초록 ▼

The present invention provides rapid and highly effective methods for extracting nucleic acids suitable for PCR amplification from tissue samples and paraffin-embedded tissue samples. Extraction is accomplished within a few minutes using a composition comprising a buffer, at least one nonionic surfa

The present invention provides rapid and highly effective methods for extracting nucleic acids suitable for PCR amplification from tissue samples and paraffin-embedded tissue samples. Extraction is accomplished within a few minutes using a composition comprising a buffer, at least one nonionic surfactant, and a protease enzyme. The sample is then heated at alkaline pH and after a centrifugation step, the DNA is the supernatant can be used directly in known amplification methods, such as PCR.

대표청구항 ▼

1. A method of extracting nucleic acids from tissue samples comprising contacting said sample with a buffer, at least one nonionic surfactant, and a protease enzyme under conditions sufficient to release said nucleic acids from said sample;adding alkali and heating said sample at an alkaline pH for

1. A method of extracting nucleic acids from tissue samples comprising contacting said sample with a buffer, at least one nonionic surfactant, and a protease enzyme under conditions sufficient to release said nucleic acids from said sample;adding alkali and heating said sample at an alkaline pH for a period of time sufficient to inactivate said protease enzyme; and centrifuging said sample to isolate said nucleic acids in supernatant.