초록 ▼

The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to posit

The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.

대표청구항 ▼

1. A bleaching composition comprising:(a) an effective amount of a protease variant wherein said protease variant includes a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliq

1. A bleaching composition comprising:(a) an effective amount of a protease variant wherein said protease variant includes a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin;(b) a bleaching agent which either is an organic peroxyacid or is a combination of a bleach activator and a peroxygen compound capable of yielding hydrogen peroxide that can react with the activator to form an organic peroxyacid in situ in a bleaching solution formed from the composition; and(c) one or more cleaning adjunct materials further wherein said protease variant includes substitutions of the amino acid residues at one or more positions selected from the group consisting of:1) position 62 and at one or more of the following positions 103, 104, 109, 159, 213, 232, 236, 245, 248 and 252;2) position 212 and at one or more of the following positions 12, 98, 102, 103, 104, 159, 232, 236, 245, 248 and 252;3) position 230 and at one or more of the following positions 68, 103, 104, 159, 232, 236 and 245;4) position 232 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 212, 213, 217, 230, 236, 245, 248, 252, 257, 260, 270 and 275;5) position 232 and at one or more of the following positions 103, 104, 236 and 245;6) positions 232 and 103 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 212, 213, 217, 230, 236, 245, 248, 252, 257, 260, 270 and 275;7) positions 232 and 104 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 212, 213, 217, 230, 236, 245, 248, 252, 257, 260, 270 and 275;8) positions 232 and 236 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 212, 213, 217, 230, 236, 245, 248, 252, 257, 260, 270 and 275;9) positions 232 and 245 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 212, 213, 217, 230, 236, 245, 248, 252, 257, 260, 270 and 275;10) positions 232, 103, 104, 236 and 245 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 212, 213, 217, 230, 236, 245, 248, 252, 257, 260, 270 and 275;11) position 252 and at one or more of the following positions 12, 61, 62, 68, 97, 98, 101, 102, 103, 104, 109, 13 0, 131, 159, 183, 185, 210, 212, 213, 217, 230, 236, 245, 248, and 270;12) position 252 and at one or more of the following positions 103, 104, 236 and 245;13) positions 252 and 103 and at one or more of the following positions 12, 61, 62, 68, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 210, 212, 213, 217, 232, 236, 245, 248, and 270;14) positions 252 and 104 and at one or more of the following positions 12, 61, 62, 68, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 210, 212, 213, 217, 232, 236, 245, 248, and 270;15) positions 252 and 236 and at one or more of the following positions 12, 61, 62, 68, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 210, 212, 213, 217, 232, 236, 245, 248, and 270;16) positions 252 and 245 and at one or more of the following positions 12, 61, 62, 68, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 210, 212, 213, 217, 232, 236, 245, 248, and 270;17) positions 252, 103, 104, 236 and 245 and at one or more of the following positions 12, 61, 62, 68, 97, 98, 101, 102, 103, 104, 109, 130, 131, 159, 183, 185, 210, 212, 213, 217, 232, 236, 245, 248, and 270; and18) position 257 and at one or more of the following positions 68, 103, 104, 205, 209, 210, 232, 236, 245 and 275. 2. The bleaching composition according to claim 1 wherein said protease variant is derived from a Bacillus subtilisin. 3. The bleaching composition according to claim 1 wherein said protease variant includes substitutions of the amino acid residues at position 103 and at one or more of the following positions 236 and 246 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 and 275 or at positions 103 and 245 and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 104, 109, 130, 131, 159, 170, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 222, 230, 232, 248, 252, 257, 260, 261, 270 and 275, and at one or more of the following positions 12, 61, 62, 68, 76, 97, 98, 101, 102, 104, 109, 130, 131, 159, 183, 185, 205, 209, 210, 211, 212, 213, 215, 217, 230, 232, 248, 252, 257, 260, 270 and 275. 