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A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an .alpha.-helical coiled-coil het

A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an .alpha.-helical coiled-coil heterodimer. The-first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.

대표청구항 ▼

1. A array device comprisingan assay support surface defining an array of surface regions, a two-subunit heterodimer complex composed of first and second peptides that together form an .alpha.-helical coiled-coil heterodimer carried on each of said regions where said first peptide is attached to eac

1. A array device comprisingan assay support surface defining an array of surface regions, a two-subunit heterodimer complex composed of first and second peptides that together form an .alpha.-helical coiled-coil heterodimer carried on each of said regions where said first peptide is attached to each surface region, and a region-specific ligand covalently attached to the second peptide in the two-subunit heterodimer complex in each region, and accessible for binding by a ligand-binding agent.