초록 ▼

A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stab

A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.

대표청구항 ▼

A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stab

A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling. Carbohydrate Antigen", Anticancer Research 10: 1579-1586. Hanai et al., "Generation of Monoclonal Antibodies Against Human Lung Squamous Cell Carcinoma and Adenocarcinoma Using Mice Rendered Tolerant to Normal Human Lung", Cancer Research 46, 4438-4443, Sep. 1986. Shitara et al., "Application of Anti Lung Adenocarcinoma Monoclonal Antibody Recognizing Cytokeratin-Like Cytoplasmic Antigen for Tumor Diagnosis", Anticancer Research 12: 1121-1130 (1992). Bird, et al, "Single-Chain Antigen-Binding Proteins", Science, vol. 242, Oct. 1988, pp. 423-426. Mitola et al, "Tat-Human Immunodeficiency Virus-1 Induces Human Monocyte Chemotaxis by Activation of Vascular Endothelial Growth Factor Receptor-1". Blood, vol. 90, No. 4 (Aug. 15, 1997, pp 1365-1372). Webber et al, "Preparation and Characterization of a Disulfide-Stabilized Fv Fragment of the Anti-TAC Antibody: Comparison with its Single-Chain Analog", Molecular Immunology, vol. 32, No. 4, pp. 249-258, 1995. Shibuya, "Role of VEGF-FLT Receptor System in Normal and Tumor Angiogenesis", Advances in Cancer Research, vol. 67, Institute of Medical Science, University of Tokyo, Copyright 1995, pp. 281-316. Davis-Smyth et al, EMBO Journal, vol. 15, No. 18, 1996, pp. 4919-4927. Tanaka, et al., Characterization of the Extracellular Domain in Vascular Endothelial Growth Factor Receptor-1 (Flt-1 Tyrosine Kinase), Sep. 1997, Jpn J. Cancer Research, 88, pp. 867-876. Wiesmann, et al., Crystal Structure at 1.7 .ANG. Resolution of VEGF in Complex with Domain 2 of the Flt-1 Receptor, Nov. 28, 1997, Cell, vol. 91, pp. 694-704.