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특허 상세정보

Methods for producing poly(hydroxy) fatty acids in bacteria

국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12P-007/64    C12P-021/06    C12P-001/00    C12N-001/20    C07H-021/04   
미국특허분류(USC) 435/134; 435/069.1; 435/320.1; 435/471; 435/252.34; 435/253.3; 435/041; 435/173.8; 536/023.1; 536/023.2; 536/023.7; 500/350
출원번호 US-0779427 (2001-02-08)
우선권정보 DE-44 33 134 (1994-09-16)
발명자 / 주소
출원인 / 주소
대리인 / 주소
    Pabst Patent Group LLP
인용정보 피인용 횟수 : 38  인용 특허 : 0
초록

The present invention relates to a process for the production of poly (hydroxy fatty acids) as well as recombinant bacterial strains for carrying out the process. In addition, new poly(hydroxy fatty acids) and new substrates for the production of conventional and new poly(hydroxy fatty acids) are described. Moreover, the invention also relates to a DNA fragment, which codes for a PhaE and a PhaC component of the poly(hydroxy fatty acid) synthase from Thiocapsa pfennigii , as well as the corresponding poly (hydroxy fatty acid) synthase protein.

대표
청구항

1. A process for the preparation of poly(hydroxy fatty acids) comprising incubating a recombinant bacteria in a mineral medium under aerobic conditions, expressing at least one fragment of the gene encoding the poly(hydroxy fatty acid) synthase from Thiocapsa pfennigii with a substrate carbon source, wherein the recombinant organism produces a poly(hydroxy fatty acid) and, the poly(hydroxy fatty acid) are recovered. 2. The process of claim 1, wherein the bacteria are pre-cultivated in a complex medium. 3. The process of claim 1, wherein one also adds to...

이 특허를 인용한 특허 피인용횟수: 38

  1. Burk, Mark J.; Van Dien, Stephen J.; Burgard, Anthony P.; Niu, Wei. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors. USP2011118067214.
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  3. Burk, Mark J.; Van Dien, Stephen J.; Burgard, Anthony P.; Niu, Wei. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors. USP2013018357520.
  4. Burk, Mark J.; Van Dien, Stephen J.; Burgard, Anthony P.; Niu, Wei. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors. USP2016119487803.
  5. Burk, Mark J.; Van Dien, Stephen J.; Burgard, Anthony P.; Niu, Wei. Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors. USP2015038969054.
  6. Herrema, Markus; Kimmel, Kenton. Energy production systems utilizing ruminant animal methane emissions. USP2015028945915.
  7. Herrema, Markus; Kimmel, Kenton. Method for producing polyhydroxyalkanoic acid. USP2012098263373.
  8. Herrema, Markus; Kimmel, Kenton. Method for producing polyhydroxyalkanoic acid. USP2014048703470.
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  10. Burgard, Anthony P.; Van Dien, Stephen J.; Burk, Mark J.. Methods and organisms for the growth-coupled production of 1,4-butanediol. USP2013068470582.
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  12. Burgard, Anthony P.; Osterhout, Robin E.; Sun, Jun; Pharkya, Priti. Methods for increasing product yields. USP2012088236539.
  13. Burgard, Anthony P.; Osterhout, Robin E.; Sun, Jun; Pharkya, Priti. Methods for increasing product yields. USP2013058445244.
  14. Burgard, Anthony P.; Osterhout, Robin E.; Sun, Jun; Pharkya, Priti. Methods for increasing product yields. USP2014018637286.
  15. Burgard, Anthony P.; Osterhout, Robin E.; Sun, Jun; Pharkya, Priti. Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes. USP2011118048661.
  16. Pharkya, Priti; Burgard, Anthony P.; Van Dien, Stephen J.; Osterhout, Robin E.; Burk, Mark J.; Trawick, John D.; Kuchinskas, Michael P.; Steer, Brian. Microorganisms and methods for production of 4-hydroxybutyrate, 1,4-butanediol and related compounds. USP2017069677045.
  17. Sun, Jun; Burk, Mark J.; Burgard, Anthony P.; Osterhout, Robin E.; Niu, Wei; Trawick, John D.; Haselbeck, Robert. Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone. USP2015129222113.
  18. Sun, Jun; Burk, Mark J.; Burgard, Anthony P.; Osterhout, Robin E.; Niu, Wei; Trawick, John D.; Haselbeck, Robert. Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone. USP2013098530210.
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  25. Van Dien, Stephen J.; Burgard, Anthony P.; Haselbeck, Robert; Pujol-Baxley, Catherine J.; Niu, Wei; Trawick, John D.; Yim, Harry; Burk, Mark J.; Osterhout, Robin E.; Sun, Jun. Microorganisms for the production of 1,4-butanediol and related methods. USP2012038129169.
  26. Haselbeck, Robert; Trawick, John D.; Niu, Wei; Burgard, Anthony P.. Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds, and methods related thereto. USP2013028377667.
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  36. Clark, Warren; Japs, Michael; Burk, Mark J.. Process of separating components of a fermentation broth. USP2013128597918.
  37. Yu,Jian. Production of biodegradable thermoplastic materials from organic wastes. USP2006117141400.
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