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A controlled atmosphere incubator having an interior chamber surrounded by a heated water jacket. A glass access door of the chamber is directly heated by a clear, electrically conductive coating. The door is sealed against the perimeter of the opening by a readily replaceable gasket and is field re

A controlled atmosphere incubator having an interior chamber surrounded by a heated water jacket. A glass access door of the chamber is directly heated by a clear, electrically conductive coating. The door is sealed against the perimeter of the opening by a readily replaceable gasket and is field reversible due to unique hinge mounting assemblies. An easily accessed blower assembly is located within the chamber and includes a HEPA filter readily replaceable by the user from within the chamber. A filtered air exchange system is provided for limiting the maximum level of humidity in the chamber. The incubator control maintains constant power output from the blower motor so that the heat output of the motor is also constant. A voltage compensated temperature control is also provided for the heaters associated with the water jacket. Compensation for environmental conditions inside the chamber is also provided by the control for producing more accurate readings of carbon dioxide levels. When an infrared carbon dioxide sensor is used, the control provides a unique calibration method which does not necessitate the use of conventional tanks of calibration gas mixtures.

대표청구항 ▼

A controlled atmosphere incubator having an interior chamber surrounded by a heated water jacket. A glass access door of the chamber is directly heated by a clear, electrically conductive coating. The door is sealed against the perimeter of the opening by a readily replaceable gasket and is field re

A controlled atmosphere incubator having an interior chamber surrounded by a heated water jacket. A glass access door of the chamber is directly heated by a clear, electrically conductive coating. The door is sealed against the perimeter of the opening by a readily replaceable gasket and is field reversible due to unique hinge mounting assemblies. An easily accessed blower assembly is located within the chamber and includes a HEPA filter readily replaceable by the user from within the chamber. A filtered air exchange system is provided for limiting the maximum level of humidity in the chamber. The incubator control maintains constant power output from the blower motor so that the heat output of the motor is also constant. A voltage compensated temperature control is also provided for the heaters associated with the water jacket. Compensation for environmental conditions inside the chamber is also provided by the control for producing more accurate readings of carbon dioxide levels. When an infrared carbon dioxide sensor is used, the control provides a unique calibration method which does not necessitate the use of conventional tanks of calibration gas mixtures. ast one of the plus and minus amplified strands by extension of a sequencing primer which hybridizes with the plus or minus amplified strand in the presence of a chain-terminating nucleotide, wherein the first amplification primer has the sequence given by Seq. ID. No. 2, and the sequencing primer has the sequence given by Seq. ID No. 3, and wherein an additional amplification primer and an additional sequencing primer specific to HPV51 are used in steps (a) and (b), respectively, and wherein the additional sequencing primer is SEQ ID No. 5. 3. A kit for determining the sequence of human papillomavirus comprising, in packaged combination: (a) first and second amplification primers for amplifying a portion of the L1 open reading frame of human papillomavirus genome to form an L1 amplicon containing plus and minus amplified strands, wherein the second amplification primer has the sequence given by Seq. ID No. 1; (b) a sequencing primer which hybridizes with the plus or minus amplified strand in the presence of a chain-terminating nucleotide for determining the positions of at least one species of nucleotide sequence within at least one of the plus and minus amplified strands by extension of the sequencing primer, wherein the first amplification primer has the sequence given by Seq. ID. No. 2, and the sequencing primer has the sequence given by Seq. ID No. 3; (c) a third amplification primer having the sequence given by Seq ID No. 4; and (d) an additional sequencing primer having the sequence given by Seq ID No. 5.