IPC분류정보
국가/구분 |
United States(US) Patent
등록
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국제특허분류(IPC7판) |
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출원번호 |
US-0200682
(2002-07-22)
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발명자
/ 주소 |
- Yamazaki, Miki (Vikky)
- Ulman, Morrison
- Nguyen, Luat T.
- Boxer, Steven G.
- Groves, John T.
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 |
피인용 횟수 :
51 인용 특허 :
16 |
초록
▼
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on t
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by a film of aqueous, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Multiplexed assays using the surface detector array device of the present invention are disclosed, as are automated methods for making the surface detector array device that enable formation of arrays wherein the composition of the individual, addressable bilayer regions is unrestricted.
대표청구항
▼
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on t
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by a film of aqueous, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Multiplexed assays using the surface detector array device of the present invention are disclosed, as are automated methods for making the surface detector array device that enable formation of arrays wherein the composition of the individual, addressable bilayer regions is unrestricted. sporiella thermophila, Chaetomium rectopilium, Corynascus thermophilus, Humicola sp., Mycelia sterilia, Myrococcum thermophilum, Myceliophthora thermophila, Rhizomucor miehei, Sporotrichum cellulophilum, Sporotrichum thermophile, Scytalidium indonesicum and Talaromyces thermophilus. 11. The vector of claim 10 wherein the fungus is selected from the group consisting of Acrophialophora levis, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus terreus, Calcarisporiella thermophila, Chaetomium rectopilium, Corynascus thermophilus, Sporotrichum cellulophilum, Sporotrichum thermophile, Mycelia sterilia, Myceliophthora thermophila and Talaromyces thermophilus. 12. The vector of claim 11 wherein the fungus is selected from the group consisting of Aspergillus terreus, Myceliophthora thermophila, Aspergillus fumigatus, Aspergillus nidulans, and Talaromyces thermophilus. 13. The vector of claim 12, wherein the fungus is Aspergillus fumigatus. 14. A vector comprising a polynucleotide derived from Aspergillus fumigatus, which encodes a polypeptide having phytase activity selected from the following: (a) SEQ ID NO: 32; (b) a polynucleotide that hybridizes to the complete complementary strand of SEQ ID NO: 32 under the following conditions: hybridization at 42° C. for 18 hours in a hybridization solution containing 4×SSPE, 50% formamide, 1% SDS, 10% dextran sulfate, 0.5% blotto, and 0.5 mg/ml salmon sperm DNA, wash for 30 minutes at room temperature in 0.1×SSPE containing 0.1% SDS; and (c) a polynucleotide which, because of the degeneracy of the genetic code, does not hybridize with the complete complementary strand of the polynucleotides of (a) or (b), under the following conditions: hybridization at 42° C. for 18 hours in a hybridization solution containing 4×SSPE, 50% formamide, 1% SDS, 10% dextran sulfate, 0.5% blotto, and 0.5 mg/ml salmon sperm DNA, wash for 30 minutes at room temperature in 0.1×SSPE containing 0.1% SDS but which codes for polypeptides having exactly the same amino acid sequences as the polypeptides encoded by the polynucleotides of (a) or (b). 15. A vector comprising a polynucleotide which encodes a polypeptide having phytase activity wherein the poly
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