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A multi-well filtration or solid phase extraction apparatus is provided which generally includes a vacuum collar adapted to be interconnected between a conventional 96-well sample application plate, or separation plate, and a 96-well collection plate. The vacuum collar provides an interface chamber

A multi-well filtration or solid phase extraction apparatus is provided which generally includes a vacuum collar adapted to be interconnected between a conventional 96-well sample application plate, or separation plate, and a 96-well collection plate. The vacuum collar provides an interface chamber between these plates through which a vacuum can be applied to assist in the filtration process. The collection plate may include a vent opening providing a direct communication between the interface chamber and a vacuum assembly air intake, when the collection plate is disposed on top of the vacuum assembly in a stacked configuration, thereby eliminating any need for separate vacuum manifolds or connectors.

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1. A multi-well filtration/solid phase extraction apparatus for use with a multi-well separation plate and a multi-well collection plate, the apparatus comprising:a vacuum collar adapted to be stacked between a multi-well separation plate and to a multi-well collection plate, the vacuum collar inclu

1. A multi-well filtration/solid phase extraction apparatus for use with a multi-well separation plate and a multi-well collection plate, the apparatus comprising:a vacuum collar adapted to be stacked between a multi-well separation plate and to a multi-well collection plate, the vacuum collar including a chamber providing a substantially air tight interface between the multi-well separation plate and the multi-well collection plate when the vacuum collar is stacked between the multi-well separation plate and the multi-well collection plate; and a connector for connecting the vacuum collar chamber to a vacuum source in order to deliver vacuum within the collection plate.