot B. John (Redwood City CA) Hirschbein Bernard L. (San Francisco CA) Fearon Karen L. (Union City CA), Method of synethesizing sulfurized oligonucleotide analogs.
  14. Woo Sam L. (Redwood City CA) Menchen Steven M. (Fremont CA) Fung Steven (Palo Alto CA), Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports.
  15. Stec Wojciech J. (Lodz PLX) Uznanski Bogdan (Lodz CA PLX) Bergot B. John (Redwood City CA) Hirschbein Bernard L. (San Francisco CA) Fearson Karen L. (Union City CA), Method of synthesizing sulfurized oligonucleotide analogs.
  16. Hirschbein Bernard L. (San Francisco CA), Method of synthesizing sulfurized oligonucleotide analogs with thiuram disulfides.
  17. Urdea Michael S. (Alamo CA) Horn Thomas (Berkeley CA), Method of using colored phosphorylating reagents.
  18. Caruthers Marvin H. (Boulder CO) Ma Yun-Xi (Mississauga WA CAX) Yau Eric K. (Kirkland WA) Nielsen John (Horsholm DKX) Brill Wolfgang (Freiburg DEX), Nucleoside thiophosphoramidites.
  19. Caruthers Marvin H. (Boulder CO) Matteucci Mark D. (San Francisco CA), Nucleosides useful in the preparation of polynucleotides.
  20. Crea Roberto (Burlingame CA), Nucleosidic phosphorylating agent and methods.
  21. Bennett C. Frank (Carlsbad CA) Mirabelli Christopher K. (Encinitas CA), Oligonucleotide modulation of cell adhesion.
  22. Baracchini ; Jr. Edgardo (San Diego CA) Bennett Clarence F. (Carlsbad CA), Oligonucleotide modulation of multidrug resistance-associated protein.
  23. Caruthers Marvin H. (Boulder CO) Beaucage Serge L. (Boulder CO), Phosphoramidite compounds and processes.
  24. Caruthers Marvin H. (Boulder CO) Beaucage Serge L. (Mountain View CA), Phosphoramidite nucleoside compounds.
  25. Chasar Dwight W. (Northfield OH), Polyphosphoramidite oligomers and stabilizer compositions thereof.
  26. Hsiung Hansen M. (Indianapolis IN), Process for de-cyanoethylating blocked nucleotides.
  27. Caruthers Marvin H. (Boulder CO) Beaucage Serge L. (Mountain View CA), Process for oligonucleo tide synthesis using phosphormidite intermediates.
  28. Gorenstein David (Carmel IN) Farschtschi Nassar (West Lafayette IN), Process for preparing dithiophosphate oligonucleotide analogs via nucleoside thiophosphoramidite intermediates.
  29. Ravikumar Vasulinga T. (Carlsbad CA) Mulvey Dennis (Vista CA) Cole Douglas L. (San Diego CA) Cook Phillip D. (Carlsbad CA), Process for preparing oligonucleotides using silyl-containing diamino phosphorous reagents.
  30. Caruthers Marvin H. (Boulder CO) Matteucci Mark D. (Boulder CO), Process for preparing polynucleotides.
  31. Caruthers Marvin H. (Boulder CO) Matteucci Mark D. (Boulder CO), Process for preparing polynucleotides.
  32. Kster Hubert (Concord MA) Sinha Nanda D. (San Rafael CA), Process for the preparation of oligonucleotides.
  33. Kster Hubert (Hamburg DEX) Sinha Nanda D. (Bundu Dt. Ranchi INX), Process for the preparation of oligonucleotides.
  34. Frank Ronald (Wolfenbttel DEX) Heikens Wiebke (Braunschweig DEX), Process for the simultaneous synthesis of several oligonucleotides on a solid phase.
  35. Cheruvallath Zacharia S. ; Capaldi Daniel C. ; Ravikumar Vasulinga T. ; Cole Douglas L., Protecting group for synthesizing oligonucleotide analogs.
  36. Cheruvallath Zacharia S. ; Capaldi Daniel C. ; Ravikumar Vasulinga T. ; Cole Douglas L., Protecting group for synthesizing oligonucleotide analogs.
  37. Cheruvallath Zacharia S. ; Capaldi Daniel C. ; Ravikumar Vasulinga T. ; Cole Douglas L., Protecting group for synthesizing oligonucleotide analogs.
  38. Cheruvallath Zacharia S. ; Ravikumar Vasulinga T. ; Cole Douglas L. ; Capaldi Daniel C., Reagent useful for synthesizing sulfurized oligonucleotide analogs.
  39. Cheruvallath Zacharia S. ; Ravikumar Vasulinga T. ; Cole Douglas L. ; Capaldi Daniel C., Reagent useful for synthesizing sulfurized oligonucleotide analogs.
  40. Kempe Tomas (Bowie MD), Recovery of oligonucleotides by gas phase cleavage.
  41. Stec Wojciech J. (Lodz PLX) Grajkowski Andrzej (Lodz PLX) Uznanski Bogdan (Lodz PLX), Solid phase oligonucleotide synthesis using phospholane intermediates.
  42. Zeiger Allen R. (Philadelphia PA), Solid phase polynucleotide syntheses.
  43. Itakura Keiichi (Arcadia CA), Solid-phase synthesis of polynucleotides.
  44. Itakura Keiichi (Arcadia CA), Solid-phase synthesis of polynucleotides.
  45. Southern Edwin M. (Oxford GB3) Maskos Uwe (Oxford GB3), Support-bound oligonucleotides.
  46. Ravikumar Vasulinga (Carlsbad CA) Cole Douglas L. (San Diego CA), Synthesis of oligonucleotides.
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