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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0911341 (2001-07-23) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 333 인용 특허 : 10 |
The invention relates generally to methods, systems, and devices for measuring the concentration of target analytes present in a biological system using a series of measurements obtained from a monitoring system and a Mixtures of Experts (MOE) algorithm. In one embodiment, the present invention desc
The invention relates generally to methods, systems, and devices for measuring the concentration of target analytes present in a biological system using a series of measurements obtained from a monitoring system and a Mixtures of Experts (MOE) algorithm. In one embodiment, the present invention describes a method for measuring blood glucose in a subject.
The invention relates generally to methods, systems, and devices for measuring the concentration of target analytes present in a biological system using a series of measurements obtained from a monitoring system and a Mixtures of Experts (MOE) algorithm. In one embodiment, the present invention desc
The invention relates generally to methods, systems, and devices for measuring the concentration of target analytes present in a biological system using a series of measurements obtained from a monitoring system and a Mixtures of Experts (MOE) algorithm. In one embodiment, the present invention describes a method for measuring blood glucose in a subject. working heart is a mammalian heart. 20. The method of claim 19, wherein the isolated working heart is a rabbit heart, a rat heart or a mouse heart. 21. The method of claim 18, wherein the isolated working heart is subjected to a trauma or a drug. 22. The method of claim 21, wherein the trauma is ischemia. 23. The method of claim 18, wherein the isolated working heart is reperfused. 24. The method of claim 18, wherein glycolysis is measured by quantitative collection of 3H2O production from glucose. 25. The method of claim 18, wherein glycolysis is measured by quantitative collection of 3H2O production from glucose, while simultaneously measuring glucose or lactate oxidation. 26. The method of claim 18, wherein glucose oxidation is measured by quantitative collection of 14CO2from glucose. 27. The method of claim 18, wherein glucose oxidation is measured by quantitative collection of 14CO2from glucose, while simultaneously measuring palmitate oxidation. 28. The method of claim 18, wherein glycogen turnover is measured by simultaneous quantitative collection of 14CO2from exogenous glucose and 3H2O from endogenous glycogen labeled with glucose. 29. The method of claim 18, wherein glycogen turnover is measured by simultaneous quantitative collection of 3H2O from exogenous glucose and 14CO2from endogenous glycogen labeled with glucose. 30. A method for measuring complete energy metabolism in a working heart comprising the following steps (a) providing an isolated working heart; (b) measuring fatty acid oxidation and lactate oxidation in the working heart simultaneously; and (c) measuring glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover in the working heart, wherein the glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover are measured simultaneously, or, serially, wherein step (c) can be performed during (b), thereby measuring complete energy metabolism in the working heart. 31. The method of claim 30, wherein the isolated working heart is a mammalian heart. 32. The method of claim 31, wherein the isolated working heart is a rabbit heart, a rat heart or a mouse heart. 33. The method of claim 30, wherein the isolated working heart is subjected to a trauma or a drug. 34. The method of claim 33, wherein the trauma is ischemia. 35. The method of claim 30, wherein the isolated working heart is reperfused. 36. A method for measuring exogenous energy metabolism during ischemia by a working heart comprising the following steps (a) providing an isolated ischemic working heart, wherein the ischemia is induced by perfusion at a low flow rate; (b) measuring fatty acid oxidation and lactate oxidation in the ischemic working heart simultaneously; and, (c) measuring glycolysis and glucose oxidation in the ischemic working heart, wherein the glycolysis and glucose oxidation are measured simultaneously, or, serially, wherein step (c) can be performed during or after step (b), thereby measuring exogenous energy metabolism in the working heart during ischemia. 37. The method of claim 36, wherein the isolated working heart is a rabbit heart, a rat heart or a mouse heart. 38. The method of claim 36, wherein the ischemia is induced by stopping perfusate flow through a left atrial inflow line and limiting flow throughout an aortic outflow line, thereby generating any range of aortic perfusion rates and any degree of ischemia. 39. The method of claim 36, wherein severe ischemia is induced by generating aortic perfusion rates ranging from about 0.5 ml/min to about 2 ml/min. 40. The method of claim 36, wherein moderate ischemia is induced by generating aortic perfusion rates from about 2 ml/min to about 5 ml/min. 41. The method of claim 36, wherein mild ischemia is induced by generating aortic perfusion rates from abou t 5 ml/min to about 15 ml/min. 42. A method for measuring overall energy metabolism in an ischemic working heart comprising the following steps (a) providing an isolated ischemic working heart, wherein the ischemia is induced by perfusion at a low flow rate; (b) measuring fatty acid oxidation and lactate oxidation in the ischemic working heart simultaneously; and (c) measuring glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover in the ischemic working heart, wherein the glycolysis, glucose oxidation, triacylglycerol turnover and glycogen are measured simultaneously, or, serially, wherein step (c) can be performed during step (b), thereby measuring overall energy metabolism in the ischemic working heart. 43. A method for measuring overall energy metabolism during reperfusion in an ischemic working heart comprising the following steps (a) providing an isolated reperfused working heart, wherein the reperfusion begins after ischemia is induced in the heart; (b) measuring fatty acid oxidation and lactate oxidation in the reperfused working heart simultaneously; followed by (c) measuring glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover in the reperfused working heart, wherein the glycolysis, glucose oxidation, triacylglycerol turnover and glycogen are measured simultaneously thereby measuring overall energy metabolism during reperfusion of the ischemic working heart. 