초록

A method has been discovered for reproducing plants by somatic embryogenesis by placing embryogenic tissue or organ onto a semi-permeable membrane that is on a culture medium and incubating to produce a normal somatic embryo.

대표청구항 ▼

1. A method of reproducing plants by somatic embryogenesis, said method comprising placing embryogenic tissue or organ onto a semi-permeable membrane, wherein said membrane is on a culture medium in a container or said membrane is placed on a culture medium in a container after said embryogenic tiss

1. A method of reproducing plants by somatic embryogenesis, said method comprising placing embryogenic tissue or organ onto a semi-permeable membrane, wherein said membrane is on a culture medium in a container or said membrane is placed on a culture medium in a container after said embryogenic tissue is placed on said membrane; and incubating at a time, temperature and photoperiod effective to produce a normal somatic embryo, wherein said plants are members of the genera Abies, Pinus, Picea, Tsuga, Pseudotsuga, Thuja, Juniperus, Larix, Taxus, Sequoia, or Citrus; wherein said culture medium is a gelled medium. 2. The method according to claim 1, wherein said plants are members of the Citrus genus. 3. The method according to claim 1, wherein said plants are members of Citrus sinensis. 4. The method according to claim 1, wherein said plants are members of Citrus sinensis (L.) Osbeck cv. `Hamlin` or `Valencia.` 5. The method according to claim 1, wherein said semi-permeable membrane is composed of regenerated cellulose, cellulose acetate, cellulose esters, cellulose nitrate, polysulfone, polycarbonate, polyethylene, polyolefin, polypropylene, polyvinylidene fluoride, or mixtures thereof. 6. The method according to claim 1, wherein said semi-permeable membrane is composed of regenerated cellulose, cellulose acetate, cellulose esters, cellulose nitrate, or mixtures thereof. 7. The method according to claim 1, wherein said semi-permeable membrane is composed of cellulose acetate. 8. The method according to claim 1, further comprising transferring said normal somatic embryo to a culture medium and incubating to produce a germinated embryo. 9. The method according to claim 8, further comprising removing said germinated embryo and planting in soil. 10. The method according to claim 1, further comprising incubating said normal somatic embryo to produce a germinated embryo. 11. The method according to claim 10, further comprising removing said germinated embryo and planting in soil. 12. The method according to claim 1, further comprising sealing said container after said placing embryogenic tissue onto a semi-permeable membrane. 13. The method according to claim 12, wherein said incubating is for about 6 weeks at about 27° C. with about a 4-hour photoperiod wherein the light is provided by cool-white fluorescent tubes and the light intensity is between about 15 to about 20 μEm-2s-1.