[특허]Bone mineralization proteins, DNA, vectors expression systems

Bone mineralization proteins, DNA, vectors expression systems 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12N-015/63 C12N-015/85 C12N-015/86 C12N-015/87 C07H-021/02 C07H-021/04
출원번호	US-0986625 (2001-11-09)
발명자 / 주소	Hair, Gregory A. Boden, Scott D.
출원인 / 주소	Medtronic Sofamor Danek
대리인 / 주소	Baker, Donelson, Bearman, Caldwell &
인용정보	피인용 횟수 : 4 인용 특허 : 242

초록 ▼

The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

대표청구항 ▼

1. A method of inducing bone formation comprising transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein, wherein said nucleic acid molecule is SEQ ID NO: 22 or SEQ ID NO: 33.2. The method of claim 1, wherein the isolated nucleic acid molecule is in a vector.3. The method of claim 2, wherein the vector is an expression vector.4. The method of claim 3, wherein the vector is a plasmid.5. The method of claim 3, wherein the vector is a virus.6. The method of claim 5, wherein the virus is an adenovirus.7. The method of claim 5, wherein the virus is a retrovirus.8. The method of claim 1, wherein the osteogenic precursor cells are transfected ex vivo.9. The method of claim 1, wherein the osteogenic precursor cells are transfected in vivo by direct injection of the isolated nucleic acid molecule.10. The method of claim 1, wherein the LIM mineralization protein is HLMP-1 (SEQ ID NO: 10).11. The method of claim 1, wherein the LIM mineralization protein is HLMP-1s (SEQ ID NO: 34).12. The method of claim 9, wherein the isolated nucleic acid molecule is in a vector selected from the group consisting of a plasmid and a virus.13. The method of claim 12, wherein the vector is a plasmid, which plasmid is directly injected into muscle tissue.14. A method of stimulating production of an osteogenic soluble factor by an osteogenic cell, comprising:(a) transfecting the osteogenic cell with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein; and (b) overexpressing the isolated nucleic acid molecule, wherein said nucleic acid molecule is SEQ ID NO: 22 or SEQ ID NO: 33.

이 특허에 인용된 특허 (242)