4. The bleaching composition according to claim 1 wherein said protease variant includes a substitution set selected from the group consisting of:12/102/103/104/159/212/232/236/245/248/252;12/76/103/104/130/170/185/222/243/245;12/76/103/104/130/222/245/261; 12/76/103/104/222/245;12/76/103/104/222/245;61/68/103/104/159/232/236/245/248/252; 62/103/104/159/213/232/236/245/248/252;62/103/104/109/159/213/232/236/245/248/252; 62/103/104/159/232/236/245/248/252;62/101/103/104/159/212/213/232/236/245/248/252;62/103/104/130/159/213/232/236/245/248/252;68/103/104/159/232/236/245/248/252/270;68/103/104/159/185/232/236/245/248/252; 68/103/104/159/210/232/236/245/248/252;68/103/104/159/185/210/232/236/245/248/252;68/103/104/159/213/232/236/245/248/252;68/103/104/159/230/232/236/245; 68/76/103/104/159/209/232/236/245;68/103/104/232/236/245/248/257/275; 68/103/104/213/232/236/245/248/252;68/103/104/159/232/236/245/248/252; 68/103/104/159/209/232/236/245;68/76/103/104/159/236; 68/76/103/104/159/236/245;68/76/103/104/159/232/236/245; 68/103/104/159/232/236/245/252;68/103/104/159/232/236/245; 68/103/104/159/232/236/245/257;68/76/103/104/159/211/232/236/245; 68/76/103/104/159/215/232/236/245;68/103/104/159/210/232/236/245; 68/103/104/159/213/232/236/245/260;68/76/103/104/159/213/232/236/245/260; 68/103/104/159/236;68/76/103/104/159/210/232/236/245/260; 68/103/104/159/236/245;68/103/104/159/183/232/236/245/248/252; 68/76/103/104/159/236/245;68/103/104/232/236/245/257/275; 68/103/104/159/213/232/236/245;76/103/222/245; 76/103/159/232/236/245;76/103/104/159/213/232/236/245/260; 76/103/104/159;76/103/104/131/159/232/236/245/248/252; 76/103/104/222/245;97/103/104/159/232/236/245/248/252;98/102/103/104/159/212/232/236/245/248/252; 98/103/104/159/232/236/245/248/25 2;101/103/104/159/232/236/245/248/252; 102/103/104/159/232/236/245/248/252;103/104/159/232/236/245; 103/104/159/232/236/245/248/252;103/104/159/205/209/232/236/245/257 103/104/159/232/245/248/252;103/104/159/205/209/210/232/236/245/257; 103/104/159/213/232/236/245/248/252;103/104/159/217/232/236/245/248/252; 103/104/130/159/232/236/245/248/252;103/104/159/230/236/245; 103/104/159/236/245;103/104/159/248/252/270; 103/104/131/159/232/236/245/248/252;103/104/159/205/209/232/236/245; and 103/104/159/232/236/245/257. 5. The bleaching composition according to claim 4 wherein said protease variant includes a substitution set selected from the group consisting of:12R/76D/103A/104T/130T/222S/245R;12R/76D/103A/104I/222S/245R;12R/102A/103A/104I/159D/212G/232V/236H/245R/248D/252K;12R/76D/103A/104T/130G/222S/245R/261D;12R/76D/103A/104T/130G/170S/185D/222S/243D/245R;61E/68A/103A/104I/159D/232V/236H/245R/248D/252K;62D/103A/104I/109R/159D/213R/232V/236H/245R/248D/252K;62D/103A/104I/159D/213R/232V/236H/245R/248D/252K;62D/103A/104I/159D/232V/236H/245R/248D/252K;62D/103A/104I/130G/159D/213R/232V/236H/245R/248D/252K;62D/101G/103A/104I/159D/212G/213R/232V/236H/245R/248D/252K;68A/76D/103A/104I/159D/213R/232V/236H/245R/260A;68A/103A/104I/159D/236H;68A/103A/104I/159D/236H/245R;68A/76D/103A/104I/159D/210I/232V/236H/245R/260A;68A/103A/104I/159D/183D/232V/236H/245R/248D/252K;68A/103A/104I/159D/209W/232V/236H/245R;68A/76D/103A/104I/159D/211R/232V/236H/245R;68A/76D/103A/104I/159D/215R/232V/236H/245R;68A/103A/104I/159D/213R/232V/236H/245R/260A;68A/76D/103A/104I/159D/236H;68A/76D/103A/104I/159D/236H/245R;68A/76D/103A/104I/159D/232V/236H/245R;68A/103A/104I/159D/232V/236V/245R/252K;68A/103A/104I/159D/232V/236H/245R;68A/103A/104I/159D/232V/236H/245R/257V;68A/103A/104I/159D/185D/232V/236H/245R/248D/252K;68A/103A/104I/159D/210L/232V/236H/245R/248D/252K;68A/103A/104I/159D/185D/210L/232V/236H/245R/248D/252K;68A/103A/104I/159D/213E/232V/236H/245R/248D/252K;68A/103A/104I/159D/230V/232V/236H/245R;68A/76D/103A/104I/159D/209W/232V/236H/245R;68A/103A/104I/232V/236H/245R/248D/257V/275H;68A/103A/104I/232V/236H/245R/257V/275H;68A/103A/104I/213E/232V/236H/245R/248D/252K;68A/103A/104I/159D/232V/236H/245R/248D/252K;68A/103A/104I/159D/210I/232V/236H/245R;68A/103A/104I/159D/210L/232V/236H/245R;68A/103A/104I/159D/213G/232V/236H/245R;68A/103A/104I/159D/232V/236H/245R/248D/252K/270A;76D/103A/222S/245R;76D/103A/104I/159D/232V/236H/245R;76D/103A/104I/159D;76D/103A/104