초록 ▼

An array-based technology facilitates rapid correlated gene copy number and expression profiling of very large numbers of human tumors. Hundreds of cylindrical tissue biopsies (diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block

An array-based technology facilitates rapid correlated gene copy number and expression profiling of very large numbers of human tumors. Hundreds of cylindrical tissue biopsies (diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block. Consecutive sections from such arrays provide targets for parallel in situ visualization and quantitation of DNA, RNA or protein targets. For example, amplifications of six loci (mybL2, erbB2, Cyclin-D1, myc, 17q23 and 20q13) were rapidly determined by fluorescence in situ hybridization from 372 ethanol-fixed breast cancers. Stratification of tumors by estrogen receptor and p53 expression data revealed distinct patterns of gene amplification in the various subgroups of breast cancer that may have prognostic utility. The tissue array technology is useful in the rapid molecular profiling of hundreds of normal and pathological tissue specimens or cultured cells.

대표청구항 ▼

An array-based technology facilitates rapid correlated gene copy number and expression profiling of very large numbers of human tumors. Hundreds of cylindrical tissue biopsies (diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block

An array-based technology facilitates rapid correlated gene copy number and expression profiling of very large numbers of human tumors. Hundreds of cylindrical tissue biopsies (diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block. Consecutive sections from such arrays provide targets for parallel in situ visualization and quantitation of DNA, RNA or protein targets. For example, amplifications of six loci (mybL2, erbB2, Cyclin-D1, myc, 17q23 and 20q13) were rapidly determined by fluorescence in situ hybridization from 372 ethanol-fixed breast cancers. Stratification of tumors by estrogen receptor and p53 expression data revealed distinct patterns of gene amplification in the various subgroups of breast cancer that may have prognostic utility. The tissue array technology is useful in the rapid molecular profiling of hundreds of normal and pathological tissue specimens or cultured cells. d in the form of pulses or periodically for decomposition of said bulk material, and by means for recirculating into the reactor at least a part of a discharged material as circulation material for inoculating the mixture of substances and for accelerating the biological decomposition, the discharged material is moved towards a discharge opening by a discharge means arranged at a bottom portion of the reactor, and when the discharge means moves away from the discharge opening, the discharge means introduces forges into the bulk material. 12. The device according to claim 11, wherein said pressurized air system includes nozzles opening in the bottom portion of said reactor and connected to a pressure accumulator fed by a compressor. 13. The device according to claim 12, wherein air conveying means are arranged for sucking the process air through the mixture of substance. 14. The device according to claim 11, wherein said pressurized air system is associated with a control means through which the pressure of pressurized air or process air may be varied. 15. The device according to claim 11, wherein said pressurized air and process air may be supplied jointly through said pressurized air system. 16. The device according to claim 11, further comprising a gas purification system for purification and recirculation of the process air and/or pressurized air. 17. The device according to claim 11, wherein excitation means is at least partly constituted by the discharge means at the bottom portion of said reactor. 18. The device according to claim 11, wherein a plurality of reactors ate arranged in series, with several reactors arranged in parallel having associated a commnon charging means whereby said mixture of substances to be treated may be supplied. 19. The device according to claim 11, further comprising a compacting means for compacting, dewatering and shaping said mixture of substances which have been treated. 20. The device according to claim 11, wherein at least one dryer is provided for drying the discharged material discharged from the reactor, whereby the at least one dryer includes means for recirculating into the dryer at least a part of the dry material as circulation material. (1988). Kandeler, et al., "Microbioal Biomass, N Mineralization, and the Activities of Various Enzymes in Relation to Nitrate Leaching and Root Distribution in a Slurry-amended Grassland," Biol. Fertil. Soils 18:7-12 (1994). Rowell, et al., "Characteristics Associated with Differences Between Undisturbed and Industrially-Disturbed Soils," Soil Biol. Biochem. 25(11):1499-1511 (1993). Oliver, et al., "The Arginine Ammonification: Determination of Microbial Activity of the Nitrogen Status of Soils?".