초록 ▼

A process and kit which permit quantitative measurement in a single analysis step of a target antigen of a whole cell. The process and kit utilize a solid support onto which is bound at least one antibody that binds to and immobilizes the subject whole cell at a surface antigenic site other than the

A process and kit which permit quantitative measurement in a single analysis step of a target antigen of a whole cell. The process and kit utilize a solid support onto which is bound at least one antibody that binds to and immobilizes the subject whole cell at a surface antigenic site other than the target antigen. A second, labeled antibody specific for the target antigen is then added, and the binding is then quantitatively measured by analytical means. The process of this invention can provide rapid quantitative assay, e.g., less than one hour, of multiple samples.

대표청구항 ▼

1. A kit for quantitative determination of specific antigens of T and B lymphocytic subpopulations, which consists essentially of:a) a plurality of solid supports to which are bound one or more monoclonal capture antibodies that specifically bind to surface antigens, other than the antigen to be det

1. A kit for quantitative determination of specific antigens of T and B lymphocytic subpopulations, which consists essentially of:a) a plurality of solid supports to which are bound one or more monoclonal capture antibodies that specifically bind to surface antigens, other than the antigen to be determined, characteristic of a T or B lymphocytic subpopulation; b) a solution of a monoclonal probe antibody that specifically binds to a surface antigen to be determined of the T or B lymphocytic subpopulation, the monoclonal probe antibody being labeled with a radioisotope label or an enzyme label; and c) a series of reference samples containing appropriate cells bearing both the antigens recognized by said capture antibodies and known amounts of the surface antigen to be determined, wherein each solid support is a well in a multiwell plate in which one or more monoclonal capture antibodies specific for surface antigens of said T or B lymphocytic subpopulation have been fixed, and wherein the kit comprises a plurality of monoclonal probe antibody containing solutions each containing a different monoclonal probe antibody specific for a surface antigen of said T or B lymphocytic subpopulation.