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Provided are methods of screening and identification of bio activities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecul

Provided are methods of screening and identification of bio activities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.

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1. A method for identifying an enzymatic activity of interest, comprising:(a) introducing a fluorogenic or chromogenic substrate and a recombinant clone into a capillary tube of a capillary array, wherein each capillary tube of the capillary array comprises at least one wall defining a lumen for ret

1. A method for identifying an enzymatic activity of interest, comprising:(a) introducing a fluorogenic or chromogenic substrate and a recombinant clone into a capillary tube of a capillary array, wherein each capillary tube of the capillary array comprises at least one wall defining a lumen for retaining the substrate and the recombinant clone, and wherein the at least one wall is comprised of a material having a low refractive index;(b) culturing the clone in the capillary tube containing the substrate and the recombinant clone under conditions which allow interaction of the substrate and an enzyme produced by the recombinant clone to produce a detectable optical signal; and(c) detecting the detectable optical signal in the capillary tube to identify one or more capillaries containing the detectable optical signal, thereby identifying the enzymatic activity of interest. 2. The method of claim 1, wherein the recombinant clone is from a library of clones. 3. The method of claim 2, wherein the library is an expression library. 4. The method of claim 3, wherein the expression library is generated from genomic DNA of one or more microorganisms. 5. The method of claim 4, wherein the microorganisms are marine microorganisms. 6. The method of claim 4, wherein the microorganisms are airborne microorganisms. 7. The method of claim 4, wherein the microorganisms comprise prokaryotic cells. 8. The method of claim 4, wherein the microorganisms are derived from an environmental sample. 9. The method of claim 4, wherein the microorganisms are terrestrial microorganisms. 10. The method of claim 4, wherein the microorganisms comprise extremophiles. 11. The method of claim 10, wherein the extremophiles are thermophiles. 12. The method of claim 10, wherein the extremophiles are selected from the group consisting of hyperthernophiles, psychrophiles, halophiles, psychrotrophs, alkalophiles and acidophiles. 13. The method of claim 4, wherein the host cells are selected from the group consisting of bacterial cells, fungal cells, plant cells, insect cells and animal cells. 14. The method of claim 4, wherein the host cells are prokaryotic cells. 15. The method of claim 14, wherein the prokaryotic cells are bacterial cells. 16. The method of claim 15, wherein the bacterial cells are E. coli , Pseudomonas, or Bacillus. 17. The method of claim 1, wherein the enzyme is a hydrolase. 18. The method of claim 1, wherein the detectable optical signal is a change in color. 19. The method of claim 1, wherein the detectable optical signal is a change in fluorescence. 20. The method of claim 19, wherein the substrate is selected from the group consisting of umbelliferone, resorufin, fluorescein rhodamine, and derivatives thereof. 21. The method of claim 1, wherein the detection is provided by a detector comprising a photodetector. 22. The method of claim 1, wherein the capillary array comprises at least about 100 capillary tubes. 23. The method of claim 1, wherein the capillary array comprises at least about 1000 capillary tubes. 24. The method of claim 1, wherein the capillary array comprises at least about 5000 capillary tubes. 25. The method of claim 1, wherein the capillary array comprises at least about 100,000 to about 4,000,000 capillary tubes. 26. The method of claim 1, wherein the capillary array has a capillary tube density of about 500 capillary tubes/cm 2 to 51,000 capillary tubes/cm 2 . 27. The method of claim 1, wherein the height:width aspect ratio of the capillary army is at least about 1. 28. The method of claim 1, wherein the substrate and the recombinant clone are introduced simultaneously as a cell/substrate mixture into capillary tubes in the capillary army. 29. The method of claim 1, further comprising biopanning prior to step (a). 30. The method of claim 4, further comprising normalizing the genomic DNA samples prior to generating the library. 31. The method of claim 1, further comprising the step of isolating one or more enzymes from t he recovered contents of the one or more capillary tubes. 32. The method of claim 1, further comprising recovering and sequencing a polynucleotide from the recombinant clone encoding the desired enzymatic activity. 33. The method of claim 1, wherein the substrate comprises C12FDG. 34. The method of claim 1, further comprising after (c):(d) recovering the contents of the capillary tube containing the detectable signal, thereby recovering the enzymatic activity of interest. 35. The method of claim 1, wherein the enzyme is a lipase. 36. The method of claim 1, wherein the enzyme is a hydratase 37. The method of claim 1, wherein the enzyme is an esterase. 38. The method of claim 1, wherein the enzyme is a protease. 39. The method of claim 1, wherein the enzyme is a peptidase. 40. The method of claim 1, wherein the enzyme is a reductase. 41. The method of claim 1, wherein the enzyme is an oxidoreductase. 42. The method of claim 1, wherein the enzyme is a lyase. 43. The method of claim 1, wherein the enzyme is a ligase. 44. The method of claim 1, wherein the enzyme is an isomerase. 45. The method of claim 1, wherein the enzyme is a polymerase. 46. The method of claim 1, wherein the enzyme is a synthetase. 47. The method of claim 1, wherein the enzyme is a glycosidase. 48. The method of claim 1, wherein the enzyme is a transferase. 49. The method of claim 1, wherein the enzyme is a phosphatase. 50. The method of claim 1, wherein the enzyme is a kinase. 51. The method of claim 1, wherein the enzyme is a mono- or di-oxygenase. 52. The method of claim 1, wherein the enzyme is a nitrilase. 53. The method of claim 1, wherein the enzyme is a peroxidase. 54. The method of claim 1, wherein the enzyme is a transaminase. 55. The method of claim 1, wherein the enzyme is an amidase. 56. The method of claim 1, wherein the enzyme is an acylase.