초록 ▼

Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in at

Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.

대표청구항 ▼

1. An activated slide comprising a support surface coated with a bound composition of a reagent composition, the bound composition comprising a polymeric backbone having more than one pendent thermochemically amine-reactive or sulfhydryl-reactive groups configured and arranged to form covalent bonds

1. An activated slide comprising a support surface coated with a bound composition of a reagent composition, the bound composition comprising a polymeric backbone having more than one pendent thermochemically amine-reactive or sulfhydryl-reactive groups configured and arranged to form covalent bonds with fundamental groups on a target molecule, the reagent composition being coated and immobilized on the surface in a manner that permits: a) small sample volume of a solution containing the target molecule to be applied in the form of a discrete spot on the reagent composition-coated surface, b) target molecule present in the sample volume to become attached to the bound composition by reaction between the functional groups of the target molecule and the thermochemically amine-reactive or sulfhydryl-reactive groups of the bound composition, and c) substantially all unattached target molecule to be washed from the sport without undue detectable amounts of target molecule in the area surrounding the spot, and wherein the bound composition is configured and arranged to form covalent bonds with functional groups on the target molecule without the use of attracting groups to attract the target molecule to the bound composition.