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상세보기
March Keith L. (Carmel IN) Hathaway David R. (Indianapolis IN) Wilensky Robert L. (Indianapolis IN), Method for delivery of smooth muscle cell inhibitors.
상세보기
Hermes Matthew E. (Easton CT) Muth Ross R. (Brookfield CT), Method for improving the storage stability of a polymeric article susceptible to hydrolytic degradation and resulting ar.
상세보기
Lagarde Alain E. (Kingston CAX) Abdulwajid Abdulwahid (Toronto CAX) Bueschkens Donna (Mississauga CAX) Byrne Deanna (Scarborough CAX), Method for isolating a soluble osteogenic factor from bone.
상세보기
Mathiowitz Edith (Brookline MA) Bernstein Howard (Cambridge MA) Morrel Eric (Needham MA) Schwaller Kirsten (Duxbury MA), Method for producing protein microspheres.
상세보기
Eppley Barry L. (7702C Chaterly Ct. Indianapolis IN 46214) Krukowski Marilyn D. (24 Washington Terrace St. Louis MO 63112) Osdoby Philip A. (134 Holly Garden Dr. Ballwin MO 63021), Method for promoting hard tissue growth and repair in mammals.
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Oppermann Hermann (Medway MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (West Roxbury MA) Ozkaynak Engin (Milford MA), Method for recombinant production of osteogenic protein.
상세보기
Yang Na N. (Indianapolis IN), Method for screening anti-osteoporosis agents.
상세보기
Tice Thomas R. (Birmingham AL) Dillon Deborah L. (Helena AL) Mason David W. (Birmingham AL) McRae-McFarlane Amanda (Vastra Frolunda SEX) Dahlstrom Annica B. (Askin SEX), Method for stimulating nerve fiber growth.
상세보기
Brizzolara Nancy S. (Congers NY) Lanzilotti Michael G. (Pearl River NY) Lawter James R. (Goshen NY), Method for the treatment of periodontal disease by sustained delivery of a therapeutic agent to the periodontal pocket,.
상세보기
Tam Cherk S. (1072 Rectory Lane Oakville ; Ontario L6M 2B7 CAX), Method of increasing bone growth.
상세보기
Neer Robert M. (Cambridge MA) Potts ; Jr. John T. (Newton MA) Slovik David M. (Newton MA), Method of increasing bone mass.
상세보기
Jefferies Steven R. (5802 Leith Walk Baltimore MD 21239), Method of inducing osseous formation by implanting bone graft material.
상세보기
Szoka ; Jr. Francis C. (76 Summit St. Waltham MA 02154) Papahadjopoulos Demetrios P. (3170 Condit St. Lafayette CA 94549), Method of inserting DNA into living cells.
상세보기
Oppermann Hermann ; Ozkaynak Engin ; Kuberasampath Thangavel ; Rueger David C. ; Pang Roy H. L., Method of selectively extracting osteogenic protein.
상세보기
Bentz Hanne (Palo Alto CA) Ellingsworth Larry (San Jose CA) Armstrong Rosa (Palo Alto CA), Method of treating inflammation.
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Oppermann Hermann ; Dorai Haimanti ; Kaplan Paul, Methods and compositions for high protein production form recombinant DNA.
상세보기
Oppermann Hermann (Medway MA) Dorai Haimanti (Lexington MA) Kaplan Paul (Auburndale MA), Methods and compositions for high protein production from recombinant DNA.
상세보기
Oppermann Hermann (Medway MA) Dorai Haimanti (Lexington MA) Kaplan Paul (Auburndale MA), Methods and compositions for high protein production from recombinant DNA.
상세보기
Hunziker Ernst B. (Riedholz CHX), Methods and compositions for the treatment and repair of defects or lesions in cartilage.
상세보기
Desai Neil P. (Los Angeles CA) Soon-Shiong Patrick (Los Angeles CA) Sandford Paul A. (Los Angeles CA) Grinstaff Mark W. (Pasadena CA) Suslick Kenneth S. (Champaign IL), Methods for in vivo delivery of substantially water insoluble pharmacologically active agents and compositions useful th.
상세보기
Nimberg Richard B. (Sharon MA) Colclasure Craig (Watertown MA) Lloyd Weldon S. (Sharon MA), Methods for inducing general and localized bone apposition in-vivo.
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Wozney John M. (Hudson MA) Wang Elizabeth A. (Carlisle MA) Rosen Vicki A. (Brookline MA), Methods for producing BMP-7 proteins.
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Allard Roland (St Genis Laval FRX) Huc Alain (Ste Foy Les Lyon FRX), Microcapsules having a combined atelocollagen/polyholoside wall coagulated by a divalent cation and method for manufactu.
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Soon-Shiong Patrick ; DeSai Neil P. ; Sandford Paul A. ; Heintz Roswitha A. ; Sojomihardjo Soebianto, Microcapsules prepared from crosslinkable polysaccharides, polycations and/or lipids and use therefor.
