IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0451930
(1999-12-01)
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발명자
/ 주소 |
- Zarling, David A.
- Rossi, Michel J.
- Peppers, Norman A.
- Kane, James
- Faris, Gregory W.
- Dyer, Mark J.
- Ng, Steve Y.
- Schneider, Luke V.
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출원인 / 주소 |
|
대리인 / 주소 |
Morgan, Lewis & Bockius LLP
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인용정보 |
피인용 횟수 :
114 인용 특허 :
51 |
초록
▼
The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits
The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. The methods, compositions, and apparatus are suitable for the sensitive detection of multiple analytes and for various clinical and environmental sampling techniques.
대표청구항
▼
The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits
The invention provides methods, compositions, and apparatus for performing sensitive detection of analytes, such as biological macromolecules and other analytes, by labeling a probe molecule with an up-converting label. The up-converting label absorbs radiation from an illumination source and emits radiation at one or more higher frequencies, providing enhanced signal-to-noise ratio and the essential elimination of background sample autofluorescence. 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Kolter (1992). "Life and Death in Stationary Phase" ASM news 58:75-79. Kruse, P.F. and Patterson, M.K., eds., (1973) Tissue Culture, Method and Applications, Academic Press: New York pp. v-xiii (Table of Contents). Laemmli, U.K. (1970). "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4" Nature 227(259):680-685. Lange, R. et al. (1991). "Identification of a Central Regulator of Stationary-Phase Gene Expression in Escherichia Coli" Mol. Microbiol. 5(1):49-59. Latimer, L.J. et al. (1995). "Specificity of Monoclonal Antibodies Produced Against Phosphorothioate and Ribo Modified DNAs" Mol. Immunol. 32(14-15): 1057-1064. Leckie, M.P. et al. (1983). "Regulation of Bacterial Glycogen Synthesis. Stimulation of Glycogen Synthesis by Endogenous and Exogenous Cyclic Adenosine 3':5'-Monophosphate in Escherichia Coli and the Requirement for a Functional CRP Gene" J. Biol. Chem. 258(6):3813-3824. Leckie, M.P. et al. (1985). "Independence of Cyclic AMP and relA Gene Stimulation of Glycogen Synthesis in Intact Escherichia Coli Cells" J. Bacteriol. 161(1):133-140. Liu, M et al. (1997). "The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in Escherichia coli." J. Biol. Chem. 272:17502-17510. Liu, M.Y. et al. (1995). "The Product of the Pleiotropic Escherichia Coli Gene csrA Modulates Glycogen Biosynthesis Via Effects on mRNA Stability" J. Bacteriol. 177(10):2663-2672. Matin, A. (1991). "The Molecular Basis of Carbon-Starvation-Induced General Resistance in Escherichia Coli" Mol. Microbiol. 5(1):3-10. Matin, A. et al. (1989). "Genetic B
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