Integrated active flux microfluidic devices and methods
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C12P-019/34
C12M-001/34
C07H-021/02
C07H-021/04
출원번호
US-0875438
(2001-06-05)
발명자
/ 주소
Quake, Stephen R.
Chou, Hou-Pu
출원인 / 주소
California Institute of Technology
대리인 / 주소
Darby & Darby
인용정보
피인용 횟수 :
314인용 특허 :
19
초록▼
The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecul
The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
대표청구항▼
1. A method for mixing two or more different fluids in a microfluidic device, which method comprises:(a) introducing the different fluids to a microfluidic device, the microfluidic device having:(i) a circulation loop channel communicating with at least one service channel,(ii) a microvalve separati
1. A method for mixing two or more different fluids in a microfluidic device, which method comprises:(a) introducing the different fluids to a microfluidic device, the microfluidic device having:(i) a circulation loop channel communicating with at least one service channel,(ii) a microvalve separating the circulation loop channel from the service channel, and(iii) a peristaltic pump formed within the microfluidic device and associated with the circulation loop channel,so that each of the different fluids is loaded into the circulation loop channel; and(b) activating the peristaltic pump associated with the circulation loop channel to circulate at least one of the different fluids about the circulation loop channel to mix the different fluids. 2. A method according to claim 1, wherein the microfluidic device has at least one inlet channel and at least one outlet channel, the different fluids being loaded into the circulation loop channel through at least one inlet channel. 3. A method according to claim 2, wherein:the microfluidic device has two or more inlet channels; andeach of the two or more different fluids is loaded into the circulation loop channel through a different inlet channel. 4. A method according to claim 2, wherein each inlet channel and each outlet channel is separated from the circulation loop channel by a microvalve. 5. A method according to claim 4, wherein the micro valves separating the inlet and outlet channels from the circulation loop channel are closed while the peristaltic pump is activated. 6. A method according to claim 4, wherein the micro valves separating the inlet and outlet channels from the circulation loop channel remain open while the peristaltic pump is activated. 7. A method according to claim 1 wherein the peristaltic pump comprises at least three cooperating micro valves acting within the circulation loop channel. 8. A method according to claim 1, wherein at least one of the different fluids comprises a solution of molecules. 9. A method according to claim 8, wherein the solution of molecules contains molecules selected from a group consisting of: nucleic acid molecules, polypeptide molecules, antibody molecules. 10. A method according to claim 1, wherein at least one of the different fluids comprises a suspension of particles. 11. A method according to claim 10, wherein the suspension of particles contains particles selected from a group consisting of: cells, virions, and microscopic beads. 12. A method for binding a sample to a target, which method comprises:(a) introducing a fluid containing the sample to a microfluidic device, the microfluidic device having:(i) a circulation loop channel communicating with at least one service channel and having molecules of the target disposed therein,(ii) a micro valve separating the circulation loop channel from the service channel, and(iii) a peristaltic pump formed within the microfluidic device and associated with the peristaltic loop channel,so that the fluid containing the sample is loaded into the circulation loop channel; and(b) activating the peristaltic pump so that the fluid circulates through the loop, wherein the sample binds to the target molecules disposed in the loop as the fluid circulates therethrough. 13. A method according to claim 12, wherein the target molecules are selected from a group consisting of polynucleotide probes, protein probes, antibody probes, biotin and avidin. 14. A method according to claim 12, wherein the sample comprises nucleic acid molecules, protein molecules, cells or virions. 15. A method according to claim 14, wherein: the sample comprises nucleic acid molecules, andthe target comprises polynucleotide probes specific for nucleic acid molecules in the sample. 16. A method according to claim 14, wherein: the target comprises biotin molecules, andthe sample comprises particles or molecules having a biotin specific label. 17. A method according to claim 16, wherein the biotin specific label is avidin or Ne utrAvidin. 18. A method according to claim 12, wherein the peristaltic pump comprises at least three cooperating micro valves acting within the circulation loop channel. 19. A method according to claim 12, wherein the circulation loop channel resides in a layer of elastomeric material. 20. A method according to claim 19, wherein the layer of elastomeric material is adjacent to a surface of a substrate layer. 21. A method according to claim 20, wherein the surface of the substrate layer has molecules of the target attached thereto.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (19)
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Array of oligonucleotides on a solid substrate.
Chee Mark ; Cronin Maureen T. ; Fodor Stephen P. A. ; Huang Xiaohua X. ; Hubbell Earl A. ; Lipshutz Robert J. ; Lobban Peter E. ; Morris MacDonald S. ; Sheldon Edward L., Arrays of nucleic acid probes on biological chips.
Wise Kensall D. (Ann Arbor MI) Robertson Janet K. (Ann Arbor MI) Ji Jin (White Plains NY), Integrated microvalve structures with monolithic microflow controller.
