초록 ▼

The present invention pertains to the production of collagen-based products in the form of dispersions and macroporous structures using untreated raw fibrillar type I corium as the starting material. The resulting dispersions have improved characteristics making them ideal for use in environmental a

The present invention pertains to the production of collagen-based products in the form of dispersions and macroporous structures using untreated raw fibrillar type I corium as the starting material. The resulting dispersions have improved characteristics making them ideal for use in environmental applications as a settling aid, a filtration aid, a fractionation medium, an oil droplet stabilizer, a water purification aid, and a water siphoning aid. The dispersions may be further treated in accordance with the methods described herein to form macroporous structures suitable for biotechnological applications including use as a cell culturing substrate and non-biotechnological applications including use as an organic aerogel.

대표청구항 ▼

1. A method of preparing a collagen-based dispersion, comprising the steps of: a) preparing a solution of raw corium, said corium being comprised in part of a plurality of raveled collagen fibers; b) milling said solution of raw corium until said plurality of collagen fibers are substantially un

1. A method of preparing a collagen-based dispersion, comprising the steps of: a) preparing a solution of raw corium, said corium being comprised in part of a plurality of raveled collagen fibers; b) milling said solution of raw corium until said plurality of collagen fibers are substantially unraveled; c) washing and straining the milled raw corium solution of step b); d) centrifuging at 0-5° C. the washed and strained raw corium solution of step c) thereby forming a lower collagen containing phase and an upper aqueous phase; e) repeating steps c) and d), if necessary, until no fats or other material are visibly present in said upper aqueous phase; and f) admixing said lower collagen containing phase with an acid liquid medium thereby forming a collagen dispersion. 2. The method of preparing the collagen-based dispersion of claim 1, wherein said acid is selected from the group consisting of acetic acid, citric acid and oxalic acid. 3. The method of preparing the collagen-based dispersion of claim 1, wherein said collagen dispersion is comprised of 0.25-2.00% collagen, 5.00% acid and 93.00-94.75% deionized water. 4. The method of preparing the collagen-based dispersion of claim 1, wherein said collagen dispersion is milling for approximately 3-5 hours. 5. The method of preparing the collagen-based dispersion of claim 1, wherein additives selected from the group consisting of inert weighting material, pharmaceuticals and biologically active agents are added during said milling of said 1-3% collagen solution. 6. A method of preparing a collagen-based macroporous structure, said macroporous structure comprising an insoluble crosslinked collagen matrix having an open to the surface pore structure; said pores occupying from about 70 to about 99.9% by volume of said macroporous structure wherein said macroporous structure is prepared by the steps of: a) preparing a solution of raw corium, said corium being comprised in part of a plurality of raveled collagen fibers; b) milling said solution of raw corium until said plurality of collagen fibers are substantially unraveled; c) washing and straining the milled raw corium solution of step b); d) centrifuging at 0-5° C. the washed and strained raw corium solution of step c) thereby forming a lower collagen containing phase and an upper aqueous phase; e) repeating steps c) and d), if necessary, until no fats or other material are visibly present in said upper aqueous phase; f) admixing said lower collagen containing phase with an acid liquid medium thereby forming a collagen dispersion; g) rapidly solidifying at least a portion of said collagen dispersion by freezing to form a frozen, solid collagen fiber matrix; h) vacuum drying said collagen fiber matrix into a dry porous matrix; and i) crosslinking the collagen in said dry porous matrix to produce said collagen-based macroporous structure. 7. The method of preparing the collagen-based dispersion of claim 6, wherein said acid is selected from the group consisting of acetic acid, citric acid and oxalic acid. 8. The method of preparing the collagen-based dispersion of claim 6, wherein said collagen dispersion is comprised of 0.25-2.00% collagen, 5.00% acid and 93.00-94.75% deionized water. 9. The method of preparing the collagen-based dispersion of claim 6, wherein said collagen dispersion is milled for approximately 3-5 hours. 10. The method of preparing the collagen-based dispersion of claim 6, wherein additives selected from the group consisting of inert weighting material, pharmaceuticals and biologically active agents are added during said milling of said 1-3% collagen solution. 11. The method of claim 6, further comprising the step of thermally soaking said solid collagen fiber matrix by holding said matrix at a temperature at which said matrix remains frozen and for a period of time and under conditions sufficient to allow partial agglomeration of said collagen fibers to occur.