IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0949314
(2001-09-07)
|
발명자
/ 주소 |
- Mahant, Vijay
- Doneen, Byron
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
10 인용 특허 :
1 |
초록
▼
A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another ste
A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another step, an additive and particles are added to the sample, wherein the additive substantially binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the particles and wherein the particles substantially bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive, thereby producing a cell-containing network. In a further step, the network is separated from the substantially cell-depleted portion by applying a magnetic force.
대표청구항
▼
1. A method of separating a target cell-containing sample into a substantially target cell-depleted portion, and a target cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte, comprising:receiving the sample in a vessel, wherein the vessel retains the sample
1. A method of separating a target cell-containing sample into a substantially target cell-depleted portion, and a target cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte, comprising:receiving the sample in a vessel, wherein the vessel retains the sample within a plurality of confining walls, and wherein at least one of the confining walls is flexible;providing an additive and magnetic particles, wherein the additive binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the magnetic particles, and wherein the magnetic particles bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive;combining the sample, the additive, and the magnetic particles to produce a target cell-containing network; andseparating the network within the plurality of confining walls from the target cell-depleted portion by applying a first and a second force, wherein the first force is a magnetic force and the second force is an automatic mechanical force that is transmitted through at least one of the confining walls. 2. The method of claim 1 wherein the target cell-containing portion comprises a stem cell. 3. The method of claim 2 wherein the stem cell is selected from the group consisting of an embryonic stem cell, an adult stem cell, and a partially differentiated stem cell. 4. The method of claim 3 wherein the stem cell has a surface marker selected from the group consisting of SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81. 5. The method of claim 1 wherein the sample received in the vessel has a volume of between about 10 ml and 100 ml. 6. The method of claim 1 wherein the sample received in the vessel has a volume of between about 100 ml and 500 ml. 7. The method of claim 1 wherein the particles are coated with a coating. 8. The method of claim 7 wherein the coating comprises an anti-ligand. 9. The method of claim 7 wherein the coating comprises an antibody. 10. The method of claim 1 wherein the magnetic particles comprise a primary antibody and the additive comprises a secondary antibody, and wherein the primary antibody binds to a surface component of the at least one of the stem cell, lymphocyte, and leukocyte, and wherein the secondary antibody binds to the primary antibody. 11. The method of claim 1 wherein separating the network produces a separation efficiency of at least 90%. 12. The method of claim 1 wherein at least 90% by volume of the cell depleted portion is separated from the network within ten minutes, with a separation efficiency of at least 95%.
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