I/222S/245R;76D/103A/104I/159D/232V/236V/245H/248R/252K;76D/103A/104I/159D/213R/232V/236H/245R/260A;97E/103A/104I/159D/232V/236H/245R/248D/252K;98L/103A/104I/159D/232V/236H/245R/248D/252K;98L/102A/103A/104I/159D/212G/232V/236H/245R/248D/252K;101G/103A/104I/159D/232V/236H/245R/248D/252K;102A/103A/104I/159D/232V/236H/245R/248D/252K;103A/104I/159D/232V/236H/245R/248D/252K;103A/104I/159D/213R/232V/236H/245R/248D/252K;103A/104I/130G/159D/232V/236H/245R/248D/252K;103A/104I/159D/230V/236H/245R;103A/104I/159D/217E/232V/236H/245R/248D/252K;103A/104I/159D/236H/245R;103A/104I/159D/248D/252K/270V;103A/104I/159D/232V/236H/245R;103A/104I/159D/205I/209W/232V/236H/245R;103A/104I/159D/232V/236H/245R/257V;103A/104I/159D/205I/209W/232V/236H/245R/257V;103A/104I/131V/159D/232V/236H/245R/248D/252K;103A/104I/159D/205I/209W/210I/232V/236H/245R/257V; and103A/104I/159D/232V/245R/248D/252K. 6. The bleaching composition according to claim 1 wherein said bleaching agent is selected from the group consisting of:(i) an organic peroxyacid selected from the group consisting of organic peroxyacids of the formula:wherein R 1 is an alkyl, aryl, or alkaryl group containing from about 1 to about 14 carbon atoms, R 2 is an alkylene, arylene or alkarylene group containing from about 1 to about 14 carbon atoms, and R 5 is H or an alkyl, aryl, or alkaryl group containing from about 1 to about 10 carbon atoms; E-phthalimido peroxycaproic acids; and mixtures thereof; and(ii) a combination of a bleach activator and a peroxygen compound capable of yielding hydro gen peroxide that can react with the activator to form an organic peroxyacid in situ in a bleaching solution formed from the composition, wherein said bleach activator has the general formula: wherein R is an alkyl group containing from about 5 to about 18 carbon atoms wherein the longest linear alkyl chain extending from and including the carbonyl carbon contains from about 6 to about 10 carbon atoms and L is a leaving group, the conjugate acid of which has a pKa in the range of from about 4 to about 13;said bleach activator having the general formula: or mixtures thereof, wherein R 1 is an alkyl, aryl, or alkaryl group containing from about 1 to about 14 carbon atoms, R 2 is an alkylene, arylene or alkarylene group containing from about 1 to about 14 carbon atoms, R 5 is H or an alkyl, aryl, or alkaryl group containing from about 1 to about 10 carbon atoms, and L is a leaving group. 7. The bleaching composition according to claim 6 wherein R 1 is an alkyl group containing from about 7 to about 10 carbon atoms, R 2 contains from about 4 to about 5 carbon atoms, and wherein L to selected from the group consisting of:wherein R 3 is an alkyl chain containing from about 1 to about 8 carbon atoms, and Y is —SO 3 —M + or —CO 2 − M + wherein M is sodium or potassium. 8. The bleaching composition according to claim 1 wherein said bleaching agent comprises a bleach activator selected from the group consisting of tetraacetyl ethylene diamine (TAED), benzoylcaprolactam (BzCL), 4-nitrobenzoylcaprolactam, 3-chlorobenzoyl-caprolactam, benzoyloxybenzenesulphonate (BOBS), nonanoyloxybenzenesulphonate (NOBS), phenyl benzoate (PhBz), decanoyloxybenzenesulphonate (C 10 -OBS), benzoylvalerolactam (BZVL), octanoyloxybenzenesulphonate (C 8 -OBS), perhydrolyzable esters, 4-[N-(nonaoyl) amino hexanoyloxy]-benzene sulfonate sodium, salt (NACA-OBS), lauryloxybenzenesulphonate (LOBS or C 12 -OBS), 10-undecenoyloxybenzenesulfonate (UDOBS or C 11 -OBS with unsaturation in the 10 position), and decanoyloxybenzoic acid (DOBA) and mixtures thereof, and further optionally comprises a bleach catalyst, preferably 3-(3,4-dihydroisoquinolinium)propane sulfonate. 