IPC분류정보
국가/구분 |
United States(US) Patent
등록
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국제특허분류(IPC7판) |
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출원번호 |
US-0922349
(2001-08-03)
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발명자
/ 주소 |
- John, Havens R.
- Winger, Theodore M.
- Krotz, Jain
- Dan, Smolko
- Onofrey, Thomas J.
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 |
피인용 횟수 :
14 인용 특허 :
73 |
초록
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Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability
Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability to apply an electronic bias to the electrodes of the microchip while preventing permeation layer delaminating from the electrode surface.
대표청구항
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1. A device comprising a plurality of microlocations:wherein the microlocations each comprise an underlying working microelectrode on a substrate, wherein the microelectrode comprises platinum silicide (PtSi), wherein at least some of the microelectrodes are covered by a permeation layer, further wh
1. A device comprising a plurality of microlocations:wherein the microlocations each comprise an underlying working microelectrode on a substrate, wherein the microelectrode comprises platinum silicide (PtSi), wherein at least some of the microelectrodes are covered by a permeation layer, further wherein at at least one microlocation the permeation layer is covalently attached to the electrode by linker moieties, and wherein the covalent attachment between the electrode and the linker and the permeation layer material is stable at a current density of at least 0.10 nA/μm2. 2. The device of claim 1 wherein the permeation layer comprises a material selected from the group consisting of an inorganic sol-gel, a synthetic polymer hydrogel, and a carbohydrate hydrogel.3. The device of claim 1 wherein the linker has the formula wherein:X is selected from the group consisting of acrylate, methacrylate, acrylamide, methacrylamide, allyl, vinyl, acetyl, amine, substituted amine, epoxy and thiol; SPACER is selected from the group consisting of alkyl, aryl, mono- or polyalkoxy, ethyleneglycol, polyethyleneglycol, mono- or polyalkylamine, mono- or polyamide, thioether derivatives, and mono- or polydisulfides; A and B are selected from the group consisting of Oxygen-R, Cl, Br, and an X-SPACER moiety, or any combination thereof, wherein R is H, alkyl, methyl, ethyl, propyl, isopropyl, and branched or linear alkyl of 4 to 10 carbon atoms; and C is a hydrolyzable moiety selected from the group consisting of Oxygen-R, Cl, and Br, wherein R is H, branched alkyl, methyl, ethyl, propyl, isopropyl, and branched or linear alkyl of 4 to 10 carbon atoms. 4. The device of claim 3 wherein the linker is selected from the group consisting of:H2NCH2CH2CH2CH2Si(OCH3)3), H2NCH2CH2NHCH2CH2CH2Si(OCH3)3, H2NCH2CH2CH2CH2CH2NHCH2CH2CH2Si(OCH3)3, CH2?C(CH3)COOCH2CH2CH2Si(OCH3)3, andCH2?CHCONHCH2CH2CH2Si(OC2H5)3. 