초록 ▼

The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods com

The present invention provides a method of compiling a plant functional gene profile, a method of changing the phenotype or biochemistry of a plant, a method of determining a change in phenotype or biochemistry of a plant, and a method of determining the presence of a trait in plant. The methods comprise expressing transiently a nucleic acid sequence of a plant into a host plant to affect phenotypic or biochemical changes in the host plant. A viral vector functional genomic screen has been developed to identify nucleotide sequences in transfected plants by systemically knocking out endogenous gene expression in an antisense mechanism. Once the presence of a trait in a plant is identified by phenotypic or biochemical changes in the host plant, the nucleic acid insert in the cDNA clone or in the vector that results in the changes is then sequenced. The present invention exemplifies that genes encoding GTP binding proteins in one plant can silence endogenous gene expression in a different plant.

대표청구항 ▼

1. A method of compiling a plant antisense functional gene profile comprising:a) preparing a library of DNA or RNA sequences from a donor plant, and constucting recombinant viral nucleic acids obtained from a tobamo virus comprising an unidentified nucleic acid insert obtained from said library in a

1. A method of compiling a plant antisense functional gene profile comprising:a) preparing a library of DNA or RNA sequences from a donor plant, and constucting recombinant viral nucleic acids obtained from a tobamo virus comprising an unidentified nucleic acid insert obtained from said library in an antsense orientation relative to said DNA or RNA sequence of said donor plant; b) infecting a plant host with one or more said recombinant viral nucleic acids; c) transiently expressing said unidentified nucleic acid in the plant host; d) determining one or more phenotypic or biochemical changes in the plant host, if any; e) identifying an associated trait where a phenotypic or biochemical change occurs; f) identifying said recombinant viral nucleic acid that results in said one or more changes in the plant host; g) identifying a plant host gene, if any, associated with the trait, and h) repeating steps b)-g) until at least one nucleic acid is associated with said trait, whereby an antisense functional gene profile of the plant host or of the donor plant is compiled.