초록 ▼

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

대표청구항 ▼

1. A method for extracting autologous thrombin from a patient, the steps consisting of:obtaining a blood product from the patient; sequestering plasma from the blood product; adding ethanol to the plasma to prepare a solution containing prothrombin, wherein ethanol is present in the solution at a co

1. A method for extracting autologous thrombin from a patient, the steps consisting of:obtaining a blood product from the patient; sequestering plasma from the blood product; adding ethanol to the plasma to prepare a solution containing prothrombin, wherein ethanol is present in the solution at a concentration between about 8% and about 20% volume per unit volume; converting the prothrombin in the solution to thrombin; filtering the thrombin to remove particulate matter; and applying the thrombin to the patient.