9. The bleaching composition according to claim 1 wherein said bleaching agent comprises at least about 0.1% by weight of the bleaching agent of a peroxygen bleaching compound capable of yielding hydrogen peroxide in an aqueous liquor and at least 0.1% by weight of the bleaching agent of one or more bleach activators, wherein said bleach activators are members selected from the group consisting of:a) a bleach activator at the general formula: or mixtures thereof, wherein R 1 is an alkyl, aryl, or alkaryl group containing from about 1 to about 14 carbon atoms, R 2 is an alkylene, arylene or alkarylene group containing from about 1 to about 14 carbon atoms, R 5 is H or an alkyl, aryl, or alkaryl group containing from about 1 to about 10 carbon atoms, and L is a leaving group:b) a benzoxazin-type bleach activator of the formula: wherein R1 H, alkyl, alkaryl, aryl, arylalkyl, and wherein R2, R3, R4, and R5 may be the same or different substituents selected from H, halogen, alkyl, alkenyl, aryl, hydroxyl, alkoxyl, amino, alkylamino, —COOR 6 , wherein R 6 is H or an alkyl group and carbonyl functions;c) a N-acyl caprolactam bleach activator of the formula: wherein R 6 is H or an alkyl, aryl, alkoxyaryl, or a group containing from 1 to 12 carbons; andd) mixture of a), b) and c). 10. The bleaching composition according to claim 1 wherein the corresponding carboxylic acid of the organic peroxyacid bleaching agent has a Hydrophillic-Lipophilic Balance value within the range of from about 3 to about 6.5. 11. The bleaching composition according to claim 1 wherein said cleaning adjunct materials are selected from the group consisting of surfactants, solvents, buffers, enzymes, soil release agents, clay soil removal agents, di spersing agents, brighteners, suds suppressors, fabric softeners, suds boosters, enzyme stabilizers, builders, other bleaching agents, dyes, perfumes, chelants and mixtures thereof. 12. The bleaching composition according to claim 11 wherein said cleaning adjunct materials comprise at least one detersive surfactant. 13. The bleaching composition according to claim 11 wherein the cleaning adjunct materials comprise at least about 0.1% surfactant by weight of the composition, said surfactant comprising materials selected from the group consisting of alkyl benzene sulfonates, primary alkyl sulfates, secondary alkyl sulfates, alkyl alkoxy sulfates, alkyl alkoxy carboxylates, alkyl polyglycosides and their corresponding sulfated polyglycosides, alpha-sulfonated fatty acid esters, alkyl and alkyl phenol alkoxylates, betaines and sulfobetaines, amine oxides, N-methyl gluamides, nonoinic primary alcohol ethoxylates, nonionic primary alcohol mixed ethoxy/propoxy, and mixtures thereof. 14. The bleaching composition according to claim 13 further comprising at least about 6% builder selected from the group consisting of zeolites, polycarboxylates, layered silicates, phosphates, and mixtures thereof. 15. The bleaching composition according to claim 11 wherein said cleaning adjunct materials comprise at least one detersive enzyme selected from the group consisting of cellulases, lipases, amylases, phospholipases, other proteases, peroxidases and mixtures thereof. 16. The bleaching composition according to claim 1 wherein said bleaching composition is a fabric bleaching composition, in the form of a liquid, granule, tablet, powder or bar, comprising at least about 5% surfactant and at least about 5% builder by weight of the composition. 17. The bleaching composition according to claim 1 wherein said bleaching composition is a fabric bleaching composition comprising:(a) from about 0.0001% to about 10% by weight of said protease variant;(b) from about 0.5% to about 20% by weight of said bleaching agent;(c) at least about 5% by weight of a surfactant selected from the group consisting of alkyl benzene sulfonates, primary alkyl sulfates, secondary alkyl sulfates, alkyl alkoxy sulfates, alkyl alkoxy carboxylates, alkyl polyglycosides and their corresponding sulfated polyglycosides, alpha-sulfonated fatty acid esters, alkyl and alkyl phenol alkoxylates, betaines and sulfobetaines, amine oxides, N-methyl glucamides, nonionic primary alcohol ethoxylates, nonionic primary alcohol mixed ethoxy/propoxy, and, mixtures thereof; and(d) at least about 5% by weight of a builder selected from the group consisting of zeolites, polycarboxylates, layered silicates, phosphates, and mixtures thereof. 18. The bleaching composition according to claim 17 is in the form of a concentrated granular fabric bleaching composition comprising at least about 15% surfactant. 19. A method for cleaning fabric, said method comprising contacting a fabric in need of cleaning with a bleaching composition according to claim 16 or 17. 