초록 ▼

Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a mat

Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.

대표청구항 ▼

Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a mat

Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided. ith an antigen, hybridizing the labeled plurality of methylated nucleic acid segments to said array and detecting said antigen with a molecule which binds said antigen. 17. The method of claim 9, wherein said solid support comprises a hybridization filter. 18. The method of claim 7, wherein said detecting comprises radioactively labeling said plurality of methylated nucleic acid segments and hybridizing the labeled plurality of methylated nucleic acid segments to said array. 19. The method of claim 7, wherein said array comprises a plurality of DNA pools, said pools comprising the nucleic acid sequences of at least a first and a second clone comprising genomic DNA from said selected organism. 20. The method of claim 2, wherein said contacting is further defined as comprising: a) obtaining a second sample of genomic DNA from said selected organism; b) contacting said second sample of genomic DNA with an isoschizomer of said methylation sensitive restriction endonuclease, wherein said isoschizomer is not methylation sensitive; c) resolving separately said first and said second samples of genomic DNA following said contacting with said isoschizomer and said methylation sensitive restriction endonuclease; and d) selecting a plurality of methylated nucleic acid segments from at least a first nucleic acid fraction present in said first sample of genomic DNA and not present in said second sample of genomic DNA. 21. The method of claim 20, further defined as comprising contacting said second sample of genomic DNA with said methylation sensitive restriction endonuclease. 22. The method of claim 1, wherein said methylation sensitive restriction endonuclease is selected from the group consisting of AatII, AccIII, Acil, AfaI, Agel, AhaII, Alw261, Alw44I, ApaLI, ApyI, Ascl, Asp718I, AvaI, AvaII, Bme216I, BsaAI, BsaHI, BscFI, BsiMI, BsmA1, BsiEI, BsiWI, BsoFI, Bsp105I, Bsp119I, BspDI, BspEI, BspHI, BspKT6I, BspMII, BspRI, BspT104I, BsrFI, BssHII, BstBI, BstEIII, BstUI, BsuFI, BSUR1, CacI, CboI, CbrI, CceI, CHOI, ClaI, Csp68KII, Csp45I, CtyI, CviAI, CviSIII, DpnII, EagI, Ecl136II, Eco47I, Eco47III, EcoRII, EcoT221, EheI, Esp31, Friu4HI, FseI, FspI, Fsp4HI, GsaI, HaeII, HaeIII, Hgal, HhaI, HlriPlI, HpaII, HpyAIII, ItaI, KasI, Kpn2I, LlaAI, LlaKR2I, MboI, NMI, MluI, MmeII, MroI, MspI, MstII, MthTI, NaeI, NarI, NciAl, NdeII, NgoMIV, NgoPII, NgoS II, NIaIII, NlaIV, NotI, NruI, NspV PmeI, PmlI, Psp14061, PvuI, RalF40I, RsaI, RspXI, RsrII, SacII, Sall, Sau3AI, SeXAl, SfoI, SfuI, SmaI, SriaB1, SolI, SpoI, SspRFI, Sth368I, Tail, TaqI, TflI, TthHB81, VpaK11BI, and XhoI. 23. The method of claim 20, wherein said isoschizomer is selected from the group consisting of AccIII, AflI, Alw26I, Alw44I, AmaI, AorI, ApaLI, ApyI, AspMDI, BanlFI, BamHI, BamKI, BanII, BbeI, BbsI, Bce2431, Bfi57I, BpmI, BsaBC31, BsaHI, BsaJI, BsaWI, BshGI, BsiLI, BSMI, BsmAI, BSOBl, BsoFI, Bsp1221, Bspl2861, Bsp1431, Bsp14311, Bsp2095I, Bsp491, Bsp511, Bsp521, Bsp54I, Bsp561, Bsp571, Bsp58I, Bsp59I, Bsp601, Bsp61I, Bsp641, Bsp65I, Bsp66I, Bsp67I, Bsp72I, Bsp91I, BspAI, BspEI, BspFI, BspJ641, BspLI, BspMI, BspMII, BsrBI, BsrPII, BstI, BSt2UI, BStEIl, BstNI, BstOI, BstYI, Bsu36I, BtcI, BuaI, CbiI, CceI, CcyI, CpfI, Csp51, Csp61, CViAll, CviQI, Eam1105I, Earl, Eco0I09I, EcoRl, EcoRV, EheI, ESaBC4I, FnuEI, Fokl, HaeIII, HgiAI, HpaII, HphI, ItaI, Kasl, KpnI, Kpn2I, Kzo9I, MabI, MboI, MroI, MspI, MspBI, MssI, MvaI, NarI, NdeII, NgoPII, NsiI, PaeR7I, PagI, Pei94031I, PfaI, PmeI, PspGI, PsuI, SacI, SalDI, Sau3AI, SauMI, Sbo131, SfaNI, SfuI, SphI, Sth3681, TaqI, TaqXI, TfiI, Tth111I, XhoII, XmaI, and ZanI. 24. The method of claim 2, wherein the resistance to cleavage with said methylation sensitive restriction endonuclease is determined by a method comprising measuring the length of said methylated nucleic acid segments following said contacting. 25. The method of claim 24, wherein the average length of said plurality of methylated nucleic acid segments is at least 3 kb. 26. The meth