5. The device of claim 1 wherein the permeation layer is a hydrogel comprising a material selected from the group consisting of: agarose, glyoxylagarose, acrylamide, methacrylamide, polyacrylamide, and other synthetic polymers.6. The device of claim 5 wherein the hydrogel comprises glyoxylagarose.7. The device of claim 5 wherein the hydrogel comprises polyacrylamide.8. The device of claim 1 wherein the electrode is a metal/silicide electrode selected from the group consisting of platinum suicide (PtSi), tungsten suicide (WSi), titanium silicide (TiSi), and gold suicide (AuSi).9. The device of claim 1 wherein the electrode is a metal/metal electrode selected from the group consisting of platinum/titanium (PtTi) and gold/titanium (AuTi).10. The device of claim 1 wherein the electrode is an organic electrode selected from the group consisting of poly(phenylene vinylene), polythiophene, and polyaniline.11. The device of claim 3 wherein the linker is an acrylate linker selected from the group consisting of:CH2?CHCOOCH2CH2CH2Si(OCH3)3, CH2?CHCOOCH2CH2CH2SiCl3, CH2?CHCOOCH2CH2CH2Si(CH3)(OCH3)2, CH2?CHCOOCH2CH2CH2Si(CH3)2(OCH3), CH2?CHCOOCH2CH2CH2Si(CH3)Cl2, andCH2?CHCOOCH2CH(OH)CH2NHCH2CH2CH2Si(OC2H5)3. 12. The device of claim 3 wherein the linker is a methacrylate linker selected from the group consisting of:CH2?C(CH3)COOCH2CH2CH2Si(OCH3)3, CH2?C(CH3)COOCH2CH2CH2SiCl3, CH2?C(CH3)COOCH2CH2CH2Si(CH3)(OCH3)2, CH2?C(CH3)COOCH2CH2CH2Si(CH3)2(OCH3), CH2?C(CH3)COOCH2CH2CH2Si(CH3)Cl2, andCH2?C(CH3)COOCH2CH(OH)CH2NHCH2CH2CH2Si(OC2H5)3. 13. The device of claim 3 wherein the linker is an acrylamide linker selected from the group consisting of;CH2?CHCONHCH2CH2CH2Si(OC2H5)3, CH2?CHCONHCH2CH2CH2SiCl3, CH2?CHCONHCH2CH2CH2Si(CH3)(OCH3)2, CH2?CHCONHCH2CH2CH2Si(CH3)2(OCH3), CH2?CHCONHCH2CH2CH2Si(CH3)Cl2, CH2?CHCONHCH2CH(OH)CH2NHCH2CH2CH2Si(OC2H5)3, andCH2?CHCONHCH2CH2CONHCH2CH2CONHCH2CH2CH2Si(OC2H5)3. 14. The device of claim 3 wherein the linker is a methacrylamide linker selected from the group consisting of:CH2?C(CH3)CONHCH2CH2CH2Si(OCH3)3, CH2?C(CH3)CONHCH2CH2CH2SiCl3, CH2?C(CH3)CONHCH2CH2CH2Si(CH3)(OCH3)2, CH2?C(CH3)CONHCH2CH2CH2Si(CH3)2(OCH3), CH2?C(CH3)CONHCH2CH2CH2Si(CH3)Cl2, andCH2?C(CH3)CONHCH2CH(OH)CH2NHCH2CH2CH2Si(OC2H5)3. 15. The device of claim 3 wherein the linker is an allyl derivative linker selected from the group consisting of:CH2?CHCH2NHCH2CH2CH2Si(OCH3)3, CH2?CHCH2SiH(OCH3)2, CH2?CHCH2Si(CH3)2Cl, CH2?CHCH2SiHCl2, andCH2?CHCH2Si(OCH3)3. 16. The device of claim 3 wherein the linker is an amino derivative linker selected from the group consisting of:H2NCH2CH2NHCH2CH2CH2Si(OCH3)3, H2NCH2CH2CH2CH2CH2CH2NHCH2CH2CH2Si(OCH3)3, H2NCH2CH2CH2Si(OCH3)3, andH2NCH2CH2CH2Si(OC2H5)3. 17. The device of claim 3 wherein the linker is an epoxy derivative linker selected from the group consisting of: 18. The device of claim 4 wherein the linker is H2NCH2CH2CH2Si(OCH3)3.19. The device of claim 4 wherein the linker is H2NCH2CH2NHCH2CH2CH2Si(OCH3)3.20. The device of claim 4 wherein the linker is CH2?C(CH3)COOCH2CH2CH2Si(OCH3)3.21. The device of claim 1 wherein the resulting covalent attachment between the electrode and the linker and the permeation layer material is stable at a current density of at least 0.2 nA/μm2.22. The device of claim 1 wherein the resulting covalent attachment between the electrode and the linker and the permeation layer material is stable at a current density of at least 0.4 nA/μm2.
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