20. The bleaching composition according to claim 1 wherein said bleaching composition is a dishwashing bleaching composition, in the form of a liquid, granule, powder or tablet, comprising:(a) from about 0.0001% to about 10% by weight of the dishwashing bleaching composition of said protease variant;(b) from about 0.5% to about 20% by weight of the dishwashing bleaching composition of said bleaching agent(c) from about 0.1% to about 10% by weight of the dishwashing bleaching composition of a surfactant. 21. A method for cleaning dishes, said method comprising contacting a dish in need of cleaning with a bleaching composition according to claim 20. 22. The bleaching composition according to claim 1 wherein said bleaching composition is a personal cleansing composition comprising:(a) from about 0.001% to about 5%, by weight of the personal cleansing composition of a protease variant wherein said proteases variant includes a substitution of an amino acid r esidue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 66, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 148, 147, 158, 159, 160, 168, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 268, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 218, 217, 218, 222, 280, 265 or 274 of Bacillus amyloliquefaciens subtillsin;(b) from about 0.5% to about 20% by weight of the personal cleansing composition of a bleaching agent which either is an organic peroxyacid or is a combination of a bleach activator and a peroxygen compound capable of yielding hydrogen peroxide that can react with the activator to form an organic peroxyacid in situ in a bleaching solution formed from the composition; and(c) from about 0.1% to about 95% by weight of the personal cleansing composition of a surfactant system comprising one or more surfactants selected from the group consisting of anionic carboxylates, amine oxides, alkyl glucosides, glucose amides, alkyl sulfates, alkyl ether sulfates, acyl isethionates, alkyl sulfosuccinates, alkyl phosphate esters, ethoxylated phosphate esters, alkyl glyceryl ether sulfonates and mixtures thereof,(d) optionally, from about 0.05% to about 50% by weight of the personal cleaning composition of an enzyme stabilizer. 23. The bleaching composition according to claim 22 wherein said surfactant is soap at a level of at least about 2% by weight of the bleaching composition. 24. The bleaching composition according to claim 23 wherein the ratio of soap to protease variant is from about 2,000:1 to about 8:1. 25. A method for personal cleansing, said method comprising contacting a part of the human or lower animal body in need of cleaning with a bleaching composition according to claim 22. 26. A method for pretreating a fabric in need of cleaning, said method comprising contacting said fabric prior to washing said fabric with an aqueous solution containing a surfactant with a bleaching composition according to claim 16 or 17. 27. The cleaning composition according to claim 1 wherein said protease variant includes a substitution set selected from the group consisting of:12/102/103/104/159/212/232/236/245/248/252;61/68/103/104/159/232/236/245/248/252;62/103/104/130/159/213/232/236/245/248/252;62/103/104/159/232/236/245/248/252;62/103/104/109/159/213/232/236/245/248/252; 62/103/104/159/232/236/245/248/252;62/101/103/104/159/212/213/232/236/245/248/252;68/103/104/159/232/236/245/248/252/270;68/103/104/159/185/232/236/245/248/252; 68/103/104/159/210/232/236/245/248/252;68/103/104/159/185/210/232/236/245/248/252;68/103/104/159/213/232/236/245/248/252;68/103/104/159/230/232/236/245; 68/78/103/104/159/209/232/236/245;68/103/104/232/236/245/248/257/275; 68/103/104/213/232/236/245/248/252;68/103/104/159/232/236/245/248/252; 68/103/104/159/209/232/236/245;68/76/103/104/159/232/236/245; 68/103/104/159/232/236/245/ 252;68/103/104/159/232/236/245; 68/103/104/159/232/236/245/257;68/76/103/104/159/211/232/236/245; 68/76/103/104/159/215/232/236/245;68/103/104/159/210/232/236/245; 68/103/104/159/213/232/236/245/260;68/76/103/104/159/213/232/236/245/260; 68/76/103/104/159/210/232/236/245/260;68/103/104/159/183/232/236/245/248/252; 68/103/104/232/236/245/257/275;68/103/104/159/213/232/236/245; 76/103/104/159/232/236/245;76/103/104/159/213/232/236/245/260; 76/103/104/131/159/232/236/245/248/252;97/103/104/159/232/236/245/248/252; 98/103/104/159/232/236/245/248/252;98/102/103/104/159/212/232/236/245/248/252; 101/103/104/159/232/236/245/248/252;102/103/104/159/232/236/245/248/252; 103/104/159/232/236/245;103/104/159/248/252/270; 103/104/159/232/236/245/248/252;103/104/159/205/209/232/236/245/257 103/104/159/232/245/248/252;103/104/159/205/209/210/232/236/245/257; 103/104/159/213/232/236/245/248/252;103/104/159/217/232/236/245/248/252; 103/104/130/159/232/236/245/248/252;103/104/131/159/232/236/245/248/252; 103/104/159/205/209/232/236/245; and103/104/159/232/236/245/257. 