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Methods of preserving prokaryotic cells and compositions obtained thereby 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12N-001/04
출원번호 US-0985343 (1997-12-04)
발명자 / 주소
  • Alan G. Tunnacliffe GB
  • David T. Welsh GB
  • Bruce J. Roser GB
  • Kamaljit S. Dhaliwal GB
  • Camilo Colaco GB
출원인 / 주소
  • Quadrant Healthcare (UK) Limited GB
대리인 / 주소
    Morrison & Forester LLP
인용정보 피인용 횟수 : 37  인용 특허 : 24

초록 ▼

This invention provides methods of drying and stabilizing prokaryotic cells, and the compositions obtained thereby. The cells are first cultured or incubated under conditions sufficient to induce intracellular trehalose, suspended in a stabilizing solution and dried to form a solid glass. The result

대표청구항 ▼

1. A method of preserving prokaryotic cells comprising the steps of:a) increasing intracellular trehalose concentration in the prokaryotic cells to at least 30 mM; b) mixing the prokaryotic cells obtained in step a) with a drying solution comprising a stabilizing agent, wherein the stabilizing agent

이 특허에 인용된 특허 (24)

  1. Nishikawa Yoshihiro (Kanazawa JPX) Kanemitsu Kimihiro (Kanazawa JPX) Katori Tatsuhiko (Ibaragi JPX) Shibata Akihiro (Narashino JPX) Kukita Kenichi (Kashiwa JPX), aa 상세보기
  • Livesey Stephen A.,AUX ; del Campo Anthony A. ; Nag Abhijit ; Nichols Ken B. ; Piunno Carmen ; Ross David P., Apparatus for cryopreparation, dry stabilization and rehydration of biological suspensions.
    상세보기
  • Shimada Shizuo (Mobara JPX) Sudo Tadashi (Mobara JPX) Inoue Hitoshi (Mobara JPX) Furutani Yoshio (Yokohama JPX) Fujisawa Yoshikazu (Kamakura JPX), Bacterial cell extract, process for preparing same, antitumor preparation containing same, and adjuvant preparation cont.
    상세보기
  • Drake Cyril F. (Harlow GB2) Arch Alfred J. (Ongar GB2), Controlled delivery device.
    상세보기
  • Myers Garry L. (Reston VA) Battist Gerald E. (Reston VA) Fuisz Richard C. (Great Falls VA), Delivery of controlled-release systems(s).
    상세보기
  • Shibata Akihiro (Narashino JPX) Matsuda Hideaki (Abiko JPX) Kohya Hidehiko (Sendai JPX) Katori Tatsuhiko (Tone JPX), Derivative of aa 상세보기
  • Shishov Nikolai M. (Moscow SUX) Zelenetsky Vladimir E. (Moscow SUX) Demina Nadezhda A. (Moscow SUX) Bondarev Ivan M. (Moscow SUX) Cherny Alexandr N. (Moscow SUX) Moskvitin Valery A. (Belgorod-Dnestro, Device for administering powdered substances.
    상세보기
  • Roser Bruce J. (Cambridgeshire GB3), Dried food containing trehalose and method for preparing same.
    상세보기
  • Cleland Jeffrey L. (San Carlos CA) Jones Andrew J. S. (San Mateo CA), Excipient stabilization of polypeptides treated with organic solvents.
    상세보기
  • Goodrich ; Jr. Raymond P. (Pasadena CA) Williams Christine M. (Pasadena CA), Method of reconstituting lyophilized cells.
    상세보기
  • Zema Marco (Como ITX) Mapelli Luigi G. (Milan ITX) Marconi Marco G. (Milan ITX), Pharmaceutical composition for rapid suspension in water.
    상세보기
  • Johnson Eric A. (Madison WI) Goodnough Michael C. (Madison WI), Pharmaceutical composition of botulinum neurotoxin and method of preparation.
    상세보기
  • Bronshtein Victor, Preservation by foam formation.
    상세보기
  • Roser Bruce J. (Balsham GB3), Preservation of viruses.
    상세보기
  • Bogue B. Arlie (Broad Run VA) Fuisz Richard C. (Great Falls VA) Hiscocks Peter G. (Saffron Walden GB2), Process for making shearform matrix.
    상세보기
  • Roser Bruce J. (Balsham GB3), Protection of proteins and the like.
    상세보기
  • Fuisz Richard C. (Bethlehem PA), Rapidly dissoluble medicinal dosage unit and method of manufacture.
    상세보기
  • Burke Rae L. (San Francisco CA) Pachl Carol (Oakland CA) Valenzuela Pablo D. T. (San Francisco CA), Recombinant herpes simplex gb-gd vaccine.
    상세보기
  • Ribi Edgar E. (Hamilton MT) Schwartzman Steven M. (Stevensville MT) Cantrell John L. (Hamilton MT), Refined detoxified endotoxin product.
    상세보기
  • Fuisz Richard C. (Great Falls VA), Saccharide-based matrix.
    상세보기
  • Kensil Charlotte A. (Milford MA) Marciani Dante J. (Hopkinton MA), Saponin adjuvant.
    상세보기
  • Loria Roger M. (Richmond VA) Regelson William (Richmond VA), Use of DHEA as a medicinal.
    상세보기
  • Loria Roger M. (P.O. Box 678 Richmond VA 23298) Regelson William (P.O. Box 678 Richmond VA 23298), Use of dehydroepiandrosterone to improve immune response.
    상세보기
  • Legg Michael Jeremy,GB2, Viability of bacterial dried cells.
    상세보기