44. A method for screening for compounds capable of modulating the energy metabolism of a working heart comprising the following steps (a) providing an isolated heart; (b) providing a test compound; (c) measuring the overall fatty acid endogenous and exogenous utilization of the heart as set forth in claim 1, the overall measure of glucose metabolism of the heart as set forth in claim 18, the complete energy metabolism of the heart as set forth in claim 30, the exogenous energy metabolism of the heart as set forth in claim 36, the overall energy metabolism of the heart during ischemia as set forth in claim 42, or, the overall energy metabolism during reperfusion of the working heart after inducement of ischemia as set forth in claim 43; (d) contacting the test compound to the heart; and (e) measuring the change in overall endogenous and exogenous fatty acid utilization of the heart as set forth in claim 1, the overall measure of glucose metabolism of the heart as set forth in claim 18, the complete energy metabolism of the heart as set forth in claim 30, the exogenous energy metabolism of the heart as set forth in claim 36, the overall energy metabolism of the heart during ischemia as set forth in claim 42, or, the overall energy metabolism during reperfusion of the working heart after inducement of ischemia as set forth in claim 43, wherein a change in the overall endogenous and exogenous fatty acid utilization, the overall measure of glucose metabolism, the complete energy metabolism, the exogenous energy metabolism, the overall energy metabolism of the heart during ischemia, or, the overall energy metabolism during reperfusion of the working heart after inducement of ischemia, after addition of the test compound indicates that the test compound can modulate the energy metabolism of a working heart. 45. A method for screening for compounds capable of modulating the energy metabolism of a working heart comprising the following steps (a) providing an isolated working heart; (b) contacting the isolated working heart with a test compound; (c) measuring the overall endogenous and exogenous fatty acid utilization of the heart as set forth in claim 1, the overall measure of glucose metabolism of the heart as set forth in claim 18, the complete energy metabolism of the heart as set forth in claim 30, the exogenous energy metabolism of the heart as set forth in claim 36, the overall energy metabolism of the heart during ischemia as set forth in claim 42, or, the overall energy metabolism duri ng reperfusion of the working heart after inducement of ischemia as set forth in claim 43; (d) contacting the isolated working heart without or with more or less of the test compound; and (e) measuring the change in overall fatty acid utilization of the heart as set forth in claim 1, the overall measure of glucose metabolism of the heart as set forth in claim 18, the complete energy metabolism of the heart as set forth in claim 30, the exogenous energy metabolism of the heart as set forth in claim 36, the overall energy metabolism of the heart during ischemia as set forth in claim 42, or, the overall energy metabolism during reperfusion of the working heart after inducement of ischemia as set forth in claim 43, wherein a change in the overall endogenous and exogenous fatty acid utilization, the overall measure of glucose metabolism, the complete energy metabolism, the exogenous energy metabolism, the overall energy metabolism of the heart during ischemia, or, the overall energy metabolism during reperfusion of the working heart after inducement of ischemia, after withdrawal or dilution or increase in amount of the test compound indicates that the test compound can modulate the energy metabolism of a working heart. 46. The method of claim 44 or claim 45, wherein the isolated working heart is a mammalian heart. 47. The method of claim 46, wherein the isolated working heart is a rabbit heart, a rat heart or a mouse heart. 48. The method of claim 44 or claim 45, wherein the test compound is administered to the heart by perfusion. 49. A method for measuring complete energy metabolism in a working heart comprising the following steps (a) providing an isolated working heart; (b) measuring fatty acid oxidation and lactate oxidation in the working heart simultaneously; and (c) measuring glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover in the working heart, wherein the glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover are measured simultaneously, or, serially, wherein step (c) can be performed after step (b), thereby measuring complete energy metabolism in the working heart. 50. A method for measuring overall energy metabolism in an ischemic working heart comprising the following steps (a) providing an isolated ischemic working heart, wherein the ischemia is induced by perfusion at a low flow rate; (b) measuring fatty acid oxidation and lactate oxidation in the ischemic working heart simultaneously; and (c) measuring glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover in the ischemic working heart, wherein the glycolysis, glucose oxidation, triacylglycerol turnover and glycogen are measured simultaneously, or, serially, wherein step (c) can be performed after step (b), thereby measuring overall energy metabolism in the ischemic working heart. 51. A method for measuring overall energy metabolism during reperfusion in an ischemic working heart comprising the following steps (a) providing an isolated reperfused working heart, wherein the reperfusion begins after ischemia is induced in the heart; (b) measuring fatty acid oxidation and lactate oxidation in the reperfused working heart simultaneously; followed by (c) measuring glycolysis, glucose oxidation, triacylglycerol turnover and glycogen turnover in the reperfused working heart, wherein the glycolysis, glucose oxidation, triacylglycerol turnover and glycogen are measured serially, thereby measuring overall energy metabolism during reperfusion of the ischemic working heart.
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