28. The cleaning composition according to claim 27 wherein said protease variant includes a substitution set selected from the group consisting of:12R/102A/103A/104I/159D/212G/232V/236H/245R/248D/252K;61E/68A/103A/104I/159D/232V/236H/245R/248D/252K;62D/103A/104I/109R/159D/213R/232V/236H/245R/248D/252K;62D/103A/104I/159D/213R/232V/236H/245R/248D/252K;62D/103A/104I/159D/232V/236H/245R/248D/252K;62D/103A/104I/130G/159D/213R/232V/236H/245R/248D/252K;62D/101G/103A/104I/159D/212G/213R/232V/236H/245R/248D/252K;68A/76D/103A/104I/159D/213R/232V/236H/245R/260A;68A/76D/103A/104I/159D/210I/232V/236H/245R/260A;68A/103A/104I/159D/183D/232V/236H/245R/248D/252K;68A/103A/104I/159D/209W/232V/236H/245R;68A/76D/103A/104I/159D/211R/232V/236H/245R;68A/76D/103A/104I/159D/215R/232V/236H/245R;68A/103A/104I/159D/236H/245R/260A;68A/76D/103A/104I/159D/232V/236H/245R;68A/103A/104I/159D/232V/236H/245R/252K;68A/103A/104I/159D/232V/236H/245R;68A/103A/104I/159D/232V/236H/245R/257V;68A/103A/104I/159D/185D/232V/236H/245R/248D/252K;68A/103A/104I/159D/210R/232V/236H/245R/248D/252K;68A/103A/104I/159D/185D/210L/232V/236H/245R/248D/252K;68A/103A/104I/159D/213E/232V/236H/245R/248D/252K;68A/103A/104I/159D/230V/232V/236H/245R;68A/76D/103A/104I/159D/209W/232V/236H/245R;68A/103A/104I/232V/236H/245R/248D/257V/275H;68A/103A/104I/232V/236H/245R/257V/275H;68A/103A/104I/213E/232V/236H/245R/248D/252K;68A/103A/104I/159D/232V/236H/245R/248D/252K;68A/103A/104I/159D/210I/232V/236H/245R;68A/103A/104I/159D/210L/232V/236H/245R;68A/103A/104I/159D/213E/232V/236H/245R;68A/103A/104I/159D/232V/236H/245R/248D/252K/270A;76D/103A/104I/159D/232V/236H/245R;76D/103A/104I/131V/159D/232V/236V/245R/248D/252K;76D/103A/104I/159D/213R/232V/236H/245R/260A;97E/103A/104I/159D/232V/236H/245R/248D/252K;98L/103A/104I/159D/232V/236H/245R/248D/252K;98L/102A/103A/104I/159D/212G/232V/236H/245R/248D/252K;101G/103A/104I/159D/232V/236H/245R/248D/252K;102A/103A/104I/159D/232V/236H/245R/248D/252K;103A/104I/159D/232V/236H/245R/248D/252K;103A/104I/159D/213R/232V/236H/245R/248D/252K;103A/104I/130G/159D/232V/236H/245R/248D/252K;103A/104I/159D/217E/232V/236H/245R/248D/252K;103A/104I/159D/248D/252K/270V;103A/104I/159D/232V/236H/245R;103A/104I/159D/205I/209W/232V/236H/245R;103A/104I/159D/232V/236H/245R/257V;103A/104I/159D/205I/209W/232V/236H/245R/257V;103A/104I/131V/159D/232V/236H/245R/246D/252K;103A/104I/159D/205I/209W/210I/232V/236H/245R/257V; and103A/104I/159D/232V/245R/248D/252K. 29. The bleaching composition according to claim 1 wherein said bleaching agent is selected from the group consisting of:(i) an organic peroxyacid selected from the group consisting of organic peroxyacids of the formula: wherein R 1 is an alkyl, aryl, or alkaryl group containing from about 1 to about 14 carbon atoms, R 2 is an alkylene, arylene or alkarylene group containing from about 1 to about 14 carbon atoms, and R 5 is H or an alkyl, aryl, or alkaryl group containing from about 1 to about 10 carbon atoms; E-phthalimido peroxycaproic acids; and mixtures thereof; and(ii) a combination of a bleach activator and a peroxygen compound capable of yielding hydrogen peroxide that can react with the activator to form an organic peroxyacid in situ in a bleaching solution formed from the composition, wherein said bleach activator the general formula: wherein R is an alkyl group containing from about 5 to about 18 carbon atoms wherein the longest linear alkyl chain extending from and including the carbonyl carbon contains from about 6 to about 10 carbon atoms and L is a leaving group, the conjugate acid of which has a pKa in the range of from about 4to about 13;said bleach activator having the general formula: or mixtures thereof, wherein R 1 is an alkyl, aryl, or alkaryl group containing from about 1 to about 14 carbon atoms, R 2 is an alkylene, arylene or alkarylene group containing from about 1 to about 14 carbon atoms, R 5 is H or an alkyl, aryl, at alkaryl group containing from about 1 to about 10 carbon atoms, and L is a leaving group. 