초록 ▼

Polyphosphates control the growth of bacteria during food processing. These compositions comprise antimicrobial polyphosphates alone or in combination with organic acids and/or their salts and are effective in food-related applications, including: cleaning compositions to disinfect the surface of fo

Polyphosphates control the growth of bacteria during food processing. These compositions comprise antimicrobial polyphosphates alone or in combination with organic acids and/or their salts and are effective in food-related applications, including: cleaning compositions to disinfect the surface of food and to clean and disinfect equipment used in food processing, and compositions to be added to food to inhibit the growth of microorganisms responsible for foodborne diseases and spoilage during distribution and storage.

대표청구항 ▼

Polyphosphates control the growth of bacteria during food processing. These compositions comprise antimicrobial polyphosphates alone or in combination with organic acids and/or their salts and are effective in food-related applications, including: cleaning compositions to disinfect the surface of fo

Polyphosphates control the growth of bacteria during food processing. These compositions comprise antimicrobial polyphosphates alone or in combination with organic acids and/or their salts and are effective in food-related applications, including: cleaning compositions to disinfect the surface of food and to clean and disinfect equipment used in food processing, and compositions to be added to food to inhibit the growth of microorganisms responsible for foodborne diseases and spoilage during distribution and storage. 31 protease not having this substitution; and (b) one or more cosmetic materials compatible with said mutant protease. 4. The cosmetic composition of claim 3, wherein the mutant protease has an amino acid substitution at position 99 relative to the unmodified Bacillus lentus alkaline protease and the substituent amino acid is selected from glycine, alagite, or serine. 5. The cosmetic composition of claim 3, wherein the mutant protease has an amino acid substitution at position 154 relative to the unmodified Bacillus lentus alkaline protease and the substituent amino acid is glutanic acid or aspartic acid. 6. The cosmetic composition of claim 3, wherein the mutant protease as an amino acid substitution at position 211 relative to the unmodified Bacillus lentus alkaline protease and the substituent amino acid is glutamic acid or aspartic acid. 7. The cosmetic composition of claim 3, wherein the mutant protease has one or more mutations selected from the group consisting of R99G, R99S, R99A, L211D), L211E, S154D, and S154E. 8. A method of avoiding or reducing tissue surface irritation due to contact with a protease containing cosmetic composition, comprising contacting a tissue surface with a cosmetic composition comprising at least one mutant Bacillus lentus alkaline protease, wherein the mutant protease comprises a Bacillus lentus alkaline protease having one or more no acid substitutions in its amino acid sequence, wherein at least one of the substitutions (i) occurs at a position lying within a 7 .ANG. radius of the substrate binding region of the unmodified Bacillus lentus alkaline protease, (ii) leads to a reduced positive charge or an increased negative charge and (iii) results in the mutant protease having reduced tissue surface irritation relative to the modified or unmodified Bacillus lentus alkaline protease not having these substitutions within the 7 .ANG. radius. 9. The method of claim 8, wherein at least one of the substitutions within the 7 .ANG. radius of the substrate binding region that leads to the reduced positive charge or increased negative charge is at a position selected from 99, 154, or 211 or combinations thereof, relative to the unmodified Bacillus lentus alkaline protease. 10. The method of claim 9, wherein the mutant protease has one or more substitutions selected from the group consisting of R99G, R99S, R99A, L211D, L211E, S154D, and S154E. 11. The method of claim 8 wherein the mutant protease is a protease M130, or a protease M131, or both a protease M130 and a protease M131, and wherein said mutant protease is modified by one or more amino acid substitutions in its amino acid sequence at a position that lies within a 7 .ANG. radius of the substrate binding region of the unmodified Bacillus lentus alkaline protease, wherein said one or more amino acid substitutions leads to a reduced positive charge or an increased negative charge. 12. The method of claim 11, wherein the mutant protease has one or more amino acid substitutions at a position selected from 99, 154, or 211 or combinations thereof relative to the unmodified Bacillus lentus alkaline protease. 13. A method of preparing a cosmetic composition, comprising mixing at least one mutant Bacillus lentus alkaline protease with at least one cosmetic material compatible with the mutant protease, wherein the mutant protease comprises a Bacillus lentus alkaline protease having one or more amino acid substitutions in its amino acid sequence, wherein at least one of the substitutions (i) occurs at a position lying within a 7 .ANG. radius of the substrate binding region of the unmodified Bacillus lentus alkaline protease, (ii) leads to a reduced positive charge or an increased negative charge and (iii) results in the mutant protease having reduced tissue surface irritation relative to the modified or unmodified Bacillus lentus alkaline protease not having these substitutions within the 7 .ANG. radius. 14. The method of claim 13 wherein at least one of