초록 ▼

Mixtures containing a biological macromolecule, such as a nucleic acid molecule or a polypeptide, and a liquid matrix, which absorbs infrared (IR) radiation, are provided. These mixtures are useful for analysis of the biological macromolecule by IR matrix assisted laser desorption/ionization (IR-MAL

Mixtures containing a biological macromolecule, such as a nucleic acid molecule or a polypeptide, and a liquid matrix, which absorbs infrared (IR) radiation, are provided. These mixtures are useful for analysis of the biological macromolecule by IR matrix assisted laser desorption/ionization (IR-MALDI) mass spectrometry. Also provided are processes for analyzing a biological macromolecule using IR-MALDI mass spectrometry. For example, processes for detecting the presence or identity of a biological macromolecule in a sample, or for sequencing a biological macromolecule are provided.

대표청구항 ▼

Mixtures containing a biological macromolecule, such as a nucleic acid molecule or a polypeptide, and a liquid matrix, which absorbs infrared (IR) radiation, are provided. These mixtures are useful for analysis of the biological macromolecule by IR matrix assisted laser desorption/ionization (IR-MAL

Mixtures containing a biological macromolecule, such as a nucleic acid molecule or a polypeptide, and a liquid matrix, which absorbs infrared (IR) radiation, are provided. These mixtures are useful for analysis of the biological macromolecule by IR matrix assisted laser desorption/ionization (IR-MALDI) mass spectrometry. Also provided are processes for analyzing a biological macromolecule using IR-MALDI mass spectrometry. For example, processes for detecting the presence or identity of a biological macromolecule in a sample, or for sequencing a biological macromolecule are provided. 61 (1989). J. Almeida et al., Morphology of the GB hepatitis agent, Nature vol. 261: p. 608-609 (1976). F. Deinhardt et al., Studies on the Transmission of Human Viral Heptitis to Marmoset Monkeys, Journal of Experimental Medicine vol. 125: p. 673-688, Plate 81-86 (1966). J. Dienstag, Non-A, Non-B Hepatitis. II. 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Alter et al., Clinical and Serological Analysis of Transfusion-Associated Hepatitis, The Lancet: p. 838-841 (1975). S. Feinstone et al., Transfusion-Associated Hepatitis Not Due To Viral Hepatitis Type A or B, The New England Journal of Medicine vol. 292 No. 15: p. 767-770 (1975). J. Simons et al., Identification of two flavivirus-like genomes in the GB Hepatitis agent, Proc. Natl. Acad. Sci. USA vol. 92: 3401-3405 (1995). J. Simons et al. , Isolation of novel virus-like sequences associated with human hepatitis, Nature Medicine vol. 1 No. 6: p. 564-568 (1995). G. Schlauder et al., Molecular and Serologic Analysis in the Transmission of the GB Hepatitis Agents, Journal of Medical Virology vol. 46: p. 81-90 (1995). M. Yoshiba et al., Detection of the GBV-C hepatitis virus genome in serum from patients with fulminant hepatitis of unknown aetiology, The Lancet vol. 346: p. 1131-1132 (1995). J. 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Muerhoff et al., Journal of Virological Methods, vol. 62, No. 1, pp. 55-62 (1996). S. Vijayasarathy, Nucleic Acids Research, vol. 18, pp. 2967-2975 (1990). S. Tijssen, "Practice and Theory of Enzyme Immunoassays", Elsevier, Amsterdam, pp. 333-340 (1985). A. Takamizawa et al., Journal of Virology, vol. 65, No. 3, pp. 1105-1113 (1991). e method of claim 12, wherein the released compound is detected via mass spectroscopy. 21. The method of claim 12, wherein the number of compounds is less than or equal to the number of viral sites. 22. The method of claim 12, wherein the number of compounds is in excess of the number of viral sites.