초록 ▼

The present invention provides an apparatus and method for disrupting cells or viruses to release the nucleic acid therefrom. The apparatus includes a container having a chamber for holding the cells or viruses. The apparatus also includes an ultrasonic transducer for contacting a wall of the chambe

The present invention provides an apparatus and method for disrupting cells or viruses to release the nucleic acid therefrom. The apparatus includes a container having a chamber for holding the cells or viruses. The apparatus also includes an ultrasonic transducer for contacting a wall of the chamber and for transmitting ultrasonic energy into the chamber through the wall. A support structure holds the container and the transducer against each other such that the transducer contacts the wall of the chamber. The support structure includes an elastic body, such as a spring, for applying to the container or to the transducer a substantially constant force to press together the transducer and the wall. The chamber also preferably contains beads for enhancing the disruption of the cells or viruses. The apparatus performs rapid and consistent lysis of cells or viruses, often in as little time as 5 to 10 seconds.

대표청구항 ▼

1. An apparatus for disrupting cells or viruses, the apparatus comprising:a) a container having a chamber for holding the cells or viruses, wherein the container includes at least one wall defining the chamber; b) an ultrasonic transducer for contacting an external surface of the wall and for transm

1. An apparatus for disrupting cells or viruses, the apparatus comprising:a) a container having a chamber for holding the cells or viruses, wherein the container includes at least one wall defining the chamber; b) an ultrasonic transducer for contacting an external surface of the wall and for transmitting ultrasonic energy into the chamber through the wall; and c) a support structure for holding the container and the transducer against each other such that the transducer contacts the external surface of the wall and for applying to the container or to the transducer a substantially constant force to press together the transducer and the wall.