A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particu
A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10−2M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10−2M is achieved.
대표청구항▼
A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particu
A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10−2M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10−2M is achieved. rticipate directly in D1 binding.6. The method of claim 5 wherein the residue which participates directly in D1 binding is located in a region of knob selected from the group consisting of the AB loop, the CD loop, the DE loop, the FG loop, the E strand and the F strand.7. The method of claim 4 wherein the mutation is introduced in a codon encoding the residue of knob which participates directly in D1 binding.8. The method of claim 7 wherein the residue of knob which directly participates in D1 binding is in the AB loop.9. The method of claim 8 wherein the mutation is introduced at the codon for the residue which corresponds to the Ad12 residue selected from the group consisting of 409, 415, 417, 418, 419, 426, and 429.10. The method of claim 9 wherein the residue which directly participates in D1 binding is in the CD loop.11. The method of claim 10 wherein the mutation is introduced at the codon for the residue which corresponds to the Ad12 residue selected from the group consisting of 450 and 451.12. The method of claim 9 wherein the residue which directly participates in D1 binding is in the FG loop of knob.13. The method of claim 12 wherein the mutation is introduced at the codon for the residue which corresponds to the Ad12 residue selected from the group consisting of 517, 519, 520 and 523.14. The method of claim 7 wherein the residue which directly participates in D1 binding is in the E strand of knob.15. The method of claim 14 wherein the mutation is introduced at the codon which encodes the residue corresponding to residue 494 of Adenovirus serotype 12.16. The method of claim 7 wherein the residue which directly participates in D1 binding is in the F strand of knob.17. The method of claim 16 wherein the mutation is introduced at the codon which encodes a residue corresponding to residues 497 and 498 of Adenovirus serotype 12.18. The method of claim 7 wherein the residue which directly participates in D1 binding is in the DE loop of knob.19. The method of claim 18 wherein the mutation is introduced at the codon encoding the residue corresponding to residue 487 of Adenovirus serotype 12.20. The method of claim 2 wherein the introduced mutation results in an amino acid substitution, an amino acid deletion, an amino acid insertion or combination thereof, in the fiber protein knob domain of the encoded viral particle.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (10)
Sasaki Ichiro (Suita JPX) Teraoka Takao (Akashi JPX) Oshima Junji (Toyonaka JPX), Core-shell polymer and its use.
Kawaguchi Haruma (Yokohama JPX) Asai Akira (Fujisawa JPX) Ohtsuka Yasuji (Tokyo JPX) Handa Hiroshi (Tokyo JPX), DNA-immobilized microspheres and a process for purifying a DNA-transcription-controlling factor using the same.
Kausch Albert P. (Stonington CT) Narayanswami Sandya (Irvine CA) Manning Jerry E. (San Clemente CA) Hamkalo Barbara A. (Laguna Beach CA), Isolation of biological materials using magnetic particles.
Hoffman Allan S. (Seattle WA) Monji Nobuo (Seattle WA), Methods for selectively reacting ligands immobilized within a temperature-sensitive polymer gel.
Hooper Herbert H. (Belmont CA) Pacetti Stephen (Sunnyvale CA) Soane David S. (Piedmont CA) Bae Young C. (Seoul KRX), Separation media for electrophoresis.
Hwang, Kyu-youn; Yoo, Chang-eun, Method of isolating a nucleic acid using a material containing an amino group and a carboxyl group and positively charged at a first pH and a solid material for nucleic acid isolation used for the method.
Hwang,Kyu youn; Yoo,Chang eun, Method of isolating a nucleic acid using a material containing an amino group and a carboxyl group and positively charged at a first pH and a solid material for nucleic acid isolation used for the method.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.