초록 ▼

Viable biological material is cryogenically preserved (cryopreservation) by preparing the material for freezing, immersing the material in a tank of cooling fluid, and circulating the cooling fluid past the material at a substantially constant predetermined velocity and temperature to freeze the mat

Viable biological material is cryogenically preserved (cryopreservation) by preparing the material for freezing, immersing the material in a tank of cooling fluid, and circulating the cooling fluid past the material at a substantially constant predetermined velocity and temperature to freeze the material. A method according to the present invention freezes the biologic material quickly enough to avoid the formation of ice crystals within cell structures (vitrification). The temperature of the cooling fluid is preferably between -20° C. and -30° C., which is warm enough to minimize the formation of stress fractures in cell membranes due to thermal changes. Cells frozen using a method according to the present invention have been shown to have approximately an 80 percent survival rate, which is significantly higher than other cryopreservation methods.

대표청구항 ▼

Viable biological material is cryogenically preserved (cryopreservation) by preparing the material for freezing, immersing the material in a tank of cooling fluid, and circulating the cooling fluid past the material at a substantially constant predetermined velocity and temperature to freeze the mat

Viable biological material is cryogenically preserved (cryopreservation) by preparing the material for freezing, immersing the material in a tank of cooling fluid, and circulating the cooling fluid past the material at a substantially constant predetermined velocity and temperature to freeze the material. A method according to the present invention freezes the biologic material quickly enough to avoid the formation of ice crystals within cell structures (vitrification). The temperature of the cooling fluid is preferably between -20° C. and -30° C., which is warm enough to minimize the formation of stress fractures in cell membranes due to thermal changes. Cells frozen using a method according to the present invention have been shown to have approximately an 80 percent survival rate, which is significantly higher than other cryopreservation methods. without risk of sag, deformation, separation of blow-out of the corner assembly, and further comprising a removable supporting means disposed vertically within the corner assembly to provide vertical support to the corner assemblies, a horizontal plate being formed at a base of the supporting means to anchor the corner assembly to a foundation surface and to act as a pivot point for minor adjustments. 11. A corner assembly for concrete form panels comprising: a pair of rigid members, each member having an inner face and a bend therein forming a selected dihedral angle, the rigid members being spaced apart by a selected distance, wherein the first rigid member is formed from a first submember and a second submember, each submember having a longer side and a shorter side, there being a selected acute angle between the longer side and the shorter side, the shorter sides of the submembers abutting and connecting to one another such that the longer sides form an outer corner, a first concrete panel form having a side formed at the selected acute angle being disposed adjacent to the selected acute angle of the