Plant retroelements and methods related thereto
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/04
C12N-015/867
C12N-005/14
A01H-001/00
출원번호
US-0799870
(2004-03-12)
발명자
/ 주소
Wright, David A.
Voytas, Daniel F.
출원인 / 주소
Iowa State University Research Foundation, Inc.
대리인 / 주소
Fish &
인용정보
피인용 횟수 :
0인용 특허 :
13
초록▼
The present invention provides plant retroelements useful as molecular tools. In one embodiment, the present invention provides nucleic acids encoding gag, pol and/or env genes of plant retroelements. The elements can be used, among other uses, as building blocks of other constructs, tools to find o
The present invention provides plant retroelements useful as molecular tools. In one embodiment, the present invention provides nucleic acids encoding gag, pol and/or env genes of plant retroelements. The elements can be used, among other uses, as building blocks of other constructs, tools to find other nucleic acid sequences and tools to transfer nucleic acid into cells.
대표청구항▼
1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:(a) a sequence having at least 85% identity to SEQ ID NO 62, (b) a sequence encoding a polypeptide comprising an amino acid sequence having at least 85% identity to SEQ ID NO 63, and (c) a seq
1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:(a) a sequence having at least 85% identity to SEQ ID NO 62, (b) a sequence encoding a polypeptide comprising an amino acid sequence having at least 85% identity to SEQ ID NO 63, and (c) a sequence fully complementary to (a) or (b). 2. The nucleic acid molecule of claim 1, said nucleic acid further comprising a gag coding sequence and an env coding sequence, wherein adenine-thymidine-guanidine is the gag coding sequence start codon.3. The nucleic acid molecule of claim 1, wherein a pol coding sequence comprises said nucleic acid.4. A transformed seed containing a recombinant construct comprising the nucleic acid of claim 1.5. The isolated nucleic acid molecule of claim 1, wherein said nucleotide sequence is selected from the group consisting of:(a) a sequence having at least 95% identity to SEQ ID NO 62, (b) a sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO 63, and (c) a sequence fully complementary to (a) or (b). 6. The nucleic acid molecule of claim 5, said nucleic acid further comprising a gag coding sequence and an env coding sequence, wherein adenine-thymidine-guanidine is the gag coding sequence start codon.7. The nucleic acid molecule of claim 5, wherein a pol coding sequence comprises said nucleic acid.8. A transformed seed containing a recombinant construct comprising the nucleic acid of claim 5.9. A transformed plant comprising a recombinant nucleic acid construct, said construct comprising a nucleotide sequence selected from the group consisting of:(a) a sequence having at least 85% identity to SEQ ID NO 62, (b) a sequence encoding a polypeptide comprising an amino acid sequence having at least 85% identity to SEQ ID NO 63, and (c) a sequence fully complementary to (a) or (b). 10. The transformed plant of claim 9, wherein said construct comprises a nucleotide sequence having at least 85% identity to SEQ ID NO 62.11. The transformed plant of claim 9, wherein said construct comprises a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 85% identity to SEQ ID NO 63.12. The transformed plant of claim 9, wherein said plant is selected from the group consisting of: soybean, maize, sugar cane, beet, tobacco, wheat, barley, poppy, rape, sunflower, alfalfa, sorghum, rose, carnation, gerbera, carrot, tomato, lettuce, chicory, pepper, melon, cabbage, oat, rye, cotton, flax, potato, pine, walnut, citrus, hemp, oak, rice, petunia, orchids, Arabidopsis, broccoli, cauliflower, brussel sprouts, onion, garlic, leek, squash, pumpkin, celery, pea, bean, strawberries, grapes, apples, pears, peaches, banana, palm, cocoa, cucumber, pineapple, apricot, plum, sugar beet, lawn grasses, maple, triticale, safflower, peanut, and olive.13. A transformed plant comprising a recombinant nucleic acid construct, said construct comprising a nucleotide sequence selected from the group consisting of:(a) a sequence having at least 95% identity to SEQ ID NO 62, (b) a sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO 63, and (c) a sequence fully complementary to (a) or (b). 14. The transformed plant of claim 13, wherein said construct comprises a nucleotide sequence having at least 95% identity to SEQ ID NO 62.15. The transformed plant of claim 13, wherein said construct comprises a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO 63.16. The transformed plant of claim 13, wherein said plant is selected from the group consisting of: soybean, maize, sugar cane, beet, tobacco, wheat, barley, poppy, rape, sunflower, alfalfa, sorghum, rose, carnation, gerbera, carrot, tomato, lettuce, chicory, pepper, melon, cabbage, oat, rye, cotton, flax, potato, pine, walnut, citrus, hemp, oak, rice, petunia, orchids, Arabidopsis, broccoli, cauliflower, brussel sprouts, onion, garlic, leek, squash, pumpkin, celery, pea, bean, strawberries, grapes, apples, pears, peaches, banana, palm, cocoa, cucumber, pineapple, apricot, plum, sugar beet, lawn grasses, maple, triticale, safflower, peanut, and olive.
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