30. The bleaching composition according to claim 29 wherein R 1 is an alkyl group containing from about 7 to about 10 carbon atoms, R 2 contains from about 4 to about 5 carbon atoms, and wherein L is selected from the group consisting of;wherein R 3 is an alkyl chain containing from about 1 to about 6 carbon atoms, and Y is —SO 3 + or —CO 2 − M + wherein M is sodium or potassium. 31. The bleaching composition according to claim 1 wherein said bleaching agent comprises a bleach activator selected from the group consisting of tetraacetyl ethylene diamine (TAED), benzoylcaprolactam (BzCL), 4-nitrobenzoylcaprolactam, 3-chlorobenzoylcaprolactam, benzoyloxybenzenesulphonate (BOBS), nonanoyloxybenzenesulphonate (NOBS), phenyl benzoate (PhBz), decanoyloxybenzenesulphonate (C 10 -OBs), benzoylvalerolactam (BZVL), octanoyloxybenzenesulphonate, (C 8 -OBS), perhydrolyzable esters, 4-[N-(nonaoyl)amino hexanoyloxy]-benzene sulfonate sodium salt (NACA-OBS), lauryloxybenzenesulphonate (LOBS or C 12 -OBS), 10-undecenoyloxybenzenesulfonate (UDOBS or C 11 -OBS with unsaturation in the 10 position), and decanoyloxybenzolo acid (DOBA) and mixtures thereof, and further optionally comprises a bleach catalyst. 32. The bleaching composition according to claim 1 wherein said bleaching agent comprises at least about 0.1% by weight of the bleaching agent of a peroxygen bleaching compound capable of yielding hydrogen peroxide in an aqueous liquor and at least 0.1% by weight of the bleaching agent of one or more bleach activator, wherein said bleach activators are members selected from the group consisting of:a) a bleach activator of the general formula: or mixtures thereof, wherein R 1 is an alkyl, aryl, or alkaryl group containing from about 1 to about 14 carbon atoms, R 2 is an alkylene, arylene or alkarylene group containing from about 1 to about 14 carbon atoms, R 5 is H or an alkyl, aryl, or alkaryl group containing from about 1 to about 10 carbon atoms, and L is a leaving group;b) a benzoxazin-type bleach activator of the formula: wherein R 1 is H, alkyl, alkaryl, aryl, arylalkyl, and wherein R2, R3, R4, and R5 may be the same or different substituents selected from H, halogen, alkyl, alkenyl, aryl, hydroxyl, alkoxyl, amino, alkylamino, —COOR 6 , wherein R 6 is H or an alkyl group and carbonyl functions;c) a N-acyl caprolactam bleach activator of the formula: wherein R 6 is H or an alkyl, aryl, alkoxyaryl, or alkaryl group containing from 1 to 12 carbons; andd) mixtures of a), b) and c). 33. The bleaching composition according to claim 1 wherein the corresponding carboxylic acid of the organic peroxyacid bleaching agent has a Hydrophilic-Lipophillic Balance value within the range of from about 3 to about 6.5. 34. The bleaching composition according to claim 1 wherein said cleaning adjunct materials are selected fro m the group consisting of surfactants, solvents, buffers, enzymes, soil release agents, clay removal agents, dispersing agents, brighteners, suds suppressors, fabric softeners, suds boosters, enzyme stabilizers, builders, other bleaching agents, dyes, perfumes, chelants and mixtures thereof. 35. The bleaching composition according to claim 34 wherein said cleaning adjunct materials comprise at least one detersive surfactant. 36. The bleaching composition according to claim 34 wherein the cleaning adjunct materials comprise at least about 0.1% surfactant by weight of the composition, said surfactant comprising materials selected from the group consisting of alkyl benzene sulfonates, primary alkyl sulfates, secondary alkyl sulfates, alkyl alkoxy sulfates, alkyl alkoxy carboxylates, alkyl polyglycosides and their corresponding sulfated polyglycosides, alpha-sulfonated fatty acid esters, alkyl and alkyl phenol alkoxylates, betaines and sulfobetaines, amine oxides, N-methyl glucamides, nonionic primary alcohol ethoxylates, nonionic primary alcohol mixed ethoxy/propoxy, and mixtures thereof. 37. The bleaching composition according to claim 36 further comprising at least about 5% builder selected from the group consisting of zeolites, polycarboxylates, layered silicates, phosphates, and mixtures thereof. 