Compositions and methods for the degradation of compounds contained in a liquid or solid waste stream are provided. Genes encoding toluene-degrading enzymes are also provided. The enzymes have homology to the E. coli pyruvate formate lyase and pyruvate formate lyase activator.
대표청구항▼
1. A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of: a) providing i) a liquid or solid waste source comprising toluene, ii) a reaction containing means, iii) a functional, cell-free pyruvate formate lyase obtained from a toluene-degrading ba
1. A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of: a) providing i) a liquid or solid waste source comprising toluene, ii) a reaction containing means, iii) a functional, cell-free pyruvate formate lyase obtained from a toluene-degrading bacterium and iv) a functional, cell-free pyruvate formate lyase activating enzyme of a toluene-degrading bacterium; and b) reacting said functional, cell-free pyruvate fornate lyase of a toluene-degrading bacterium and said functional, cell-free pyruvate formate lyase activating enzyme of a toluene-degrading bacterium with said liquid or solid waste source in said reaction containing means under conditions such that toluene is degraded. 2. The method of claim 1, wherein said functional, cell-free pyruvate formate lyase obtained from a toluene-degrading bacterium and said functional, cell-free pyruvate formate lyase activating enzyme of a toluene-degrading bacterium are derived from an organism selected from the group consisting of Thauera aromatica, Xanthomonas maltophilia, Geobacter metallireducens, and Azoarcus tolulyticus. 3. The method of claim 1, wherein said functional, cell-free pyruvate formate lyase obtained from a toluene-degrading bacterium has the amino acid sequence selected from the group consisting of SEQ ID NO: 16, SEQ ID NO: 33, SEQ ID NO: 34 and SEQ ID NO: 35. 4. The method of claim 1, wherein said functional, cell-free pyruvate formate lyase activating enzyme obtained from a toluene-degrading bacterium has the amino acid sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 37 and SEQ ID NO: 38. 5. The method of claim 1, wherein said reaction containing means is a bioreactor. 6. A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of: a) providing i) a liquid or solid waste source comprising toluene, and ii) a functional, cell-free pyruvate formate lyase tutD homologue obtained from a toluene-degrading bacterium selected from the group consisting of Thauera aromatics, Xanthomonas maltophilia, Geobacter metallireducens, and Azoarcus tolulyticus; b) reacting said functional, cell-free pyruvate formate lyase homologue with said liquid or solid waste source under conditions such that toluene is degraded. 7. The method of claim 6, further comprising the step of reacting a functional, cell-free pyruvate fornate lyase activating homologue with said waste source. 8. A method of degrading compounds contained in a liquid or solid waste source, comprising the steps of: a) providing i) a liquid or solid waste source comprising toluene, ii) a reaction containing means, iii) a functional, cell-free pyruvate fornate lyase having the amino acid sequence of SEQ ID NO: 16 and iv) a functional, cell-free pyruvate formate lyase activating enzyme having the amino acid sequence of SEQ ID NO: 18; and b) reacting said functional, cell-free pyruvate formate lyase and said functional, cell-free pyruvate formate lyase activating enzyme with said liquid or solid waste source in said reaction containing means under conditions such that toluene is degraded.
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이 특허에 인용된 특허 (27)
Bryant Curtis W. (Tucson AZ) Barkley William A. (Maple Valley WA), Aerobic biological dehalogenation reactor.
Yen Kwang-Mu (Thousand Oaks CA) Blatt Lawrence M. (West Hills CA) Karl Michael R. (Port Hueneme CA), Bioconversions catalyzed by the toluene monooxygenase of Pseudomanas mendocina KR-1.
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Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
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