초록 ▼

A new Musical CD Creation Unit for providing both review and recording functions for the automated merchandising of music entertainment on recordable CDs. The inventive device includes a storage means, a selection means, and a production means. A CD jukebox of the kind commonly available and used in

A new Musical CD Creation Unit for providing both review and recording functions for the automated merchandising of music entertainment on recordable CDs. The inventive device includes a storage means, a selection means, and a production means. A CD jukebox of the kind commonly available and used in commercial markets provides is used to store musical selections. A computer terminal having a display monitor and input means, such as a computer keyboard or a touch screen allows a customer to review and create a musical selection to be reproduced on a recordable CD. Predetermined musical selections allow a customer to make selections based on music type, such as jazz, pop, rock, country, soul, gospel, or blues, popularity, such as "Top 20 Hits," artist, or other options. To provide fully automated merchandising capabilities, the Musical CD Creation Unit has "vending" features including a purchase amount display, a magnetic card reader for credit cards, and a currency changer for cash such that a customer can purchase the recordable CD without assistance from store personnel.

대표청구항 ▼

A new Musical CD Creation Unit for providing both review and recording functions for the automated merchandising of music entertainment on recordable CDs. The inventive device includes a storage means, a selection means, and a production means. A CD jukebox of the kind commonly available and used in

A new Musical CD Creation Unit for providing both review and recording functions for the automated merchandising of music entertainment on recordable CDs. The inventive device includes a storage means, a selection means, and a production means. A CD jukebox of the kind commonly available and used in commercial markets provides is used to store musical selections. A computer terminal having a display monitor and input means, such as a computer keyboard or a touch screen allows a customer to review and create a musical selection to be reproduced on a recordable CD. Predetermined musical selections allow a customer to make selections based on music type, such as jazz, pop, rock, country, soul, gospel, or blues, popularity, such as "Top 20 Hits," artist, or other options. To provide fully automated merchandising capabilities, the Musical CD Creation Unit has "vending" features including a purchase amount display, a magnetic card reader for credit cards, and a currency changer for cash such that a customer can purchase the recordable CD without assistance from store personnel. of antiretroviral therapy of an HIV-infected patient comprising: (i) collecting PBMC from an HIV-infected patient; and (ii) determining whether the PBMC comprise proviral HIV DNA which comprises a mutation at codons 74 or codon 215 , or codons 215 and 74, of the HIV reverse transcriptase gene, in which the presence of the mutations mutation correlates positively with an accelerated immunologic decline of said patient compared with patients who do not have the mutations mutation . 14. The method of claim 13 in which the mutations mutation at codons 74 or codon 215 , or 215 and 74 are is determined by a method comprising polymerase chain reaction. 15. The method of claim 14 which comprises a nested polymerase chain reaction. 16. A method of evaluating the effectiveness of antiretroviral therapy of an HIV-infected patient comprising: (i) collecting a plasma sample from an HIV-infected patient; and (ii) determining whether the plasma sample comprises nucleic acid encoding HIV reverse transcriptase having a mutation at codon 74, in which the presence of the mutation correlates positively with an accelerated immunologic decline of said patient compared to patients who do not have the mutation. 17. The method of claim 16 in which the mutation at codon 74, is determined by a method comprising polymerase chain reaction (PCR). 18. The method of claim 17 which comprises a "nested" polymerase chain reaction. 19. The method of claim 17 which utilizes primer B (5'-GGATGGAAAGGATCACC-3')(SEQ ID NO:9). 20. The method of claim 17 which utilizes primer 3M (3'-AAGTGTGGTCTGTTTTTTGTA-5')(SEQ ID NO:11). 21. The method of claim 17 which utilizes primer 74WT (5'-AAGTTCTCTGAAATCTACTTA-3')(SEQ ID NO:5). 22. The method of claim 17 which utilizes primer 74MUT (5'-AAGTTCTCTGAAATCTACTTC-3')(SEQ ID NO:6). 23. The method of claim 17 which utilizes primer X2(5'-AACAATGGCCATTGACAGA-3')(SEQ ID NO:4). 24. The method of claim 16 in which the antiretroviral therapy being evaluated comprises zidovudine. 25. The method of claim 16 in which the antiretroviral therapy being evaluated comprises didanosine. 26. The method of claim 17 wherein PCR is repeated for 30 to 40 cycles. 27. The method of claim 26 wherein PCR is repeated for 30-35 cycles. 28. A method of evaluating the effectiveness of antiretroviral therapy of an HIV-infected patient comprising: (i) collecting a plasma sample from an HIV-infected patient; and (ii) determining whether the plasma sample comprises nucleic acid encoding HIV reverse transcriptase having a mutation at codons 215 and 74, in which the presence of the mutation correlates positively with an accelerated immunologic decline of said patient compared to patients who do not have the mutation. 29. The method of claim 28 in which the mutation at codons 215 and 74 is determined by a method comprising polymerase chain reaction (PCR). 30. The method of claim 29 which comprises a "nested" polymerase chain reaction. 31. The method of claim 29 which utilizes primer B (5'-GGATGGAAAGGATCACC-3')(SEQ ID NO:9). 32. The method of claim 29 which utilizes primer 3M (3'-AAGTGTGGTCTGTTTTTTGTA-5')(SEQ ID NO:11). 33. The method of claim 29 which utilizes primer 74WT (5'-AAGTTCTCTGAAATCTACTTA-3')(SEQ ID NO:5). 34. The method of claim 29 which utilizes primer 74MUT (5'-AAGTTCTCTGAAATCTACTTC-3')(SEQ ID NO:6). 35. The method of claim 29 which utilizes primer X2(5'-AACAATGGCCATTGACAGA-3')(SEQ ID NO:4). 36. The method of claim 28 in which the antiretroviral therapy being evaluated comprises zidovudine. 37. The method of claim 28 in which the antiretroviral therapy being evaluated comprises didanosine. 38. The method of claim 29 wherein PCR is repeated for 30 to 40 cycles. 39. The method of claim 38 wherein PCR is repeated for 30-35 cycles. 40. A method of evaluating the effectiveness of antiretroviral therapy of an HIV-infected patient comprising: (i) collecting PBMC fro