Benecke Wolfgang (Berlin DEX) Wagner Bernd (Berlin DEX) Hagedorn Rolf (Berlin DEX) Fuhr Gnter (Berlin DEX) Mller Torsten (Berlin DEX), Method of continuously separating mixtures of microscopic dielectric particles and apparatus for carrying through this m.
Aine Harry E. (30600 Page Mill Rd. Los Altos CA 94022) Block Barry (30610 Page Mill Rd. Los Altos CA 94022), Miniature valve and method of making same.
Hollis Mark A. ; Ehrlich Daniel J. ; Murphy R. Allen ; Kosicki Bernard B. ; Rathman Dennis D. ; Mathews Richard H. ; Burke Barry E. ; Eggers Mitch D. ; Hogan Michael E. ; Varma Rajender Singh, Optical and electrical methods and apparatus for molecule detection.
Chow Calvin Y. H. ; Parce J. Wallace, Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forc.
Chow Calvin Y. H. ; Parce J. Wallace, Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forc.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Mark A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake,Stephen R.; Unger,Marc A.; Chou,Hou Pu; Thorsen,Todd A.; Scherer,Axel, Apparatus and methods for conducting assays and high throughput screening.
Durack, Gary; Wallace, Jeffrey D.; Vandre, Gary P.; Westfall, Lon A.; Hatcher, Jeremy T.; Nayak, Niraj V., Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm.
Ness, Kevin Dean; Burd, Samuel; Hindson, Benjamin Joseph; Belgrader, Phillip; Colston, Jr., Billy W., Cartridge with lysis chamber and droplet generator.
Grossman, Robert; Unger, Marc; Lam, Phillip; Chou, Hou-Pu; Kimball, Jake; Pieprzyk, Martin, Crystal forming devices and systems and methods for using the same.
Bell, H. Randall; Bienvenue, Joan M; Pettit, John W; Landers, James P; Norris, Jessica V; Scott, Orion N; Marchiarullo, Daniel J; Leslie, Daniel C, DNA analyzer.
Colston, Jr., Billy Wayne; Hindson, Benjamin Joseph; Ness, Kevin Dean; Masquelier, Donald Arthur; Milanovich, Fred Paul; Modlin, Douglas N.; Riot, Vincent; Burd, Samuel; Makarewicz, Jr., Anthony Joseph; Belgrader, Phillip, Droplet-based assay system.
Fournier-Bidoz, Sebastian; Chan, Warren Che Wor, Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto.
Fournier-Bidoz, Sebastien; Chan, Warren Che Wor, Flow focusing method and system for forming concentrated volumes of microbeads, and microbeads formed further thereto.
Xiang, Qing; Osborne, Jill Lindsey; Davey, Ryan; Dupoteau, Francois, Handheld diagnostic test device and method for use with an electronic device and a test cartridge in a rapid diagnostic test.
Seidel, George E.; Lu, Kehuan; Suh, Tae Kwang, In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations.
Durack, Gary; Wallace, Jeffrey D.; Vandre, Gary P.; Westfall, Lon A.; Hatcher, Jeremy T.; Nayak, Niraj V., Method and apparatus for calibrating a flow cytometer.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Ceasar, Christopher G.; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Ceasar, Christopher; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Cesar, Christopher G.; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Cesar, Christopher G.; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Beebe, David J.; Meyvantsson, Ivar; Warwick, Jay W.; Toepke, Michael, Method for controlling communication between multiple access ports in a microfluidic device.
Chung,Yung Chiang; Chang,Yao Sung; Shiu,Ming Zheng, Method for nucleic acid hybridization using back and forth flow between a denaturation channel and a hybridization channel via a connection channel.
Unger,Marc A.; Chou,Hou Pu; Thorsen,Todd A.; Scherer,Axel; Quake,Stephen R.; Liu,Jian; Adams,Mark L.; Hansen,Carl L., Method of forming a via in a microfabricated elastomer structure.
Seidel, George E.; Suh, Tae Kwang; Lu, Kehuan, Method of in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations.
Barnhizer, Bret T.; Gazenko, Sergey, Methods and devices for rapid detection and identification of live microorganisms by aptamers and/or antibodies immobilized on permeable membranes.
Putnam, Martin A.; Branciforte, Jeffrey T.; Stanwood, Charles O., Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays.
Putnam, Martin A.; Branciforte, Jeffrey T.; Stanwood, Charles O., Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C; Toppani, Dominique; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods for multiple single-cell capturing and processing using micro fluidics.
Seidel, George E.; Suh, Tae Kwang; Lu, Kehuan; Cran, David G., Methods for separating frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C.; Toppani, Dominique G.; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods, systems and devices for multiple single-cell capturing and processing using microfluidics.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C.; Toppani, Dominique G.; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods, systems and devices for multiple single-cell capturing and processing using microfluidics.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C.; Toppani, Dominique G.; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods, systems and devices for multiple single-cell capturing and processing using microfluidics.
