초록 ▼

An atmospheric pressure ion source, e.g. for a mass spectrometer, that produces ions by atmospheric pressure photoionization (APPI). It includes a vaporizer, a photon source for photoionizing vapor molecules upon exit from the vaporizer, a passageway for transporting ions to, for example, a mass spe

An atmospheric pressure ion source, e.g. for a mass spectrometer, that produces ions by atmospheric pressure photoionization (APPI). It includes a vaporizer, a photon source for photoionizing vapor molecules upon exit from the vaporizer, a passageway for transporting ions to, for example, a mass spectrometer system, and a means for directing the ions into the passageway. The center axis of the vaporizer and the center axis of the passageway form an angle that may be about 90 degrees. Included in the invention is a method for creating ions by atmospheric pressure photoionization along an axis and directing them into a passageway oriented at an angle to that axis.

대표청구항 ▼

An atmospheric pressure ion source, e.g. for a mass spectrometer, that produces ions by atmospheric pressure photoionization (APPI). It includes a vaporizer, a photon source for photoionizing vapor molecules upon exit from the vaporizer, a passageway for transporting ions to, for example, a mass spe

An atmospheric pressure ion source, e.g. for a mass spectrometer, that produces ions by atmospheric pressure photoionization (APPI). It includes a vaporizer, a photon source for photoionizing vapor molecules upon exit from the vaporizer, a passageway for transporting ions to, for example, a mass spectrometer system, and a means for directing the ions into the passageway. The center axis of the vaporizer and the center axis of the passageway form an angle that may be about 90 degrees. Included in the invention is a method for creating ions by atmospheric pressure photoionization along an axis and directing them into a passageway oriented at an angle to that axis. EQ ID NO:1, (ii) the amino acid sequence of SEQ ID NO:2, and (iii) the amino acid sequence of SEQ ID NO:3. 8. A progeny plant of the plant of claim 3, wherein the progeny plant comprises the foreign nucleotide sequence. 9. A method for producing a transformed plant having an enhanced ability to resist pathogens compared to a non-transformed plant, comprising: providing a vector comprising a nucleotide sequence encoding a protein, and regulatory elements flanking and operably linked to the nucleotide sequence, the regulatory elements controlling expression of the protein in the plant; and transforming a target plant with the vector to provide a transformed plant, wherein the transformed plant expresses the protein, and wherein the protein has at least about 50% identity to the protein of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and has pathogen resistance activity. 10. The method according to claim 9, wherein the target plant is selected from the group consisting of a monocot and a dicot. 11. The method according to claim 9, wherein the protein comprises a motif having the amino acid sequence of: SEQ ID NO:7 --X-- SEQ ID NO:8 --X-- SEQ ID NO:9 --X-- SEQ ID NO:10 --X-- SEQ ID NO:11, wherein "--X--" represents from zero to about 15 naturally occurring amino acids. 12. The method according to claim 9, wherein the regulatory elements comprise a plant promoter. 13. The method in accordance with claim 9 wherein the protein comprises an amino acid sequence selected from the group consisting of: (I) the amino acid sequence of SEQ ID NO:1, (ii) the amino acid sequence of SEQ ID NO:2, and (iii) the amino acid sequence of SEQ ID NO:3. 14. The method in accordance with claim 9 wherein the nucleotide sequence comprises a sequence selected from the group consisting of: (i) bases 35 to 736 of SEQ. ID NO:4, (ii) bases 2 to 484 of SEQ ID NO:5, and (iii) bases 312 to 1055 of SEQ ID NO:6. 15. A transformed plant obtained according to the method of claim 9. 16. A progeny plant of the plant of claim 15, wherein the progeny plant comprises the nucleotide sequence. 17. A method for transforming a target plant cell, comprising: providing a vector comprising a nucleotide sequence encoding a protein, and regulatory elements flanking and operably linked to the nucleotide sequence, the regulatory elements controlling expression of the protein in a plant; and transforming the target plant cell with the vector to provide a transformed plant cell, wherein the transformed plant cell expresses the protein; and wherein the protein has at least about 50% identity to the protein of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and has pathogen resistance activity. 18. The method according to claim 17, wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:4, SEQ ID NO:5 or SEQ ID NO:6. 19. A transformed plant cell prepared according to the method of claim 17. 20. A method of producing a transformed plant, comprising incorporating into the nuclear genome of the plant an isolated nucleotide sequence which encodes a protein to provide a transformed plant that expresses the protein in an amount effective to enhance the ability of the transformed plant to resist pathogens compared to a nontransformed plant; wherein the protein has at least about 50% identity to the protein of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and has pathogen resistance activity. 21. The method according to claim 20, wherein the protein comprises the amino acid sequence of SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3. 22. The method in accordance with claim 20 wherein the protein comprises a motif having the amino acid sequence of: SEQ ID NO:7 --X-- SEQ ID NO:8 --X-- SEQ ID NO:9 --X-- SEQ ID NO:10 --X-- SEQ ID NO:11, wherein "--X--" represents from zero to about 15 naturally occurring amino acids. 23. The method of claim 20, wherein the protein has at least about 50% identity to the amino acid sequence of SEQ ID NO:1. 24. The method of