IPC분류정보
| 국가/구분 |
United States(US) Patent
등록
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| 국제특허분류(IPC7판) |
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| 출원번호 |
US-0356349
(2003-01-31)
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발명자
/ 주소 |
- Venkataraman, Ganesh
- Shriver, Zachary
- Raman, Rahul
- Sasisekharan, Ram
- Keiser, Nishla
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| 출원인 / 주소 |
- Massachusetts Institute of Technology
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| 대리인 / 주소 |
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| 인용정보 |
피인용 횟수 :
0 인용 특허 :
52 |
초록
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The invention relates to methods of identifying and characterizing properties of polymers to provide information about the polymer such as the charge of the polymer, the number and types or characteristics of units of the polymer and the sequence of the polymers. The invention also relates to method
The invention relates to methods of identifying and characterizing properties of polymers to provide information about the polymer such as the charge of the polymer, the number and types or characteristics of units of the polymer and the sequence of the polymers. The invention also relates to methods of sequencing polymers such as nucleic acids, polypeptides and polysaccharides and methods for identifying a polysaccharide-protein interaction.
대표청구항
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1. In a system including a database of values of properties of polymers of chemical units, a method for determining the composition of a sample polymer of chemical units having a known molecular length, comprising steps of:(A) selecting, from the database, candidate polymers of chemical units having
1. In a system including a database of values of properties of polymers of chemical units, a method for determining the composition of a sample polymer of chemical units having a known molecular length, comprising steps of:(A) selecting, from the database, candidate polymers of chemical units having the same length as the sample polymer of chemical units and for which the value of a predetermined property is similar to the value of the predetermined property of the sample polymer of chemical units; (B) performing an experiment on the sample polymer of chemical units; (C) measuring properties of the sample polymer of chemical units resulting from the experiment; and (D) eliminating, from the candidate polymers of chemical units, polymers of chemical units having properties that do not correspond to the experimental results. 2. The method of claim 1, further comprising a step of:(E) repeatedly performing the step (D) until the number of candidate polymers of chemical units falls below a predetermined threshold. 3. The method of claim 1, wherein the predetermined property is molecular weight.4. The method of claim 1, wherein the sample polymer is a polysaccharide.5. The method of claim 4, wherein the polysaccharide is a heparin-like-glycosaminoglycan.6. The method of claim 1, wherein the step of performing an experiment involves applying an experimental constraint to the sample polymer.7. The method of claim 6, wherein the experimental constraint is selected from the group consisting of enzymatic digestion, chemical digestion, chemical modification, interaction with a binding compound, chemical peeling, and enzymatic modification.8. The method of claim 7, wherein the enzymatic digestion is selected from the group consisting of digestion with an exoenzyme, an endoenzyme, and a restriction endonuclease.9. The method of claim 7, wherein the enzymatic modification is sulfation.10. A method for identifying a population of polymers of chemical units having the same property as a sample polymer of chemical units, comprising:determining a property of a sample polymer of chemical units; comparing the property of the sample polymer to a reference database of polymers of known sequence and known properties to identify a population of polymers of chemical units having the same property as a sample polymer of chemical units, wherein the reference database of polymers includes identifiers corresponding to the chemical units of the polymers, each of the identifiers including a field storing a value corresponding to the property, and wherein the sample polymer is a polysaccharide. 11. The method of claim 10, wherein the step of determining a property of the sample polymer involves the use of mass spectrometry to determine the molecular weight of the polymer.12. The method of claim 11, wherein the mass spectrometry is MALDI which detects molecular weight with an accuracy of approximately one Dalton.13. The method of claim 12, wherein the MALDI analysis is performed on a MALDI surface having a protein coated thereon.14. The method of claim 12, wherein the sample polymer is isolated from a cell surface.15. The method of claim 10, wherein the sample polymer is reduced to at least two fragments and the property of the sample polymer is the size of the fragments, and wherein the step of determining a property involves strong ion exchange chromatography.16. The method of claim 10, wherein the polysaccharide is a heparin-like-glycosaminoglycan.17. The method of claim 10, wherein the step of determining a property involves applying an experimental constraint to the sample polmer.18. The method of claim 17, wherein the experimental constraint is selected from the group consisting of enzymatic digestion, chemical digestion, chemical modification, interaction with a binding compound, chemical peeling, and enzymatic modification.19. The method of claim 18, wherein the enzymatic digestion is selected from the group consisting of digestion with an exoenzyme, an endoenzyme, and a restriction endonuclease.20. The method of claim 18, wherein the enzymatic modification is sulfation.21. A method for identifying a population of polymers of chemical units having the same property as a sample polymer of chemical units, comprising:determining a property of a sample polymer of chemical units; comparing the property of the sample polymer to a reference database of polymers of known sequence and known properties to identify a population of polymers of chemical units having the same property as a sample polymer of chemical units, wherein the reference database of polymers includes identifiers corresponding to the chemical units of the polymers, each of the identifiers including a field storing a value corresponding to the property, and wherein the step of determining a property involves the use of MALDI which detects molecular weight with an accuracy of approximately one Dalton. 22. A method for identifying a population of polymers of chemical units having the same property as a sample polymer of chemical units, comprising:determining a property of a sample polymer of chemical units; comparing the property of the sample polymer to a reference database of polymers of known sequence and known properties to identify a population of polymers of chemical units having the same property as a sample polymer of chemical units, wherein the reference database of polymers includes identifiers corresponding to the chemical units of the polymers, each of the identifiers including a field storing a value corresponding to the property, and wherein the sample polymer is reduced to at least two fragments and the property of the sample polymer is the size of the fragments and the step of determining a property involves strong ion exchange chromatography. 23. A method for identifying a population of polymers of chemical units having the same property as a sample polymer of chemical units, comprising:determining a property of a sample polymer of chemical units; comparing the property of the sample polymer to a reference database of polymers of known sequence and known properties to identify a population of polymers of chemical units having the same property as a sample polymer of chemical units, wherein the reference database of polymers includes identifiers corresponding to the chemical units of the polymers, each of the identifiers including a field storing a value corresponding to the property, and wherein the step of determining a property involves applying to the sample polymer an experimental constraint selected from the group consisting of enzymatic digestion, chemical digestion, chemical modification, interaction with a binding compound, chemical peeling, and enzymatic modification.
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