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Kuberasampath Thangavel (Medway MA) Rueger David C. (Hopkinton MA) Oppermann Hermann (Medway MA) Cohen Charles M. (Medway MA) Pang Roy H. L. (Etna NH), Morphogen induced periodontal tissue regeneration.
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Smart John E. (Weston MA) Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (Hopkinton MA) Pang Roy H.L. (Etna NH) Cohen Charles M. (Medway, Morphogenic protein screening method.
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Schwartz Robert J. (Houston TX) Chow King L. (Houston TX), Mutated skeletal actin promoter.
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Schwartz Robert J. (Houston TX) DeMayo Franco J. (Houston TX) O\Malley Bert W. (Houston TX), Myogenic vector systems.
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Cardinaux Franois (Seewen CHX) Oechslein Christine (Kaiseraugst CHX) Rummelt Andreas (Oberwil CHX), Nasal pharmaceutical composition.
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Vacanti Joseph P. (Winchester MA) Vacanti Charles A. (Lexington MA) Langer Robert S. (Newton MA), Neomorphogenesis of cartilage in vivo from cell culture.
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Vert Michel (Deville-les-Rouen FRX) Chabot Francois (Rouen FRX) Leray Jean (Sevres FRX) Christel Pascal (Paris FRX), New prosthesis parts, their preparation and their application.
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Derynck Rik M. A. ; Goeddel David V., Nucleic acid encoding TGF-.beta. and its uses.
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O\Leary Robert K. (Spring Lake NJ) Prewett Annamarie B. (Little Silver NJ), Osteogenic composition and implant containing same.
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Kuberasampath Thangavel (Medway MA) Rueger David C. (West Roxbury MA), Osteogenic devices.
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Oppermann Hermann (Medway MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (West Roxbury MA) Ozkaynak Engin (Milford MA), Osteogenic devices.
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Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (Hopkinton MA) Pang Roy H. L. (Medway MA), Osteogenic devices.
상세보기
Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA) Rueger David C. (Hopkinton MA) Kuberasampath Thangavel (Medway MA), Osteogenic devices.
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Parsons Thomas F. (Arcadia CA) Sen Arup (Van Nuys CA) Grinna Lynn (Santa Monica CA) Hersh Carol (Great Neck NY) Theofan Georgia (Los Angeles CA), Osteogenic factors.
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Sen Arup (Los Angeles CA), Osteogenic factors.
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Rodan Gideon A. (Bryn Mawr PA) Jacobs John W. (Irvine CA) Sardana Mohinder K. (Lansdale PA) Gazit Dan (Jerusalem ILX) Chorev Michael (Jerusalem ILX) Muhlrad Andras (Jerusalem ITX) Shteyer Arye (Jerus, Osteogenic growth polypeptides identified from regenerating bone marrow.
상세보기
Oppermann Hermann (Medway MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (West Roxbury MA) Ozkaynak Engin (Milford MA), Osteogenic proteins.
상세보기
Oppermann Hermann (Medway MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (West Roxbury MA) Ozkaynak Engin (Milford MA), Osteogenic proteins.
상세보기
Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA) Kuberasampath Thangavel (Medway MA) Rueger David C. (Hopkinton MA) Pang Roy H. L. (Medway MA), Osteogenic proteins.
상세보기
Kuberasampath Thangavel (Medway MA) Rueger David C. (West Roxbury MA), Osteogenic proteins and polypeptides.
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Wang Elizabeth A. (Carlisle MA) Wozney John M. (Hudson MA) Rosen Vicki (Boston MA), Osteoinductive factors.
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Ikada Yoshito (Uji JPX) Suong Hyu H. (Uji JPX) Shimizu Yoshihiko (Uji JPX) Watanabe Satoshi (Kyoto JPX) Nakamura Tatsuo (Kyoto JPX) Suzuki Masakazu (Ayabe JPX) Shimamoto Takeshi (Nagaokakyo JPX), Osteosynthetic pin.
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Pang Peter K. T. (University of Alberta ; Dept. of Physiology ; 7-55 Medical Science Bldg. Edmonton CAX T6G 2H7) Shan Jie (Edmonton CAX), Parathyroid hormone analogues substituted at AA 25, 26, 27, and use in osteoporosis treatment.
