초록 ▼

A method for fluorescence probing of particles flowing in a fluid includes defining a trigger volume in the fluid by intersecting a plurality of substantially orthogonally aimed trigger laser beams, each of a different wavelength, detecting light scattered from the vicinity of the trigger volume by

A method for fluorescence probing of particles flowing in a fluid includes defining a trigger volume in the fluid by intersecting a plurality of substantially orthogonally aimed trigger laser beams, each of a different wavelength, detecting light scattered from the vicinity of the trigger volume by a plurality of particle detectors each sensitive to a wavelength corresponding to the wavelength of a trigger laser beam, probing the particles with a pulsed laser triggered by the particle detectors, collecting fluorescence emitted from the particle in a detection volume and focusing it in a detection region, detecting the fluorescence focused in the detection region. The invention also includes devices for carrying out the foregoing method.

대표청구항 ▼

A method for fluorescence probing of particles flowing in a fluid includes defining a trigger volume in the fluid by intersecting a plurality of substantially orthogonally aimed trigger laser beams, each of a different wavelength, detecting light scattered from the vicinity of the trigger volume by

A method for fluorescence probing of particles flowing in a fluid includes defining a trigger volume in the fluid by intersecting a plurality of substantially orthogonally aimed trigger laser beams, each of a different wavelength, detecting light scattered from the vicinity of the trigger volume by a plurality of particle detectors each sensitive to a wavelength corresponding to the wavelength of a trigger laser beam, probing the particles with a pulsed laser triggered by the particle detectors, collecting fluorescence emitted from the particle in a detection volume and focusing it in a detection region, detecting the fluorescence focused in the detection region. The invention also includes devices for carrying out the foregoing method. lenn, 361/783; US-6020629, 20000200, Farnworth et al., 257/868; US-6025213, 20000200, Nemoto et al., 438/122; US-6025767, 20000200, Kellam et al., 335/128