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    3. Harel, Mordechi; Kohavi-Beck, Keren, Delivery vehicle for probiotic bacteria comprising a dry matrix of polysaccharides, saccharides and polyols in a glass form and methods of making same.
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    4. Harel, Mordechi; Kohavi-Beck, Keren, Delivery vehicle for probiotic bacteria comprising a dry matrix of polysaccharides, saccharides and polyols in a glass form and methods of making same.
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    5. Harel, Moti; Bennett, Alicia, Dry food product containing live probiotic.
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    6. Harel, Moti; Bennett, Alicia, Dry food product containing live probiotic.
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    7. Harel, Moti; Drewes, Roger; Scarbrough, January, Dry glassy composition comprising a bioactive material.
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    8. Harel, Moti; Tang, Qiong, Dry storage stabilizing composition for biological materials.
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    9. Tarara, Thomas E.; Weers, Jeffry G.; Kabalnov, Alexey; Schutt, Ernest G.; Dellamary, Luis A., Engineered particles and methods of use.
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    10. Tarara,Thomas E.; Weers,Jeffry G.; Kabalnov,Alexey; Schutt,Ernest G.; Dellamary,Luis A., Engineered particles and methods of use.
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    11. Tarara, Thomas E.; Weers, Jeffry G.; Eldon, Michael A.; Narashimhan, Rangachari; Clark, Andrew, Formulation for pulmonary administration of antifungal agents, and associated methods of manufacture and use.
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    12. Hyde, Roderick A.; Wood, Jr., Lowell L., Glassy compositions.
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    13. Oshita,Katsumi; Fukui,Nobuyuki; Yomo,Hideko; Umezawa,Yusuke, Method of producing active dry yeast.
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    14. Patton, John S.; Foster, Linda S.; Platz, Robert M., Methods and compositions for pulmonary delivery of insulin.
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    15. Harel, Moti; Carpenter, Brian, Microparticulated vaccines for the oral or nasal vaccination and boostering of animals including fish.
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    16. de Costa, Samodh; Roser, Bruce; Sen, Shevanti, Pharmaceutical device for the administration of substances to patients.
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    17. Weers, Jeffry G.; Tarara, Thomas E.; Dellamary, Luis A.; Riess, Jean G.; Schutt, Ernest G., Phospholipid-based powders for drug delivery.
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    18. Weers, Jeffry G; Tarara, Thomas E; Dellamary, Luis A; Riess, Jean G; Schutt, Ernest G, Phospholipid-based powders for drug delivery.
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    19. DePablo, Juan; Miller, Danforth; Conrad, Paul; Corti, Horacio, Preservation and storage medium for biological materials.
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    20. DePablo, Juan; Miller, Danforth; Conrad, Paul; Corti, Horacio, Preservation and storage medium for biological materials.
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    21. Vehring, Reinhard; Ao, Yi, Preservation of bioactive materials by freeze dried foam.
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    22. Patton,John S., Pulmonary delivery of active fragments of parathyroid hormone.
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    23. Tarara, Thomas E.; Weers, Jeffry G.; Venthoye, Geraldine, Pulmonary delivery of aminoglycoside.
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    24. Tarara, Thomas E; Weers, Jeffry G; Venthoye, Maria Geraldine, Pulmonary delivery of aminoglycoside.
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    25. Tarara, Thomas E.; Weers, Jeffrey G.; Kabalnov, Alexey; Schutt, Ernest G.; Dellamary, Luis A., Pulmonary delivery particles comprising an active agent.
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    26. Baker, Tony, Removal of molecular assay interferences.
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    27. Weers, Jeffry G.; Schutt, Ernest G.; Dellamary, Luis A.; Tarara, Thomas E.; Kabalnov, Alexey, Respiratory dispersion for metered dose inhalers comprising perforated microstructures.
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    28. Roser, Bruce J.; Kampinga, Jaap; Colaco, Camilo; Blair, Julian, Solid dose delivery vehicle and methods of making same.
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    29. Roser, Bruce J.; Kampinga, Jaap; Colaco, Camilo; Blair, Julian, Solid dose delivery vehicle and methods of making same.
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    30. Roser, Bruce J.; Kampinga, Jaap; Colaco, Camilo; Blair, Julian, Solid dose delivery vehicle and methods of making same.
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    31. Weers, Jeffry G.; Schutt, Ernest G.; Dellamary, Luis; Tarara, Thomas E.; Kabalnov, Alexey, Stabilized preparations for use in metered dose inhalers.
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    32. Harel, Moti; Tang, Qiong; Rice, Trisha; Jennings, Kimberly; Carpenter, Brian; Drewes, Roger; Raditsis, Elizabeth; Scarbrough, January, Stabilizing composition for biological materials.
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    33. Harel, Moti; Tang, Qiong; Rice, Trisha; Jennings, Kimberly; Carpenter, Brian; Drewes, Roger; Raditsis, Elizabeth; Scarbrough, January, Stabilizing composition for biological materials.
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    34. Dellamary, Luis A.; Reiss, Jean; Schutt, Ernest G.; Weers, Jeffry G.; Tarara, Thomas E., Stable metal ion-lipid powdered pharmaceutical compositions for drug delivery.
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    35. Dellamary, Luis A.; Riess, Jean; Schutt, Ernest G.; Weers, Jeffry G.; Tarara, Thomas E., Stable metal ion-lipid powdered pharmaceutical compositions for drug delivery and methods of use.
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    36. Dellamary, Luis A; Riess, Jean; Schutt, Ernest G; Weers, Jeffry G; Tarara, Thomas E, Stable metal ion-lipid powdered pharmaceutical compositions for drug delivery and methods of use.
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    37. Zhang, Rui, Thermal cycling device with phase changing fluids.
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