[특허]Formulation for reducing urea effect for immunochromatography assays using urine samples

Formulation for reducing urea effect for immunochromatography assays using urine samples 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	G01N-033/53
출원번호	US-0925998 (1997-09-09)
발명자 / 주소	Rheinheimer, Gary W. Yip, Meitak Teresa
출원인 / 주소	Bayer Corporation
인용정보	피인용 횟수 : 5 인용 특허 : 23

초록 ▼

Disclosed is an improvement in the analysis for an analyte in a urine test sample in which the urine is contacted with a labeled antibody specific to the analyte and the concentration of the analyte is determined by measuring the response from the label. The improvement involves maintaining the concentration of urea in the test sample above the threshold value which value is the concentration of urea at which increases in the urea concentration do not affect the accuracy of the assay such as by interfering with the binding between the analyte and the labeled antibody.

대표청구항 ▼

1. In an analysis for an analyte in a urine test sample in which antibodies having a detectable label and which are specific to the analyte are combined with the urine test sample and the concentration of the analyte in the urine test sample is determined by the amount of binding of the antibodies having the detectable label with the analyte by measuring the magnitude of the response from the detectable label and comparing the magnitude of the response from the detectable label with responses obtained from calibrators containing similar amounts of urea, the improvement which comprises determining a threshold of urea concentration in the urine test sample which level is greater than that normally found in urine and at which increases in the urea concentration do not affect the accuracy of the analysis and maintaining the concentration of urea in the urine test sample above the threshold value by adding urea thereto. 2. The analysis of claim 1 which is carried out in a matrix of an absorbant material through which the urine test sample, the analyte and the labeled antibody flow by capillarity. 3. The analysis of claim 2 wherein the matrix of absorbent material is nitrocellulose, a polysulfone, a polycarboxylic acid or filter paper. 4. The analysis of claim 1 wherein the analyte is deoxypyridinoline and sufficient urea is combined with the urine test sample to provide a urea concentration therein of greater than 225 mM. 5. The analysis of claim 1 carried out in a microtiter plate. 6. The analysis of claim 1 wherein the analysis carried out in a matrix of an absorbant material in the form of a strip wherein the labeled antibodies are stored in a distinct portion of the strip and which strip has a buffer containing pad upstream from the labeled antibodies in which the urea necessary to increase the concentration of urea in the urine test sample is stored. 7. A test strip for the determination of an analyte in a urine test sample which test strip comprises a matrix of an absorbent material through which the urine test sample, the analyte and antibodies which bear a detectable label can flow by capillarity and which strip has a region containing mobile antibodies bearing a detectable label and which are specific for the analyte and, upstream from the labeled antibody containing region, a region containing dry urea in sufficient quantity so that when the urea is rehydrated upon contact with the urine test sample when the urine test sample is applied to the strip there will be provided a concentration of urea in the urine test sample which is above the level normally found in urine and which has been determined to be above a threshold value at which increases in the urea concentration do not affect binding between the analyte and the antibodies bearing the detectable label. 8. The test strip of claim 7 in which the region containing the antibodies bearing the detectable label also contains a buffer. 9. The test strip of claim 8 wherein the antibodies are specific to deoxypyridinoline and the concentration of urea in the dry urea containing region of the strip is sufficient to raise the concentration of urea in the urine test sample to greater than 225 mM upon application of the urine test sample to the strip and rehydration of the dry urea by the so applied urine test sample. 10. An assay for deoxypyridinoline in urine which comprises applying a urine test sample to a test strip of nitrocellulose which strip has a region containing gold sol labeled antibodies specific for deoxypyridinoline and upstream from the gold sol labeled antibody containing region there is a region containing dry urea in sufficient concentration to raise the level of urea in the urine test sample when it is rehydrated upon contact with the urine test sample to a level of greater than 225 mM.

이 특허에 인용된 특허 (23)

de Steenwinkel Floris (Brussels BEX) Collet-Cassart Daniel (Wavre BEX) Masson Pierre L. (Brussels BEX), Agglutination immunoassays carried out with agent to reduce non-specific interferences.
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Schick Lloyd A. (Bristol IN) Yip Meitak T. (Elkhart IN), Ascorbate interference-resistant composition, device and method of assaying for predetermined analyte.
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Kuzuya Keiko,JPX ; Yamauchi Tadakazu,JPX, Assay utilizing hydrogen peroxide adduct.
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Jones Ronald M. ; Barr Eric G. ; Hewett Gary E., Automated immunoassay cassette.
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Neurath A. Robert (New York NY), Composition for use in immunoassays.
상세보기
Johnson Gary M., Conversion of pyridinoline to deoxypyridinoline.
상세보기
Daniloff Yuri (Mountain View CA) Robins Simon P. (Aberdeen CA GB6) Evans Brian J. (Santa Clara CA) Pratt David A. (Aberdeen CA GB6) Lungard Robert (Portola Valley CA), Derivatized D-acyl pyridinium reagent.
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Daniloff Yuri (Mountain View CA) Robins Simon P. (Aberdeen CA GB6) Evans Brian J. (Santa Clara CA) Pratt David A. (Aberdeen CA GB6) Lungard Robert (Portola Valley CA), Derivatized pyridinoline reagent.
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Daniloff Yuri ; Robins Simon P.,GB6 ; Evans Brian J. ; Pratt David A.,GB6 ; Lungard Robert, Derivatized pyridinoline reagent.
상세보기
Anderson Hugh B. (Toronto CAX), Device for performing enzyme immunoassays.
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Chandler Howard M. (Orton CAX), Device for performing immunochemical assays.
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Liotta Lance A. (5621 Sonoma Rd. Bethesda MD 20817), Enzyme immunoassay with two-zoned device having bound antigens.
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Rovelli Cesare (Milan ITX) Brustolin Domenico (Milan ITX) Piro Paola (Milan ITX), Immunodiagnostic device and method.
상세보기
Shigekawa Brian L. (Chapel Hill NC) Hsieh Yung-Ao (Cary NC), Ligand gold bonding.
상세보기
Hatch Robert P., Method for the synthesis of deoxypyridinoline.
상세보기
Citri Nathan (Jerusalem ILX), Method, reagent mixture and kit for determining the presence of bacterial or somatic cells in urine.
상세보기
Bohn Hans (Marburg ; Marbach DT) Stutzinger Ferdinand (Waldstetten DT), Pregnancy-specific b1-glycoprotein and process for isolating it.
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이 특허를 인용한 특허 (5)

Di Tullio, Katrina; Collier, G. Bruce; Campbell, John Lewis Emerson, Electrochemical methods and devices for amending urine samples for immunosensor detection.
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Di Tullio, Katrina; Collier, G. Bruce; Campbell, John Lewis Emerson, Electrochemical methods and devices for amending urine samples for immunosensor detection.
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Di Tullio, Katrina; Collier, G. Bruce; Campbell, John Lewis Emerson, Electrochemical methods and devices for amending urine samples for immunosensor detection.
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Di Tullio, Katrina; Collier, G. Bruce; Campbell, John Lewis Emerson, Electrochemical methods and devices for amending urine samples for immunosensor detection.
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Krauth, Gary H.; Ledden, David J., Native analyte as a reference in lateral flow assays.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

연합인증

Formulation for reducing urea effect for immunochromatography assays using urine samples 원문보기