초록 ▼

The invention provides fibrin-based, biocompatible materials useful in promoting cell growth, wound healing, and tissue regeneration. These materials are provided as part of several cell and tissue scaffolding structures that provide particular application for use in wound-healing and tissue regener

The invention provides fibrin-based, biocompatible materials useful in promoting cell growth, wound healing, and tissue regeneration. These materials are provided as part of several cell and tissue scaffolding structures that provide particular application for use in wound-healing and tissue regenerating. Methods for preparing these compositions and using them are also disclosed as part of the invention. A variety of peptides may be used in conjunction with the practice of the invention, in particular, the peptide IKVAV, and variants thereof. Generally, the compositions may be described as comprising a protein network (e.g., fibrin) and a peptide having an amino acid sequence that comprises a transglutaminase substrate domain (e.g., a factor XIIIa substrate domain) and a bioactive factor (e.g., a peptide or protein, such as a polypeptide growth factor), the peptide being covalently bound to the protein network. Other applications of the technology include their use on implantable devices (e.g., vascular graphs), tissue and cell scaffolding. Other applications include use in surgical adhesive or sealant, as well as in peripheral nerve regeneration and angiogenesis.

대표청구항 ▼

1. A composition comprising a fibrin network and a peptide having an amino acid sequence that comprises one transglutaminase substrate domain and a bioactive factor, wherein the peptide is covalently bound to the fibrin network by the transglutaminase substrate domain. 2. The composition of claim 1

1. A composition comprising a fibrin network and a peptide having an amino acid sequence that comprises one transglutaminase substrate domain and a bioactive factor, wherein the peptide is covalently bound to the fibrin network by the transglutaminase substrate domain. 2. The composition of claim 1 wherein the transglutaminase substrate domain is a factor XIIIa substrate domain. 3. The composition of claim 2 wherein the factor XIIIa substrate domain comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and combinations and bioactive fragments thereof. 4. The composition of claim 2 wherein the factor XIIIa substrate domain comprises an amino acid sequence of SEQ ID NO: 15. 5. The composition of claim 1 wherein the bioactive factor is a peptide. 6. The composition of claim 1 wherein the wherein the bioactive factor comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, and combinations and bioactive fragments thereof. 7. The composition of claim 1 wherein the bioactive factor is a protein. 8. The composition of claim 1 wherein the bioactive factor is a polypeptide growth factor. 9. A method for promoting cell growth or tissue regeneration comprising: covalently attaching a bioactive factor to a transglutaminase substrate to form a bioactive complex molecule; covalently coupling the bioactive complex molecule to a fibrin network to form a treated fibrin substrate; and exposing the treated fibrin substrate to a composition comprising cells or tissue to promote cell growth or tissue regeneration. 10. The method of claim 9 wherein the composition comprising cells comprises nerve cells. 11. The method of claim 9 wherein the composition comprising cells comprises skin cells. 12. The method of claim 9 wherein the transglutaminase substrate is a factor XIIIa substrate. 13. The method of claim 12 wherein the factor XIIIa substrate comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and combinations and bioactive fragments thereof. 14. The method of claim 13 wherein the factor XIIIa substrate comprises an amino acid sequence of SEQ ID NO: 15. 15. The method of claim 9 wherein the bioactive factor is a peptide. 16. The method of claim 9 wherein the bioactive factor is a protein. 17. The method of claim 9 wherein the bioactive factor is a polypeptide growth factor. 18. The method of claim 9 wherein the bioactive factor comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, and combinations and bioactive fragments thereof.