초록 ▼

A method for testing gas detection instruments includes providing at least two reagents, immobilizing at least one of the reagents into a matrix material, heating the matrix material until the matrix permits movement of the reagent and generating a gas responsive to chemical reaction between the rea

A method for testing gas detection instruments includes providing at least two reagents, immobilizing at least one of the reagents into a matrix material, heating the matrix material until the matrix permits movement of the reagent and generating a gas responsive to chemical reaction between the reagents. The gas is introduced into the sensor portion of the gas detection instrument to test the same. The reagents may each be immobilized on the matrix material with the heating serving to soften or melt the matrix material to permit chemical interaction. In a preferred embodiment, the heating is effected at about 90 to 150° C. The method may be employed to generate carbon monoxide or other gases of interest. Corresponding apparatus is provided. The apparatus may be structured to be inserted into or receive the gas detection instrument or have its output in communication therewith.

대표청구항 ▼

A method for testing gas detection instruments includes providing at least two reagents, immobilizing at least one of the reagents into a matrix material, heating the matrix material until the matrix permits movement of the reagent and generating a gas responsive to chemical reaction between the rea

A method for testing gas detection instruments includes providing at least two reagents, immobilizing at least one of the reagents into a matrix material, heating the matrix material until the matrix permits movement of the reagent and generating a gas responsive to chemical reaction between the reagents. The gas is introduced into the sensor portion of the gas detection instrument to test the same. The reagents may each be immobilized on the matrix material with the heating serving to soften or melt the matrix material to permit chemical interaction. In a preferred embodiment, the heating is effected at about 90 to 150° C. The method may be employed to generate carbon monoxide or other gases of interest. Corresponding apparatus is provided. The apparatus may be structured to be inserted into or receive the gas detection instrument or have its output in communication therewith. . 9. The method of claim 2, wherein (i) attB comprises a first DNA sequence (attB5'), a bacterial core region, and a second DNA sequence (attB3') in the order attB5'-bacterial core region-attB3', (ii) attP comprises a first DNA sequence (attP5'), a phage core region, and a second DNA sequence (attP3') in the order attP5'-phage core region-attP3', and (iii) the recombinase mediates production of recombination-product sites that can no longer act as a substrate for the recombinase, said recombination-product sites comprising the order attB5'-(recombination-product site)-attP3' and attP5'-(recombination-product site)-attB3'. 10. The method of claim 9, wherein (i) said second recombination site is a pseudo-attP site, and said second recombination site comprises a first DNA sequence (attT5'), a core region B, and a second DNA sequence (attT3') in the order attT5'-core region B-attT3', (ii) said first recombination site is an attB site comprising attB5'-bacterial core region-attB3', in the order recited and (iii) the recombinase mediates production of recombination-product sites that can no longer act as a substrate for the recombinase, said recombination-product sites comprising the order attT5'-(recombination-product site)-attB3'{polynucleotide of interest}attB5'-(recombination-product site)-attT3'. 11. The method of claim 9, wherein (i) said second recombination site is a pseudo-attB site, and said second recombination site comprises a first DNA sequence (attT5'), a core region B, and a second DNA sequence (attT3') in the order attT5'-core region B-attT3', (ii) said first recombination site is an attP site comprising attP5'-phage core region-attP3', in the order recited and (iii) the recombinase mediates production of recombination-product sites that can no longer act as a substrate for the recombinase, said recombination-product sites comprising the order attT5'-(recombination-product site)-attP3'{polynucleotide of interest}attP5 '-(recombination-product site)attT3'. 12. The method of claim 1, wherein said circular targeting construct further comprises a bacterial origin of replication. 13. The method of claim 1, wherein said circular targeting construct further comprises a selectable marker. 14. The method of claim 13, wherein said selectable marker provides for either positive or negative selection. 15. The method of claim 1, wherein said polynucleotide sequence of interest comprises a promoter sequence. 16. The method of claim 1, wherein said polynucleotide sequence of interest comprises at least one expression cassette. 17. The method of claim 16, wherein said expression cassette of said polynucleotide sequence of interest comprises a promoter operably linked to a polynucleotide sequence that encodes a product. 18. The method of claim 17, wherein said product is an RNA molecule. 19. The method of claim 17, wherein said product is a polypeptide. 20. The method of claim 1, wherein the expression cassette comprising a polynucleotide encoding the site-specific recombinase is carried on a transient expression vector. 21. The method of claim 1, wherein said expression cassette comprising a polynucleotide encoding the site-specific recombinase is introduced into the isolated eukaryotic cell before introducing the circular targeting construct. 22. The method of claim 1, wherein said expression cassette comprising a polynucleotide encoding the site-specific recombinase is introduced into the isolated eukaryotic cell concurrently with introducing the circular targeting construct. 23. The method of claim 1, wherein said expression cassette comprising a polynucleotide encoding the site-specific recombinase is introduced into the isolated eukaryotic cell after introducing the circular targeting construct. 24. A vector for site-specific integration of a polynucleotide sequence into the genome of an isolated eucaryotic cell, said vector comprising, (i) a circular backbone vector, (ii) a polynucleotide of interest operably linked to a e