초록 ▼

In various embodiments, the invention provides for the use of an ADS1 or ADS2 &Dgr;9 fatty acid desaturase to selectively increase the relative proportion of oleic acid in the fatty acid of a plant part, such as in the oil of a mature seed. In some embodiments, the proportion of oleic acid may be in

In various embodiments, the invention provides for the use of an ADS1 or ADS2 &Dgr;9 fatty acid desaturase to selectively increase the relative proportion of oleic acid in the fatty acid of a plant part, such as in the oil of a mature seed. In some embodiments, the proportion of oleic acid may be increased preferentially, without a corresponding or proportional increase in palmitoleic acid.

대표청구항 ▼

In various embodiments, the invention provides for the use of an ADS1 or ADS2 &Dgr;9 fatty acid desaturase to selectively increase the relative proportion of oleic acid in the fatty acid of a plant part, such as in the oil of a mature seed. In some embodiments, the proportion of oleic acid may be in

In various embodiments, the invention provides for the use of an ADS1 or ADS2 &Dgr;9 fatty acid desaturase to selectively increase the relative proportion of oleic acid in the fatty acid of a plant part, such as in the oil of a mature seed. In some embodiments, the proportion of oleic acid may be increased preferentially, without a corresponding or proportional increase in palmitoleic acid. he ratio of palmitoleic acid to palmitic acid is about 0.1 or less in the part of the genetically modified dicot plant.3. The method of claim 2, wherein the part of the genetically modified dicot plant is a seed.4. The method of claim 3, wherein the DNA sequence is introduced into the dicot plant cell by electroporation, microparticle bombardment, microinjection, or Agrobacterium-mediated transformation.5. The method of claim 1, wherein the DNA sequence encodes Arabidopsis ADS1.6. The method of claim 1, wherein the dicot plant is a Brassica species.7. The method of claim 6, wherein the plant part is an oil extracted from a mature seed of the genetically modified dicot plant.8. A genetically modified dicot plant comprising a heterologous DNA sequence encoding an ADS1 &Dgr;9 fatty acid desaturase, wherein the DNA sequence encodes an ADS1 &Dgr;9 fatty acid desaturase having at least 95% sequence identity to an Arabidopsis ADS1 &Dgr;9 fatty acid desaturase, or wherein the DNA sequence has at least 95% sequence identity to the sequence of SEQ ID NO: 1; and wherein the DNA sequence encoding the ADS1 &Dgr;9 fatty acid desaturase is expressed so that the ratio of oleic acid (18:1) to stearic acid (18:0) is increased by at least 20% in a part of the genetically modified dicot plant, compared to a corresponding part of a non-modified dicot plant, and so that the ratio of palmitoleic acid (16:1) to palmitic acid (16:0) is decreased or remains unchanged or is increased by no more than 20% in the part of the genetically modified dicot plant, compared to the corresponding part of the non-modified dicot plant. 9. The genetically modified dicot plant as claimed in claim 8, wherein the dicot plant is a Brassica species and the ratio of oleic acid to stearic acid in the part of the genetically modified plant is about 35 or greater and the ratio of palmitoleic acid to palmitic acid is about 0.1 or less in the part of the genetically modified dicot plant.10. The genetically modified dicot plant as claimed in claim 9, wherein the part of the genetically modified dicot plant is a seed.11. The genetically modified dicot plant as claimed in claim 8, wherein the DNA sequence encodes Arabidopsis ADS1.12. The genetically modified dicot plant as claimed in claim 8, wherein the dicot plant is a Brassica species.13. The genetically modified dicot plant of claim 12, wherein the plant part is an oil extracted from a mature seed of the genetically modified dicot plant.14. The method of claim 1, wherein the DNA sequence has the sequence of SEQ ID NO: 1.15. The genetically modified dicot plant of claim 8, wherein the DNA sequence has the sequence of SEQ ID NO: 1.16. The method of claim 6, wherein the Brassica species isBrassica napus.17. The method of claim 6, wherein the Brassica species isBrassica rapa.18. The method of claim 6, wherein the Brassica species isBrassica juncea.19. The genetically modified dicot plant of claim 12, wherein the Brassica species isBrassica napus.20. The genetically modified dicot plant of claim 12, wherein the Brassica species isBrassica rapa.21. The genetically modified dicot plant of claim 12, wherein the Brassica species isBrassica juncea.22. A method for modifying a Brassica junceaplant, comprising the steps of: a) introducing a DNA sequence encoding an Arabidopsis ADS1 &Dgr;9 fatty acid desaturase, or having the sequence of SEQ ID NO: 1, into a plant cell of the Brassica junceaplant, or an ancestor of theBrassica junceaplant, to produce a genetically modifiedBrassica junceaplant comprising the DNA sequence; b) maintaining the genetically modified Brassica junceaplant under conditions so that the DNA sequence encoding the Arabidopsis ADS1 &Dgr;9 fatty acid desaturase, or having the sequence of SEQ ID NO: 1, is expressed; and so that the ratio of oleic acid (18:1) to stearic acid (18:0) is increased by at least 20% in a part of the genetically modifiedBrassica junceaplant, compared to a corresponding part of a non-modifie