초록 ▼

A fluid matrix comprising cross-linked remodelable collagen from a donor vertebrate animal is useful for regenerating hydrodynamic function in damaged intervertebral discs in vivo. The matrix may be injectable and may comprise cells and a plurality of purified cell growth factors. The matrix promote

A fluid matrix comprising cross-linked remodelable collagen from a donor vertebrate animal is useful for regenerating hydrodynamic function in damaged intervertebral discs in vivo. The matrix may be injectable and may comprise cells and a plurality of purified cell growth factors. The matrix promotes cell growth and elaboration of proteoglycans to facilitate regeneration of native tissues. The collagen in the matrix may be cross-linked using photooxidative catalysis and visible light, and purified cell growth factors are preferably at least partly bone-derived.

대표청구항 ▼

1. A fluid matrix for treating intervertebral disc disease in a vertebrate, said fluid comprising cross-linked, decellularized nucleus pulposus tissue of a donor vertebrate. 2. The fluid matrix of claim 1 further comprising a growth factor.3. The fluid matrix of claim 2 further comprising a pluralit

1. A fluid matrix for treating intervertebral disc disease in a vertebrate, said fluid comprising cross-linked, decellularized nucleus pulposus tissue of a donor vertebrate. 2. The fluid matrix of claim 1 further comprising a growth factor.3. The fluid matrix of claim 2 further comprising a plurality of living cells.4. The fluid matrix of claim 3, wherein said plurality of living cells comprise chondrocytes other than the chondrocytes of the donor vertebrate.5. The fluid matrix of claim 3, wherein said plurality of living cells comprise mesenchymal stem cells.6. The fluid matrix of claim 3, wherein said plurality of living cells are human-derived.7. The fluid matrix of claim 1, wherein said nucleus pulposus tissue is cross-linked with a photooxidative catalyst.8. The fluid matrix of claim 7, wherein said catalyst is selected from the group consisting of: methylene blue, methylene green, rose bengal, riboflavin, proflavin, fluorescein, eosin, and pyridoxal-5-phosphate.9. The fluid matrix of claim 1, wherein the donor vertebrate is a pig.10. The fluid matrix of claim 1, wherein the donor vertebrate is a cow.11. The fluid matrix of claim 1, wherein the donor vertebrate is a human.