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Leonard Robert J. (Lynnfield MA) Harman S. Mitchell (Ellicott City MD), Partially fused peptide pellet.
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Seyedin Saeid (Mountain View CA) Thomas Thomas (Davis CA), Partially purified bone-inducing factor.
상세보기
Seyedin Saeid (Mt. View CA) Thomas Thomas (Palo Alto CA), Partially purified osteogenic factor and process for preparing same from demineralized bone.
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McCoy John (Reading MA) DiBlasio-Smith Elizabeth (Tyngsboro MA) Grant Kathleen (Salem MA) LaVallie Edward R. (Tewksbury MA), Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules.
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Grinnell Frederick (Dallas TX), Peptide inhibitors of wound contraction.
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Jernberg Gary R. (99 Navaho Ave. Suite 102 Mankato MN 56001), Periodontal barrier and method for aiding periodontal tissue regeneration.
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Ikada Yoshito (Uji JPX) Gen Shokyu (Kyoto JPX), Periodontium-regenerative materials.
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Ron Eyal (Lexington MA) Turek Thomas J. (Boston MA) Isaacs Benjamin S. (Tewksbury MA) Patel Himakshi (Tewksbury MA) Kenley Richard A. (Andover MA), Pharmaceutical formulations of osteogenic proteins.
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Prisell Per (Wollmar Yxkullsgatan 15A S-116 50 Stockholm SEX) Norstedt Gunnar (Foerfattavaegen 46 S-116 42 Bromma SEX), Pharmaceutical preparation.
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Hollinger Jeffrey O. (Glenwood MD), Polylactic-polyglycolic acids combined with an acidic phospholipid-lysozyme complex for healing osseous tissue.
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Mathiowitz Edith (Brookline MA) Langer Robert S. (Somerville MA) Warshawsky Abraham (Rehovot MA ILX) Edelman Elazar (Brookline MA), Polymer composite for controlled release or membrane formation.
상세보기
Jarrett Peter K. (Southbury CT) Casey Donald J. (Mars PA) Rosati Louis (Norwalk CT) Dwyer James W. (Brookfield CT), Polymeric surgical staple.
상세보기
Findlay Christopher J. (Cambridge CAX), Porous immobilization support prepared from animal bone.
상세보기
DeLuca Patrick P. (Lexington KY) Kanke Motoko (Fukuyama JPX) Sato Toyomi (Tokyo CA JPX) Schroeder Hans G. (Encinitas CA), Porous microspheres for drug delivery and methods for making same.
상세보기
Ramstack J. Michael (Lebanon OH) Herbert Paul F. (Wayland MA) Strobel Jan (Westchester OH) Atkins Thomas J. (Cincinnati OH), Preparation of biodegradable microparticles containing a biologically active agent.
상세보기
Morse Brenda S. (Chamblee GA) Dioh Clement D. (Marietta GA), Preparation of bone for transplantation.
상세보기
Chu George (Sunnyvale CA), Preparation of collagen hydroxyapatite matrix for bone repair.
상세보기
Rickey Michael E. ; Ramstack J. Michael ; Lewis Danny H. ; Mesens Jean,BEX, Preparation of extended shelf-life biodegradable, biocompatible microparticles containing a biologically active agent.
상세보기
Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences.
상세보기
Kaplan Donald S. (Weston CT) Hermes Matthew E. (Norwalk CT) Muth Ross R. (Brookfield CT) Kennedy John J. (Stratford CT), Process for making a surgical device using two-phase compositions.
상세보기
Fong, Jones W., Process for preparation of microspheres.
상세보기
Prewett Annamarie B. (Little Silver NJ) Stikeleather Roger C. (Doylestown PA), Process for producing flowable osteogenic composition containing demineralized bone particles.
상세보기
Kaplan Donald S. (Weston CT) Hermes Matthew E. (Norwalk CT) Muth Ross R. (Brookfield CT) Kennedy John J. (Stratford CT), Process of making an absorbable surgical device.
상세보기
Fong Jones W. (Parsippany NJ), Processes for preparation of microspheres.
상세보기
Chu George H. (Sunnyvale CA) Ogawa Yasushi (Pacifica CA) McPherson John M. (Framingham MA) Ksander George (Redwood City CA) Pratt Bruce (Union City CA) Hendricks Diana (Brea CA) McMullin Hugh (San Br, Processes for producing collagen matrixes and methods of using same.
상세보기
Moos ; Jr. Malcolm (Bethesda MD) Krinks Marie (Rockville MD) Wang Shouwen (Rockville MD), Production and use of anti-dorsalizing morphogenetic protein.
