초록 ▼

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

대표청구항 ▼

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one

The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. ), pALK941 (DSM9902), pALK1056 (DSM9903) or a xylanolytic fragment thereof and wherein said host cell has been transformed with a recombinant vector that encodes said xylanase. 6. The method of claim 5, wherein said host is Trichoderma. 7. The method of claim 6, wherein said Trichoderma is Trichoderma reesei. 8. The method of claim 5, wherein the temperature is 70° C. 9. A method for chemically treating plant biomass comprising contacting said biomass with culture medium, wherein said culture medium is obtained from culture of a recombinant host cell which is not Actinomadura flexuosa, said culture medium comprising a thermostable xylanase which is active at 50-60° C., wherein the amino acid sequence of said xylanase comprises an amino acid sequence of SEQ ID NO. 2 (FIGS. 13-13A) or a xylanolytic fragment thereof and wherein said host cell has been transformed with a recombinant vector that encodes said xylanase. 10. The method of claim 9, wherein said host is Trichoderma. 11. The method of claim 10, wherein said Trichoderma is Trichoderma reesei. 12. The method of claim 9, wherein the temperature is 70° C. 13. A method for chemically treating plant biomass comprising contacting said biomass with culture medium, wherein said culture medium is obtained from culture of a recombinant host cell which is not Actinomadura flexuosa, said culture medium comprising a thermostable xylanase which is active at 50-80° C., wherein the amino acid sequence of said xylanase comprises an amino acid sequence encoded by the nucleic acid sequence of a plasmid selected from the group consisting of pALAK923 (DSM9322), pALK938 (DSM9899), pALK939 (DSM9900), pALK940 (DSM9901), pALK941 (DSM9902), pALK1056 (DSM9903) or a xylanolytic fragment thereof and wherein said host cell has been transformed with a recombinant vector that encodes said xylanase. 14. The method of claim 13, wherein said host is Trichoderma. 15. The method of claim 14, wherein said Trichoderma is Trichoderma reesei. 16. The method of claim 13, wherein the temperature is 70° C.