Preservation and storage medium for biological materials
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-001/00
A01N-001/02
A61K-031/7012
출원번호
US-0657017
(2003-09-05)
발명자
/ 주소
DePablo, Juan
Miller, Danforth
Conrad, Paul
Corti, Horacio
출원인 / 주소
Wisconsin Alumni Research Foundation
대리인 / 주소
DeWitt Ross &
인용정보
피인용 횟수 :
65인용 특허 :
19
초록▼
A protectant mixture for use in preserving biological materials comprising (1) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the mixture is from about 5% to about 60% by weight of the mixture where the mixture is an aqueous solution and is from about 10% to abo
A protectant mixture for use in preserving biological materials comprising (1) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the mixture is from about 5% to about 60% by weight of the mixture where the mixture is an aqueous solution and is from about 10% to about 95% where the mixture is in solid form, and (2) phosphate ions, where the total amount of phosphate ions in the mixture is such that the molar ratio of phosphate ions to hydroxy groups in the polyhydroxy compound is from about 0.025 to about 0.625; a preservation medium comprising (1) a biological material, (2) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the medium is from about 5% to about 60% by weight of the medium, and (3) phosphate ions, where the total amount of phosphate ions in the mixture is such that the molar ratio of phosphate ions to hydroxy groups in the polyhydroxy compound is from about 0.025 to about 0.625; methods of preserving the preservation medium; and the resulting preserved biological material composition.
대표청구항▼
1. A method of preparing a preserved biological material composition comprising:(a) forming an aqueous preservation medium comprising (i) a biological material; (ii) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the medium is from 10% to about 60% by weight of
1. A method of preparing a preserved biological material composition comprising:(a) forming an aqueous preservation medium comprising (i) a biological material; (ii) at least one polyhydroxy compound, where the total amount of polyhydroxy compound in the medium is from 10% to about 60% by weight of the medium; and (iii) phosphate ions, where the total amount of phosphate ions in the medium is such that the molar ratio of phosphate ions to moles of hydroxyl groups in the polyhydroxy compound is from about 0.025 to about 0.625; and (b) preserving the aqueous preservation medium using at least one preservation process. 2. The method of claim 1, where the preservation processes are one or more processes selected from the group consisting of freezing, freeze-drying, ambient-air drying, vacuum-drying, and spray-drying.3. The method of claim 1, where the pH of the medium is about 5 to about 10.4. The method of claim 1, where the polyhydroxy compound is selected from a group consisting of monosaccharides, disaccharides, and polysaccharides.5. The method of claim 1, where the polyhydroxy compound is trehalose.6. The method of claim 1, where the total amount of polyhydroxy compound in the medium is from 10% to about 30% by weight of the medium.7. The method of claim 4, where the total amount of polyhydroxy compound in the medium is from 10% to about 30% by weight of the medium.8. The method of claim 1, where the molar ratio of phosphate ions to moles of hydroxyl groups in the polyhydroxy compound is from about 0.0375 to about 0.625.9. The method of claim 1, where the biological material is selected form the group consisting of cells, proteins, and enzymes.10. A method of preparing a preserved biological material composition comprising:(a) forming an aqueous preservation medium comprising (i) a biological material; (ii) trehalose, where the total amount trehalose in the medium is from 10% to about 60% by weight of the medium; and (iii) phosphate ions, where the total amount of phosphate ions in the medium is such that the molar ratio of phosphate ions to trehalose is from about 0.2 to about 5; and (b) preserving the aqueous preservation medium using at least one preservation process. 11. The method of claim 10, where the preservation processes are one or more processes selected from the group consisting of freezing, freeze-drying, ambient-air drying, vacuum-drying, and spray-drying.12. The method of claim 10, where the trehalose is present in an amount from 10% to about 30% by weight of the medium.13. The method of claims 12, where the pH is from about 5 to about 10.14. The method of claim 13, where the molar ratio of phosphate ions to trehalose is from about 0.3 to about 5.15. The method of claim 10, where the biological material is selected from the group consisting of cells, proteins, and enzymes.
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