초록 ▼

The invention provides a method of improving the binding of a series of consecutive nucleotide bases to a complementary target nucleic acid molecule in a sample, wherein said method comprises at least the step or steps of binding a complementary modular oligonucleotide of at least two parts (modules

The invention provides a method of improving the binding of a series of consecutive nucleotide bases to a complementary target nucleic acid molecule in a sample, wherein said method comprises at least the step or steps of binding a complementary modular oligonucleotide of at least two parts (modules) including said nucleotide bases to adjacent stretches of said target nucleic acid molecule in said sample, especially methods of detection/isolation, particularly in the isolation of primer extension products and methods in which the modular oligonucleotide is a primer, modular oligonucleotides themselves and their use in methods of the invention.

대표청구항 ▼

1. A method for isolating primer extension products, produced from a template vector, said products containing sequences corresponding or complementary to i) a primer binding region, ii) an insert and iii) vector-derived sequence(s), wherein said method comprises:a) binding a modular oligonucleotide

1. A method for isolating primer extension products, produced from a template vector, said products containing sequences corresponding or complementary to i) a primer binding region, ii) an insert and iii) vector-derived sequence(s), wherein said method comprises:a) binding a modular oligonucleotide comprising at least two modules to adjacent stretches on said primer extension products, wherein said modular oligonucleotide is complementary to and capable of binding to said vector-derived sequence of said primer extension products, wherein at least one of said modules is a capture module which is immobilized or has means for immobilization; and b) isolating said primer extension products bound to said modular oligonucleotide.