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The invention relates to reverse focusing and methods and systems which employ reverse focusing. In a particular example, an automated focusing microscope uses one or more reverse focusing steps to acquire a focused image. Computer programs having instructions for instructing a computer to acquire a

The invention relates to reverse focusing and methods and systems which employ reverse focusing. In a particular example, an automated focusing microscope uses one or more reverse focusing steps to acquire a focused image. Computer programs having instructions for instructing a computer to acquire a focused image by reverse focusing are also included.

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The invention relates to reverse focusing and methods and systems which employ reverse focusing. In a particular example, an automated focusing microscope uses one or more reverse focusing steps to acquire a focused image. Computer programs having instructions for instructing a computer to acquire a

The invention relates to reverse focusing and methods and systems which employ reverse focusing. In a particular example, an automated focusing microscope uses one or more reverse focusing steps to acquire a focused image. Computer programs having instructions for instructing a computer to acquire a focused image by reverse focusing are also included. laim 18, said seed comprising said expression cassette. 20. A method for expressing a nucleotide sequence in a plant, said method comprising, stably incorporating into the genome of a plant cell an expression cassette comprising a promoter operably linked to a nucleotide sequence, wherein said promoter is the promoter of claim 15. 21. The method of claim 20, wherein said nucleotide sequence is selectively expressed in the root. 22. A plant cell having stably incorporated into its genome an expression cassette comprising a promoter operably linked to a nucleotide sequence, wherein said promoter is the promoter of claim 15.