Putnam, Martin A.; Branciforte, Jeffrey T.; Stanwood, Charles O.; Tu, Jane M., Micro-tube particles for microfluidic assays and methods of manufacture.
Begley, Matthew R.; Landers, James P.; Ferrance, Jerome P.; Huang, Ling; Jones, Michael H.; Utz, Marcel; Barker, Scott, Microdevices for chemical sensing and chemical actuation.
Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel; Quake, Stephen R.; Enzelberger, Markus M.; Adams, Mark L.; Hansen, Carl L., Microfabricated elastomeric valve and pump systems.
Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel; Quake, Stephen R.; Enzelberger, Markus M.; Adams, Mark L.; Hansen, Carl L., Microfabricated elastomeric valve and pump systems.
Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel; Quake, Stephen R.; Enzelberger, Markus M.; Adams, Mark L.; Hansen, Carl L., Microfabricated elastomeric valve and pump systems.
Maerkl, Sebastian J.; Thorsen, Todd A.; Bao, Xiaoyan; Quake, Stephen R.; Studer, Vincent, Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors.
Spuhler, Philipp S.; Smith, Kyle C.; Fachin, Fabio; Barber, Thomas Alan; Kapur, Ravi; Toner, Mehmet; Pai, Vincent; Karabacak, Murat N., Microfluidic sorting using high gradient magnetic fields.
Durack, Gary; Wallace, Jeffrey D.; Vandre, Gary P.; Westfall, Lon A.; Hatcher, Jeremy T.; Nayak, Niraj V., Multi-channel system and methods for sorting particles.
Durack, Gary; Wallace, Jeffrey D.; Vandre, Gary P.; Westfall, Lon A.; Hatcher, Jeremy T.; Nayak, Niraj V., Multi-channel system and methods for sorting particles.
Bell, H. Randall; Pettit, John W; Landers, James P; Bienvenue, Joan M; Marchiarullo, Daniel J; Root, Brian E; Scott, Orion N, Optical approach for microfluidic DNA electrophoresis detection.
Putnam, Martin A.; Leamon, John H.; Branciforte, Jeffrey T.; Stanwood, Charles O., PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use.
Foster, John Stuart; Shields, Kevin Eugene; Naivar, Mark; Hoonejani, Mehran Rajaian, Particle manipulation system with out-of-plane channel and recovery algorithm.
DeSimone, Joseph M.; Rolland, Jason P.; Quake, Stephen R.; Schorzman, Derek A.; Yarbrough, Jason; Van Dam, Michael, Photocurable perfluoropolyethers for use as novel materials in microfluidic devices.
Durak, Gary; Wallace, Jeffrey D.; Vandre, Gary P.; Westfall, Lon A.; Hatcher, Jeremy T.; Nayak, Niraj V., Process for evaluating staining conditions of cells for sorting.
Seidel, George E.; Lu, Kehuan; Suh, Tae Kwang; Cran, David G., Separation systems of frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations.
Dupoteau, Francois; Dekel, Shlomit, Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples.
Dupoteau, Francois; Dekel, Shlomit, Single-use handheld diagnostic test device, and an associated system and method for testing biological and environmental test samples.
Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for droplet-based assays using an array of emulsions.
Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for forming an array of emulsions.
Hiddessen, Amy L.; Masquelier, Donald A.; Ness, Kevin D.; Hindson, Benjamin J.; Makarewicz, Jr., Anthony J.; Chia, Erin R., System for forming emulsions.
Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A.; Chia, Erin R., System for generating droplets with pressure monitoring.
Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A.; Chia, Erin R., System for generating droplets with push-back to remove oil.
Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for transporting emulsions from an array to a detector.
Seidel, George E.; Lu, Kehuan; Suh, Tae Kwang; Cran, David G., System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations.
Zenhausern, Frederic; Orozco, Christine; Richards, Mark; Yamashiro, Carl; Amundson, Sally A.; Lenigk, Ralf; Bittner, Michael L.; Balagurunathan, Yoganand, Systems and methods for biodosimetry with biochip using gene expression signatures.
Chan, Warren Che Wor; Jennings, Travis Leon; Klostranec, Jesse M., Systems and methods for enhancing fluorescent detection of target molecules in a test sample.
Zhang, Jian; Salerno, Alessio; Simaan, Nabil; Yao, Y. Lawrence; Randers-Pehrson, Gerhard; Garty, Guy; Dutta, Aparajita; Brenner, David J., Systems and methods for robotic transport.
Unger, Marc; Manger, Ian D.; Lucero, Michael; Yi, Yong; Miyashita-Lin, Emily; Wienecke, Anja; Facer, Geoffrey, Thermal reaction device and method for using the same.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.