38. The bleaching composition according to claim 34 wherein said cleaning adjunct materials comprise at least one detersive enzyme selected from the group consisting of cellulases, lipases, amylases, phospholipases, other proteases, peroxidases and mixtures thereof. 39. The bleaching composition according to claim 1 wherein said bleaching composition is a fabric bleaching composition, preferably in the form of a liquid, granule, tablet, powder or bar, comprising at least about 5% surfactant and at least about 5% builder by weight of the composition. 40. The bleaching composition according to claim 1 wherein said bleaching composition is a fabric bleaching composition comprising:(a) from about 0.0001% to about 10% by weight of said protease variant;(b) from about 0.5% to about 20% by weight of said bleaching agent;(c) at least about 5% by weight of a surfactant selected from the group consisting of alkyl benzene sulfonates, primary alkyl sulfates, secondary alkyl sulfates, alkyl alkoxy sulfates, alkyl alkoxy carboxylates, alkyl polyglycosides and their corresponding sulfated polyglycosides, alpha-sulfonated fatty add esters, alkyl and alkyl phenol alkoxylates, betaines and sulfobetaines, amine oxides, N-methyl glucamides, nonionic primary alcohol ethoxylates, nonionic primary alcohol mixed ethoxy/propoxy, and mixtures thereof; and(d) at least about 6% by weight of a builder selected from the group consisting of zeolites, polycarboxylates, layered silicates, phosphates, and mixtures thereof. 41. The bleaching composition according to claim 40 in the form of a concentrated granular fabric bleaching composition comprising at least about 15% surfactant. 42. A method for cleaning fabric, said method comprising contacting a fabric in need of cleaning with a bleaching composition according to claims 39 or 40 . 43. The bleaching composition according to claim 1 wherein said bleaching composition is a dishwashing bleaching composition, in the form of a liquid, granule, powder or tablet, comprising:(a) from about 0.0001% to about 10% by weight of the dishwashing bleaching composition of said protease variant;(b) from about 0.5% to about 20% by weight of the dishwashing bleaching composition of said bleaching agent(c) from about 0.1% to about 10% by weight of the dishwashing bleaching composition of a surfactant. 44. A method for cleaning dishes, said method comprising contacting a dish in need of cleaning with a bleaching composition according to claim 43. 45. A personal cleansing composition comprising:(a) from about 0.001% to about 5% by weight of the personal cleansing composition of a protease variant wherein said protease variant includes a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin;(b) from about 0.5% to about 20% by weight of the personal cleansing composition of a bleaching agent which either is an organic peroxyacid or is a combination of a bleach activator and a peroxygen compound capable of yielding hydrogen peroxide that can react with the activator to form an organic peroxyacid in situ in a bleaching solution formed from the composition; and(c) from about 0.1% to about 95% by weight of the personal cleansing composition of a surfactant system comprising one or more surfactants selected from the group consisting of anionic carboxylates, amine oxides, alkyl glucosides, glucose amides, alkyl sulfates, alkyl ether sulfates, acyl isothiaonates, alkyl sulfosuccinates, alkyl phosphate esters, ethoxylated phosphate easters, alkyl glyceryl ether sulfonates and mixtures thereof; and(d) optionally, from about 0.05% to about 50% by weight of the personal cleansing composition of an enzyme stabilizer. 46. The bleaching composition according to claim 45 wherein said surfactant is soap at a level of at least about 2% by weight of the bleaching composition. 47. The bleaching composition according to claim 46 wherein the ratio of soap to protease variant is from about 2,000:1 to about 8:1. 48. A method for personal cleansing, said method comprising contacting a part of the human or lower animal body in need of cleaning with a bleaching composition according to claim 45. 49. A method for pretreating a fabric in need of cleaning, said method comprising contacting said fabric prior to washing said fabric with an aqueous solution containing a surfactant with a bleaching composition according to claim 39 or 40.