초록 ▼

In a competitive receptor binding assay for detecting TSH-receptor auto-antibodies in a biological sample, the sample is reacted in a reaction mixture which contains (i) a TSH-receptor or TSH-receptor preparation; (ii) a primary competitor, for example labelled TSH; and (iii) an agent for separating

In a competitive receptor binding assay for detecting TSH-receptor auto-antibodies in a biological sample, the sample is reacted in a reaction mixture which contains (i) a TSH-receptor or TSH-receptor preparation; (ii) a primary competitor, for example labelled TSH; and (iii) an agent for separating a complex composed of the TSH-receptor and the elements bound thereto of the reaction mixture from the liquid phase. According to the invention, the reaction is carried out in the presence of at least one monoclonal or polyclonal antibody specific against a partial peptide sequence of the TSH eceptor. This specific antibody is used to immobilize a complex of TSH-receptor and primary competitor and/or as secondary competitor for another part of the TSH-receptor auto-antibodies expected in a sample. The primary or secondary competitors are or can be selectively labelled.

대표청구항 ▼

In a competitive receptor binding assay for detecting TSH-receptor auto-antibodies in a biological sample, the sample is reacted in a reaction mixture which contains (i) a TSH-receptor or TSH-receptor preparation; (ii) a primary competitor, for example labelled TSH; and (iii) an agent for separating

In a competitive receptor binding assay for detecting TSH-receptor auto-antibodies in a biological sample, the sample is reacted in a reaction mixture which contains (i) a TSH-receptor or TSH-receptor preparation; (ii) a primary competitor, for example labelled TSH; and (iii) an agent for separating a complex composed of the TSH-receptor and the elements bound thereto of the reaction mixture from the liquid phase. According to the invention, the reaction is carried out in the presence of at least one monoclonal or polyclonal antibody specific against a partial peptide sequence of the TSH eceptor. This specific antibody is used to immobilize a complex of TSH-receptor and primary competitor and/or as secondary competitor for another part of the TSH-receptor auto-antibodies expected in a sample. The primary or secondary competitors are or can be selectively labelled. 1990). Schwab et al., 1989, 1989, Oncogene 4:139-144. Sczakiel G. et al., Bioch. Biophys. Res. Comm. 169(2):643-651 (1990). Shen et al., Science 232: 643-645 (1986). Shih et al., 1979, Proc. Natl. Acad Sci. USA 76:5714-5718. Solomon et al., 1991, Science, 254: 1153-1160. Trent et al., 1989, Cancer Res. 49: 420-423. Trono et al., Cell 59:113-120 (1989). Tsai-Pflugfleder et al. Proc. Natl. Acad. Sci. USA 85:7177-7181 (1988). Uhlmann and Peyman Chemical Reviews 90: 543-584 (1990). Vale, 1987, Ann. Rev. Cell Biol. 3:347-378. van der Krol et al., Biotechniques 6:958-976 (1988). Viskochil et al, 1990, Cell 62: 187-192. Vogelstein et al., 1988, N. Engl. J. Med. 319:525-532. Vogelstein et al., 1993 vol. 9 No. 4 pp. 138-141. Weinberg 1991, Science 254:1138-1146. Weinberger et al., 1986, Nature 324: 641-646. Wolos et al., 1993, J. Immunol, 150:3264-3273. Xu et al., 1990, Cell 62: 599-608.