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Israel David I. (Concord MA), Production of recombinant bone-inducing proteins.
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Rueger David C. (Hopkinton MA) Kuberasampath Thangavel (Medway MA) Oppermann Hermann (Medway MA) Ozkaynak Engin (Milford MA), Prosthetic devices having enhanced osteogenic properties.
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Bernstein Howard (Cambridge MA) Morrel Eric (Needham MA) Mathiowitz Edith (Brookline MA) Schwaller Kirsten (Duxbury MA) Beck Thomas R. (Concord MA), Protein microspheres and methods of using them.
상세보기
Kronenberg Henry M. (Belmont MA) Nussbaum Samuel R. (Weston MA) Doi Tomoko (Osaka JPX), Recombinant DNA method for production of parathyroid hormone.
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Kandel Rita A. (Toronto CAX), Reconstituted cartridge tissue.
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Fues Johann-Friedrich (Grevenbroich DEX) Ritter Wolfgang (Haan DEX), Resorbable bone wax.
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Fues Johann-Friedrich (Grevenbroich DEX) Ritter Wolfgang (Haan DEX), Resorbable bone wax.
상세보기
Miller A. Dusty (Seattle WA) Palmer Theo D. (Bellevue WA), Retroviral gene transfer into diploid fibroblasts for gene therapy.
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Prewett Annamarie B. (Little Silver NJ) Stikeleather Roger C. (Doylestown PA) Bogdansky Simon (Marlboro NJ) O\Leary Robert K. (Spring Lake NJ), Shaped, swollen demineralized bone and its use in bone repair.
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Li Shu-Tung (1 Kiowa Ter. Oakland NJ 07436), Soft tissue closure systems.
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Ledley Fred D. ; O'Malley ; Jr. Bert W., Somatic gene therapy to cells associated with fluid spaces.
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Kaplan Donald S. (Weston CT) Kennedy John (Stratford CT) Muth Ross R. (Brookfield CT) Gruskin Elliot A. (Killingworth CT), Support material for cell impregnation.
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Jamiolkowski Dennis D. (Long Valley NJ) Gaterud Mark T. (Annandale NJ) Newman ; Jr. Hugh D. (Chester NJ) Shalaby Shalaby W. (Lebanon NJ), Surgical fastener made from glycolide-rich polymer blends.
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Smith Carl R. (Bloomingdale NJ) Gaterud Mark T. (Annandale NJ) Jamiolkowski Dennis D. (Long Valley NJ) Newman ; Jr. Hugh D. (Chester NJ) Shalaby Shalaby W. (Lebanon NJ), Surgical fastener made from glycolide-rich polymer blends.
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Orsolini Piero (Martigny) Mauvernay Rolland-Yves (Lausanne) Deghenghi Romano (Lausanne CHX), Sustained and controlled release of water insoluble polypeptides.
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Fujioka Keiji (Ibaraki JPX) Sato Shigeji (Ibaraki JPX) Takada Yoshihiro (Ibaraki JPX) Sasaki Yoshio (Ibaraki JPX) Tamura Nobuhiko (Ibaraki JPX), Sustained pulsewise release pharmaceutical preparation.
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Bogdansky Simon (Marlboro NJ) O\Leary Robert K. (Spring Lake NJ), Swollen demineralized bone particles, flowable osteogenic composition containing same and use of the composition in the.
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Stadler Joan K. (3313 Oakland St. Ames IA 50010) Antonelli Nunzio M. (via Pezze del Sole ; 24 Bari ITX 70125), Synergistic method for host cell transformation.
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Kuberasampath Thangavel (Medway MA) Tarrant Lawrence Berlowitz (Harvard MA), Synthetic bone matrix.
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Rosensaft Michael N. (Monsey NY) Webb Richard L. (Darien CT), Synthetic polyester surgical articles.
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Rosensaft Michael N. (Monsey NY) Webb Richard L. (Darien CT), Synthetic polyester surgical articles.
상세보기
Ammann Arthur J. (San Rafael CA) Rudman Christopher G. (San Francisco CA), TGF-b 상세보기

IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Bone mineralization proteins, DNA, vectors expression systems 원문보기

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Bone mineralization proteins, DNA, vectors expression systems 원문보기

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