[특허]Isoforms of human pregnancy-associated protein-E

Isoforms of human pregnancy-associated protein-E 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-009/50 C12N-015/79 C12N-001/21 C12N-005/10 C12Q-001/37
출원번호	US-0827998 (2001-04-06)
발명자 / 주소	Gu, Yizhong Shannon, Mark E.
출원인 / 주소	Amersham PLC
대리인 / 주소	Fish & Neave
인용정보	피인용 횟수 : 4 인용 특허 : 152

초록 ▼

The invention provides isolated nucleic acids that encode three novel isoforms of human pregnancy associated plasma protein E, hPAPP-E, and fragments thereof, vectors for propagating and expressing PAPP-E nucleic acids, host cells comprising the nucleic acids and vectors of the present invention, proteins, protein fragments, and protein fusions of the novel PAPP-E isoforms, and antibodies thereto. The invention further provides transgenic cells and non-human organisms comprising human PAPP-E isoform nucleic acids, and transgenic cells and non-human organisms with targeted disruption of the endogenous orthologue of the human PAPP-E gene. The invention further provides pharmaceutical formulations of the nucleic acids, proteins, and antibodies of the present invention, and diagnostic, investigational, and therapeutic methods based on the PAPP-E nucleic acids, proteins, and antibodies of the present invention.

대표청구항 ▼

1. An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of: (i) the nucleotide sequence of SEQ ID NO:1; (ii) the nucleotide sequence of SEQ ID NO:2; (iii) a nucleotide sequence that is a degenerate variant of the nucleotide sequence of SEQ ID NO:2; (iv) a sequence of a polynucleotide that encodes a polypeptide with the amino acid sequence of SEQ ID NO:3; and (v) a nucleotide sequence that is the complete complement of the nucleotide sequence of any one of (i)-(iv). 2. The isolated nucleic acid of claim 1, wherein said nucleic acid encodes a polypeptide having metalloproteinase activity. 3. The isolated nucleic acid of claim 1, wherein said nucleic acid is expressed in placental tissue. 4. The isolated nucleic acid of claim 1, further comprising at least one expression control element, wherein said polynucleotide is operably linked to said at least one expression control element. 5. The isolated nucleic acid of claim 1, wherein said isolated nucleic acid is detectably labeled. 6. The isolated nucleic acid of claim 1, wherein said isolated nucleic acid is attached to a substrate. 7. A microarray wherein at least one probe of said microarray is a nucleic acid according to claim 1. 8. A replicable vector comprising the isolated nucleic acid of claim 1. 9. A replicable vector comprising the isolated nucleic acid of claim 4. 10. An expression vector comprising the isolated nucleic acid of claim 1. 11. A host cell transformed to contain the nucleic acid of claim 1, or the progeny thereof. 12. A host cell transformed to contain the replicable vector of claim 8, or the progeny thereof. 13. A host cell transformed to contain the replicable vector of claim 9, or the progeny thereof. 14. A host cell transformed to contain the expression vector of claim 10, or the progeny thereof.

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Isoforms of human pregnancy-associated protein-E 원문보기

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Isoforms of human pregnancy-associated protein-E 원문보기

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